Tan, Tjhing Lok; Rüger, Hans-Jürgen (1999): Oligotrophic bacteria from the Antarctic Ocean [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.745115
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Abstract:
From enrichment cultures in dialysis chambers held in natural seawater tanks, 104 strains were isolated and kept in culture. All strains proved to be Gram-negative and psychrotrophic, having optimum growth temperatures of between 20 and 24 °C. Maximal growth temperatures were 30 to 37 °C, or even higher. With 55 isolates, substrate utilizations in Biolog MicroPlates were determined, and the obtained metabolic fingerprints used for clustering. Five groups could be distinguished at the 80% similarity level. Fifteen strains belonged to cluster 1, seven strains to cluster 2, and each of the clusters 3 and 4 contained nine strains. Cluster 5 can be divided into subcluster 5a and 5b, with 6 strains showing a few substrates metabolized, and 9 strains without any reactions, or weak reactions for one or two substrates, respectively. Each cluster could be characterized by specific metabolic fingerprints. Strains from cluster 1 metabolized N-acetyl-D-glucosamine, alpha-hydroxybutyric acid and gamma-hydroxybutyric acid, strains from cluster 2 citric acid, formic acid, thymidine and putrescine, strains from cluster 3 glycyl-L-aspartic acid, glycyl-L-glutamic acid, L-threonine and inosine, whereas strains from cluster 4 metabolized alpha-cyclodextrin and N-acetyl-D-galactosamine, typically. Methylamine was not utilized by the isolates, but strains from cluster 1, 2 and 3 could grow on basal seawater agar. Morphological characteristics and photomicrographs of the oligotrophic strains are presented. Due to their typical morphologies and ampicillin resistence, the nine strains from cluster 3 can be regarded as new species of the genus Planctomyces. These bacteria have not been cultivated before.
Related to:
Tan, Tjhing Lok; Rüger, Hans-Jürgen (1999): Enrichment, isolation, and Biolog metabolic fingerprints of oligotrophic bacteria from the Antarctic Ocean. Advances in Limnology, 54, 255-272
Coverage:
Median Latitude: -66.459184 * Median Longitude: 27.889713 * South-bound Latitude: -67.735000 * West-bound Longitude: 8.890000 * North-bound Latitude: -64.956700 * East-bound Longitude: 37.460000
Date/Time Start: 1990-03-24T18:30:00 * Date/Time End: 1990-04-15T18:55:00
Event(s):
PS16/507 * Latitude: -66.186700 * Longitude: 35.308300 * Date/Time: 1990-03-24T18:30:00 * Elevation: -4149.0 m * Location: Cosmonauts Sea * Campaign: ANT-VIII/6 (PS16) * Basis: Polarstern * Method/Device: Water sample (WS)
PS16/509 * Latitude: -66.111700 * Longitude: 34.296700 * Date/Time: 1990-03-25T09:48:00 * Elevation: -2950.0 m * Location: Riiser-Larsen Sea * Campaign: ANT-VIII/6 (PS16) * Basis: Polarstern * Method/Device: Water sample (WS)
PS16/518 * Latitude: -64.956700 * Longitude: 33.623300 * Date/Time: 1990-03-28T06:41:00 * Elevation: -2220.0 m * Location: Indian Ocean * Campaign: ANT-VIII/6 (PS16) * Basis: Polarstern * Method/Device: Water sample (WS)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
2 datasets
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Datasets listed in this publication series
- Tan, TL; Rüger, H-J (1999): Origin of oligotrophic strains from enrichment cultures in dialysis chambers (Table 1) and the corresponding Dendrogram (Fig. 1). https://doi.org/10.1594/PANGAEA.745111
- Tan, TL; Rüger, H-J (1999): Table 2. Metabolic fingerprints of Antarctic oligotrophs from cluster 1, 2, 3, 4. https://doi.org/10.1594/PANGAEA.745113