Lin, Yu-Shih; Biddle, Jennifer F; Lipp, Julius S; Orcutt, Beth N; Holler, Thomas; Teske, Andreas P; Hinrichs, Kai-Uwe (2010): Oxygen contents, sulfate concentrations, and changes of IPLs under different storage conditions of samples from ODP Leg 204 and IODP Expedition 311 [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.745155, Supplement to: Lin, Yu-Shih; Biddle, Jennifer F; Lipp, Julius S; Orcutt, B; Holler, Thomas; Teske, Andreas P; Hinrichs, Kai-Uwe (2010): Effect of storage conditions on archaeal and bacterial communities in subsurface marine sediments. Geomicrobiology Journal, 27, 261-272, https://doi.org/10.1080/01490450903410423
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Abstract:
We have studied the effects of slow infiltration of oxygen on microbial communities in refrigerated legacy samples from ocean drilling expeditions. Storage was in heat-sealed, laminated foil bags with a N2 headspace for geomicrobiological studies. Analysis of microbial lipids suggests that Bacteria were barely detectable in situ but increased remarkably during storage. Detailed molecular examination of a methane-rich sediment horizon showed that refrigeration triggered selective growth of ANME-2 archaea and a drastic change in the bacterial community. Subsequent enrichment targeting methanogens yielded exclusively methylotrophs, which were probably selected for by high sulfate levels caused by oxidation of reduced sulfur species. We provide recommendations for sample storage in future ocean drilling expeditions.
Related to:
Lin, Yu-Shih (2009): In vitro study of microbial carbon cycling in subseafloor sediments [dissertation]. Staats- und Universitätsbibliothek Bremen, Germany, urn:nbn:de:gbv:46-diss000117097
Project(s):
Coverage:
Median Latitude: 47.466999 * Median Longitude: -126.358755 * South-bound Latitude: 44.568530 * West-bound Longitude: -127.000845 * North-bound Latitude: 48.789583 * East-bound Longitude: -125.073930
Date/Time Start: 2002-07-21T00:00:00 * Date/Time End: 2002-08-29T00:00:00
Event(s):
204-1249 * Latitude: 44.570317 * Longitude: -125.147333 * Date/Time Start: 2002-07-21T00:00:00 * Date/Time End: 2002-08-29T00:00:00 * Elevation: -777.2 m * Penetration: 618.7 m * Recovery: 224.7 m * Location: North Pacific Ocean * Campaign: Leg204 * Basis: Joides Resolution * Method/Device: Composite Core (COMPCORE) * Comment: 72 cores; 484.8 m cored; 104 m drilled; 46.3 % recovery
204-1249F * Latitude: 44.570530 * Longitude: -125.147300 * Date/Time Start: 2002-08-05T08:30:00 * Date/Time End: 2002-08-06T07:15:00 * Elevation: -777.2 m * Penetration: 90 m * Recovery: 57.37 m * Location: North Pacific Ocean * Campaign: Leg204 * Basis: Joides Resolution * Method/Device: Drilling/drill rig (DRILL) * Comment: 16 cores; 82.5 m cored; 7.5 m drilled; 69.5 % recovery
204-1250 * Latitude: 44.568600 * Longitude: -125.150183 * Date/Time Start: 2002-07-21T00:00:00 * Date/Time End: 2002-08-27T00:00:00 * Elevation: -795.8 m * Penetration: 876 m * Recovery: 332 m * Location: North Pacific Ocean * Campaign: Leg204 * Basis: Joides Resolution * Method/Device: Composite Core (COMPCORE) * Comment: 53 cores; 378 m cored; 498 m drilled; 87.8 % recovery
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
5 datasets
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Datasets listed in this publication series
- Lin, Y-S; Biddle, JF; Lipp, JS et al. (2010): (Figure 2) Changes of IPLs in subsurface sediments from ODP Leg 204, Hydrate Ridge, under different storage conditions. https://doi.org/10.1594/PANGAEA.745152
- Lin, Y-S; Biddle, JF; Lipp, JS et al. (2010): (Figure 3) Changes of IPLs in subsurface sediments from IODP Expedition 311, Cascadia Margin, under different storage conditions. https://doi.org/10.1594/PANGAEA.745154
- Lin, Y-S; Biddle, JF; Lipp, JS et al. (2010): (Figure 4) Comparison of microbial communities in subsurface sediment from Sample 1328-152.7 after different storage conditions or incubation with methanol at room temperature. https://doi.org/10.1594/PANGAEA.756693
- Lin, Y-S; Biddle, JF; Lipp, JS et al. (2010): (Table 1) Oxygen contents and sulfate concentrations from shipboard and shore-based measurements of some refrigerated samples from IODP Expedition 311 and ODP Leg 204 (after 708 days of storage). https://doi.org/10.1594/PANGAEA.756632
- Lin, Y-S; Biddle, JF; Lipp, JS et al. (2010): (Table 2) Quantitative PCR detection of mcrA genes and intact polar archaeol and hydroxyarchaeol in sample 1328-152.7 under different storage conditions and after incubation with methanol at room temperature. https://doi.org/10.1594/PANGAEA.756669