Citation:

bg-7-1109-2010

Name: Seawater carbonate chemistry and processes during experiments with Emiliania huxleyi (2005 Bergen) and Coccolithus braarudii (RCC 1200), 2010
Published: 2010-08-26
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Coccolithus braarudii; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Single species

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Abstract:

The physiological performance of two coccolithophore species,Emiliania huxleyi and Coccolithus braarudii, was investigated during long-term exposure to elevated pCO2 levels. Mono-specific cultures were grown over 152 (E. huxleyi) and 65 (C. braarudii) generations while pCO2 was gradually increased to maximum levels of 1150 ?atm (E. huxleyi) and 930 ?atm (C. braarudii) and kept constant thereafter. Rates of cell growth and cell quotas of particulate organic carbon (POC), particulate inorganic carbon (PIC) and total particulate nitrogen (TPN) were determined repeatedly throughout the incubation period. Increasing pCO2 caused a decrease in cell growth rate of 9% and 29% in E. huxleyi and C. braarudii, respectively. In both species cellular PIC:TPN and PIC:POC ratios decreased in response to rising pCO2, whereas no change was observed in the POC:TPN ratios of E. huxleyi and C. braarudii. These results are consistent with those obtained in shorter-term high CO2exposure experiments following abrupt pertubations of the seawater carbonate system and indicate that for the strains tested here a gradual CO2 increase does not alleviate CO2/pH sensitivity.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Coccolithus braarudii; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Single species

Project(s):

Biological Impacts of Ocean Acidification (BIOACID)

European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)

European Project on Ocean Acidification (EPOCA)

Ocean Acidification International Coordination Centre (OA-ICC)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Sixth Framework Programme (FP6), grant/award no. 511106 : European network of excellence for Ocean Ecosystems Analysis

