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Tan, Tjhing Lok; Reinke, Manfred; Rüger, Hans-Jürgen (1996): Turbidity measurements in 96-wells microplates to determine the densities of copiotrophic and oligotrophic bacteria with the Most-Probable-Number-method during POLARSTERN cruise ANT-XI/2 [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.745108, Supplement to: Tan, TL et al. (1996): New dilution method in microtiter-plates for enumeration and enrichment of copiotrophic and oligotrophic bacteria. Archiv für Hydrobiologie Supplement, 137(4), 511-521

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Abstract:
A new microtiter-plate dilution method was applied during the expedition ANTARKTIS-XI/2 with RV Polarstern to determine the distribution of copiotrophic and oligotrophic bacteria in the water columns at polar fronts. Twofold serial dilutions were performed with an eight-channel Electrapette in 96-wells plates by mixing 150 µl of seawater with 150 µl of copiotrophic or olitrophic Trypticase-Broth, three times per well. After incubation of about 6 month at 2 °C, turbidities were measured with an eight-channel photometer at 405 nm and combinations of positive test results for three consecutive dilutions chosen and compared with a Most Probable Number table, calculated for 8 replicates and twofold serial dilutions. Densities of 12 to 661 cells/ml for copiotrophs, and 1 to 39 cells/ml for oligotrophs were found. Colony Forming Units on copiotrophic Trypticase-Agar were between 6 and 847 cells/ml, which is in the same range as determined with the MPN method.
Coverage:
Median Latitude: -46.600429 * Median Longitude: -16.142186 * South-bound Latitude: -54.631667 * West-bound Longitude: -33.640000 * North-bound Latitude: -41.275000 * East-bound Longitude: 8.981667
Date/Time Start: 1993-12-15T20:55:00 * Date/Time End: 1994-01-02T13:19:00
Event(s):
PS28/256 (PS2489) * Latitude Start: -42.886667 * Longitude: 8.981667 * Date/Time Start: 1993-12-15T20:55:00 * Date/Time End: 1993-12-16T07:43:00 * Elevation Start: -3792.0 m * Elevation End: -3803.0 m * Campaign: ANT-XI/2 (PS28) * Basis: Polarstern * Method/Device: Multiple investigations (MULT)
PS28/264 (PS2491) * Latitude Start: -44.955000 * Longitude Start: 5.970000 * Latitude End: -44.965000 * Longitude End: 5.970000 * Date/Time Start: 1993-12-17T08:15:00 * Date/Time End: 1993-12-17T19:00:00 * Elevation Start: -4322.0 m * Elevation End: -4324.0 m * Campaign: ANT-XI/2 (PS28) * Basis: Polarstern * Method/Device: Multiple investigations (MULT)
PS28/293 (PS2495) * Latitude Start: -41.288333 * Longitude Start: -14.500000 * Latitude End: -41.275000 * Longitude End: -14.490000 * Date/Time Start: 1993-12-22T06:15:00 * Date/Time End: 1993-12-22T18:10:00 * Elevation Start: -3135.0 m * Elevation End: -3135.0 m * Campaign: ANT-XI/2 (PS28) * Basis: Polarstern * Method/Device: Multiple investigations (MULT)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
14 datasets

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Datasets listed in this publication series

  1. Tan, TL; Reinke, M; Rüger, H-J (1996): Table 1. Densities of copiotrophic and oligotrophic bacteria at station PS28/256. https://doi.org/10.1594/PANGAEA.745094
  2. Tan, TL; Reinke, M; Rüger, H-J (1996): Table 1. Densities of copiotrophic and oligotrophic bacteria at station PS28/264. https://doi.org/10.1594/PANGAEA.745095
  3. Tan, TL; Reinke, M; Rüger, H-J (1996): Table 1. Densities of copiotrophic and oligotrophic bacteria at station PS28/293. https://doi.org/10.1594/PANGAEA.745096
  4. Tan, TL; Reinke, M; Rüger, H-J (1996): Table 2. Turbidities measured in copiotrophic Trypticase-Broth at station PS28/293. https://doi.org/10.1594/PANGAEA.745106
  5. Tan, TL; Reinke, M; Rüger, H-J (1996): Table 3. Turbidities measured in oligotrophic Trypticase-Broth at station PS28/293. https://doi.org/10.1594/PANGAEA.745107
  6. Tan, TL; Reinke, M; Rüger, H-J (1996): Table 1. Densities of copiotrophic and oligotrophic bacteria at station PS28/298. https://doi.org/10.1594/PANGAEA.745097
  7. Tan, TL; Reinke, M; Rüger, H-J (1996): Table 1. Densities of copiotrophic and oligotrophic bacteria at station PS28/301. https://doi.org/10.1594/PANGAEA.745098
  8. Tan, TL; Reinke, M; Rüger, H-J (1996): Table 1. Densities of copiotrophic and oligotrophic bacteria at station PS28/314. https://doi.org/10.1594/PANGAEA.745099
  9. Tan, TL; Reinke, M; Rüger, H-J (1996): Table 1. Densities of copiotrophic and oligotrophic bacteria at station PS28/316. https://doi.org/10.1594/PANGAEA.745100
  10. Tan, TL; Reinke, M; Rüger, H-J (1996): Table 1. Densities of copiotrophic and oligotrophic bacteria at station PS28/329. https://doi.org/10.1594/PANGAEA.745101
  11. Tan, TL; Reinke, M; Rüger, H-J (1996): Table 1. Densities of copiotrophic and oligotrophic bacteria at station PS28/334. https://doi.org/10.1594/PANGAEA.745102
  12. Tan, TL; Reinke, M; Rüger, H-J (1996): Table 1. Densities of copiotrophic and oligotrophic bacteria at station PS28/339. https://doi.org/10.1594/PANGAEA.745103
  13. Tan, TL; Reinke, M; Rüger, H-J (1996): Table 1. Densities of copiotrophic and oligotrophic bacteria at station PS28/358. https://doi.org/10.1594/PANGAEA.745104
  14. Tan, TL; Reinke, M; Rüger, H-J (1996): Table 1. Densities of copiotrophic and oligotrophic bacteria at station PS28/367. https://doi.org/10.1594/PANGAEA.745105