Citation:

01490450903410423

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Abstract:

We have studied the effects of slow infiltration of oxygen on microbial communities in refrigerated legacy samples from ocean drilling expeditions. Storage was in heat-sealed, laminated foil bags with a N2 headspace for geomicrobiological studies. Analysis of microbial lipids suggests that Bacteria were barely detectable in situ but increased remarkably during storage. Detailed molecular examination of a methane-rich sediment horizon showed that refrigeration triggered selective growth of ANME-2 archaea and a drastic change in the bacterial community. Subsequent enrichment targeting methanogens yielded exclusively methylotrophs, which were probably selected for by high sulfate levels caused by oxidation of reduced sulfur species. We provide recommendations for sample storage in future ocean drilling expeditions.

Related to:

Lin, Yu-Shih (2009): In vitro study of microbial carbon cycling in subseafloor sediments. PhD Thesis, Elektronische Dissertationen an der Staats- und Universitätsbibliothek Bremen, Germany, urn:nbn:de:gbv:46-diss000117097

Project(s):

Center for Marine Environmental Sciences (MARUM)

Integrated Ocean Drilling Program / International Ocean Discovery Program (IODP)

Ocean Drilling Program (ODP)

Coverage:

Median Latitude: 47.466999 * Median Longitude: -126.358755 * South-bound Latitude: 44.568530 * West-bound Longitude: -127.000845 * North-bound Latitude: 48.789583 * East-bound Longitude: -125.073930

Date/Time Start: 2002-07-21T00:00:00 * Date/Time End: 2002-08-29T00:00:00

Event(s):

204-1249 * Latitude: 44.570317 * Longitude: -125.147333 * Date/Time Start: 2002-07-21T00:00:00 * Date/Time End: 2002-08-29T00:00:00 * Elevation: -777.2 m * Penetration: 618.7 m * Recovery: 224.7 m * Location: North Pacific Ocean * Campaign: * Basis: Joides Resolution * Method/Device: Composite Core (COMPCORE) * Comment: 72 cores; 484.8 m cored; 104 m drilled; 46.3 % recovery

204-1249F * Latitude: 44.570530 * Longitude: -125.147300 * Date/Time Start: 2002-08-05T08:30:00 * Date/Time End: 2002-08-06T07:15:00 * Elevation: -777.2 m * Penetration: 90 m * Recovery: 57.37 m * Location: North Pacific Ocean * Campaign: * Basis: Joides Resolution * Method/Device: Drilling/drill rig (DRILL) * Comment: 16 cores; 82.5 m cored; 7.5 m drilled; 69.5 % recovery

204-1250 * Latitude: 44.568600 * Longitude: -125.150183 * Date/Time Start: 2002-07-21T00:00:00 * Date/Time End: 2002-08-27T00:00:00 * Elevation: -795.8 m * Penetration: 876 m * Recovery: 332 m * Location: North Pacific Ocean * Campaign: * Basis: Joides Resolution * Method/Device: Composite Core (COMPCORE) * Comment: 53 cores; 378 m cored; 498 m drilled; 87.8 % recovery

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Size:

5 datasets

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Datasets listed in this publication series

Lin, Y-S; Biddle, JF; Lipp, JS et al. (2010): (Figure 2) Changes of IPLs in subsurface sediments from ODP Leg 204, Hydrate Ridge, under different storage conditions. https://doi.org/10.1594/PANGAEA.745152
Lin, Y-S; Biddle, JF; Lipp, JS et al. (2010): (Figure 3) Changes of IPLs in subsurface sediments from IODP Expedition 311, Cascadia Margin, under different storage conditions. https://doi.org/10.1594/PANGAEA.745154
Lin, Y-S; Biddle, JF; Lipp, JS et al. (2010): (Figure 4) Comparison of microbial communities in subsurface sediment from Sample 1328-152.7 after different storage conditions or incubation with methanol at room temperature. https://doi.org/10.1594/PANGAEA.756693
Lin, Y-S; Biddle, JF; Lipp, JS et al. (2010): (Table 1) Oxygen contents and sulfate concentrations from shipboard and shore-based measurements of some refrigerated samples from IODP Expedition 311 and ODP Leg 204 (after 708 days of storage). https://doi.org/10.1594/PANGAEA.756632
Lin, Y-S; Biddle, JF; Lipp, JS et al. (2010): (Table 2) Quantitative PCR detection of mcrA genes and intact polar archaeol and hydroxyarchaeol in sample 1328-152.7 under different storage conditions and after incubation with methanol at room temperature. https://doi.org/10.1594/PANGAEA.756669