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Experimental treatment	Exp treat		Müller, Marius N
2	Species	Species		Müller, Marius N
3	Experiment day	Exp day	day	Müller, Marius N
4	Salinity	Sal		Müller, Marius N
5	Temperature, water	Temp	°C	Müller, Marius N
6	Light:Dark cycle	L:D	hh:hh	Müller, Marius N
7	Radiation, photosynthetically active	PAR	µmol/m²/s	Müller, Marius N
8	Nitrite	[NO2]-	µmol/l	Müller, Marius N
9	Phosphate	[PO4]3-	µmol/l	Müller, Marius N
10	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Müller, Marius N		Duplicate I
11	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Müller, Marius N		Duplicate II
12	pH	pH		Müller, Marius N	Metrohm Titrando titrator	Total scale, Duplicate I
13	pH	pH		Müller, Marius N	Metrohm Titrando titrator	Total scale, Duplicate II
14	Growth rate	µ	#/day	Müller, Marius N	Calculated	Duplicate I
15	Growth rate	µ	#/day	Müller, Marius N	Calculated	Duplicate II
16	Carbon, organic/Nitrogen, total ratio	C org/TN		Müller, Marius N	Calculated	Duplicate I
17	Carbon, organic/Nitrogen, total ratio	C org/TN		Müller, Marius N	Calculated	Duplicate II
18	Particulate inorganic carbon/total particulate nitrogen ratio	PIC/TPN		Müller, Marius N	Calculated	Duplicate I
19	Particulate inorganic carbon/total particulate nitrogen ratio	PIC/TPN		Müller, Marius N	Calculated	Duplicate II
20	Particulate inorganic carbon/particulate organic carbon ratio	PIC/POC		Müller, Marius N	Calculated	Duplicate I
21	Particulate inorganic carbon/particulate organic carbon ratio	PIC/POC		Müller, Marius N	Calculated	Duplicate II
22	Carbonate system computation flag	CSC flag		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
23	Carbon dioxide	CO2	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
24	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
25	Bicarbonate ion	[HCO3]-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
26	Carbonate ion	[CO3]2-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
27	Carbon, inorganic, dissolved	DIC	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
28	Alkalinity, total	AT	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
29	Aragonite saturation state	Omega Arg		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
30	Calcite saturation state	Omega Cal		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1405 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Exp treat	2 Species	3 Exp day [day]	4 Sal	5 Temp [°C]	6 L:D [hh:hh]	7 PAR [µmol/m²/s]	8 [NO2]- [µmol/l]	9 [PO4]3- [µmol/l]	10 pCO2water_SST_wet [µatm] (Duplicate I)	11 pCO2water_SST_wet [µatm] (Duplicate II)	12 pH (Total scale, Duplicate I, Met...)	13 pH (Total scale, Duplicate II, Me...)	14 µ [#/day] (Duplicate I, Calculated)	15 µ [#/day] (Duplicate II, Calculated)	16 C org/TN (Duplicate I, Calculated)	17 C org/TN (Duplicate II, Calculated)	18 PIC/TPN (Duplicate I, Calculated)	19 PIC/TPN (Duplicate II, Calculated)	20 PIC/POC (Duplicate I, Calculated)	21 PIC/POC (Duplicate II, Calculated)	22 CSC flag (Calculated using seacarb afte...)	23 CO2 [µmol/kg] (Calculated using seacarb afte...)	24 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	25 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	26 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	27 DIC [µmol/kg] (Calculated using seacarb afte...)	28 AT [µmol/kg] (Calculated using seacarb afte...)	29 Omega Arg (Calculated using seacarb afte...)	30 Omega Cal (Calculated using seacarb afte...)
control	Emiliania huxleyi Bergen 2005	6	33	16	14:10	140	88	3.6	276	264	8.17	8.18	0.83	0.87	9.84	9.87	6.0	7.2	0.71	0.85	21	9.89	269.05	1729.26	192.82	1932.0	2207.2	2.99	4.66
control	Emiliania huxleyi Bergen 2005	12	33	16	14:10	140	88	3.6	264	262	8.23	8.23	0.97	0.88							21	9.63	262.07	1889.96	236.45	2136.0	2463.8	3.66	5.71
control	Emiliania huxleyi Bergen 2005	18	33	16	14:10	140	88	3.6	281	277	8.20	8.21	1.12	1.08							21	10.22	278.02	1914.70	228.76	2153.7	2469.7	3.54	5.53
control	Emiliania huxleyi Bergen 2005	26	33	16	14:10	140	88	3.6	222	228	8.28	8.28	1.06	1.02	9.00	8.99	3.6	4.7	0.46	0.61	21	8.24	224.21	1814.17	254.66	2077.1	2433.6	3.95	6.15
control	Emiliania huxleyi Bergen 2005	34	33	16	14:10	140	88	3.6	254	254	8.23	8.23	1.12	1.09							21	9.30	253.11	1825.28	228.36	2062.9	2383.0	3.54	5.52
control	Emiliania huxleyi Bergen 2005	40	33	16	14:10	140	88	3.6	220	220	8.27	8.28	1.25	1.20	9.05	9.77	5.6	5.5	0.72	0.66	21	8.06	219.23	1773.86	249.00	2030.9	2382.0	3.86	6.02
control	Emiliania huxleyi Bergen 2005	47	33	16	14:10	140	88	3.6	257	247	8.22	8.23	1.00	1.06	10.70	10.22	4.6	5.2	0.50	0.60	21	9.23	251.11	1810.91	226.56	2046.7	2365.0	3.51	5.47
control	Emiliania huxleyi Bergen 2005	53	33	16	14:10	140	88	3.6	276	273	8.20	8.20	1.10	1.09							21	10.05	273.53	1840.94	214.94	2065.9	2366.6	3.33	5.19
control	Emiliania huxleyi Bergen 2005	59	33	16	14:10	140	88	3.6	254	277	8.23	8.20		1.10		12.80		5.9		0.54	21	9.72	264.57	1864.49	227.95	2102.2	2419.6	3.53	5.51
control	Emiliania huxleyi Bergen 2005	66	33	16	14:10	140	88	3.6	281	278	8.20	8.20	1.04	1.04	11.26	11.00	4.7	4.8	0.48	0.50	21	10.24	278.52	1874.47	218.86	2103.6	2407.9	3.39	5.29
control	Emiliania huxleyi Bergen 2005	73	33	16	14:10	140	88	3.6	259	254	8.22	8.22	1.14	1.15	8.64	8.37	5.1	5.3	0.69	0.74	21	9.40	255.60	1801.29	220.22	2030.9	2340.9	3.41	5.32
control	Emiliania huxleyi Bergen 2005	79	33	16	14:10	140	88	3.6	287	288	8.19	8.19	1.13	1.11	11.62		5.7		0.58	0.47	21	10.53	286.49	1884.23	214.99	2109.8	2408.2	3.33	5.19
control	Emiliania huxleyi Bergen 2005	85	33	16	14:10	140	88	3.6	254	255	8.23	8.23	1.11	1.06	10.58	9.13	4.0	3.2	0.44	0.41	21	9.32	253.60	1828.88	228.81	2067.0	2387.4	3.54	5.53
control	Emiliania huxleyi Bergen 2005	91	33	16	14:10	140	88	3.6	256	248	8.23	8.24	1.15	1.13	10.39		3.6		0.41		21	9.23	251.11	1853.09	237.23	2099.6	2430.3	3.68	5.73
control	Emiliania huxleyi Bergen 2005	98	33	16	14:10	140	88	3.6	238	237	8.26	8.26	1.10	1.10	8.19	12.71	3.3	5.2	0.47	0.41	21	8.70	236.66	1828.77	245.16	2082.6	2425.5	3.80	5.92
treatment	Emiliania huxleyi Bergen 2005	6	33	16	14:10	140	88	3.6	313	335	8.15	8.13	0.74	0.71	12.41	11.35	6.9	6.7	0.65	0.69	21	11.87	322.86	1892.52	192.45	2096.8	2363.3	2.98	4.65
treatment	Emiliania huxleyi Bergen 2005	12	33	16	14:10	140	88	3.6	493	498	7.97	7.97	0.93	0.88							21	18.15	493.76	1956.77	134.53	2109.4	2287.9	2.08	3.25
treatment	Emiliania huxleyi Bergen 2005	18	33	16	14:10	140	88	3.6	528	501	7.95	7.97	1.13	1.12							21	18.85	512.69	1985.55	133.40	2137.8	2313.3	2.07	3.22
treatment	Emiliania huxleyi Bergen 2005	26	33	16	14:10	140	88	3.6	409	404	8.04	8.05	1.06	1.08	8.38	7.74	4.5	4.6	0.63	0.69	21	14.89	405.07	1930.00	159.53	2104.4	2321.6	2.47	3.85
treatment	Emiliania huxleyi Bergen 2005	34	33	16	14:10	140	88	3.6	667	667	7.84	7.84	1.12	1.13							21	24.43	664.65	1952.64	99.52	2076.6	2199.5	1.54	2.40
treatment	Emiliania huxleyi Bergen 2005	40	33	16	14:10	140	88	3.6	590	585	7.88	7.88	1.18	1.18					0.80	0.75	21	21.52	585.43	1885.84	105.39	2012.7	2148.5	1.63	2.55
treatment	Emiliania huxleyi Bergen 2005	47	33	16	14:10	140	88	3.6	880	906	7.71	7.70	1.05	0.98	9.37	11.84	4.3	5.1	0.53	0.50	21	32.71	889.86	1937.97	73.22	2043.9	2121.0	1.13	1.77
treatment	Emiliania huxleyi Bergen 2005	53	33	16	14:10	140	88	3.6	1041	1019	7.66	7.67	1.00	1.03							21	37.73	1026.38	2038.61	70.25	2146.6	2212.7	1.09	1.70
treatment	Emiliania huxleyi Bergen 2005	59	33	16	14:10	140	88	3.6	1222	1275	7.59	7.57	0.97	0.93	7.97	7.61					21	45.73	1244.11	2008.56	56.26	2110.6	2148.8	0.87	1.36
treatment	Emiliania huxleyi Bergen 2005	66	33	16	14:10	140	88	3.6	1249	1224	7.58	7.58	0.98	0.97	9.14	11.57	3.6	3.4	0.46	0.35	21	45.29	1232.15	1989.26	55.72	2090.3	2128.4	0.86	1.35
treatment	Emiliania huxleyi Bergen 2005	73	33	16	14:10	140	88	3.6	1258	1288	7.57	7.56	1.00	0.98	9.97	9.92	3.5	4.2	0.41	0.50	21	46.63	1268.52	2001.36	54.78	2102.8	2138.0	0.85	1.32
treatment	Emiliania huxleyi Bergen 2005	80	33	16	14:10	140	88	3.6	878	855	7.72	7.73	1.13	1.14	11.57	9.79	3.5	4.5	0.36	0.54	21	31.74	863.45	1969.09	77.90	2078.7	2163.0	1.21	1.88
treatment	Emiliania huxleyi Bergen 2005	86	33	16	14:10	140	88	3.6	1146	1151	7.61	7.61	0.94	1.01	9.08	10.45	1.4	1.5	0.18	0.16	21	42.07	1144.46	1979.83	59.42	2081.3	2128.2	0.92	1.44
treatment	Emiliania huxleyi Bergen 2005	92	33	16	14:10	140	88	3.6	1150	1155	7.61	7.61	1.04	1.03		11.08		1.9		0.20	21	42.21	1148.44	1986.72	59.62	2088.6	2135.5	0.92	1.44
treatment	Emiliania huxleyi Bergen 2005	98	33	16	14:10	140	88	3.6	1299	1233	7.57	7.59	0.93	1.00	9.03	10.54	1.8	2.3	0.24	0.25	21	46.37	1261.54	2036.71	57.04	2140.1	2178.5	0.88	1.38
control	Coccolithus braarudii RCC 1200	9	33	16	14:10	140	88	3.6	233	233	8.24	8.24	0.64		10.15	10.28	9.0	9.3	1.03	1.05	21	8.53	232.18	1713.37	219.35	1941.3	2254.8	3.40	5.30
control	Coccolithus braarudii RCC 1200	18	33	16	14:10	140	88	3.6	257	233	8.20	8.24	0.68	0.68	11.13	11.62	10.0	10.4	1.05	1.04	21	8.97	244.14	1720.53	210.35	1939.9	2240.4	3.26	5.08
control	Coccolithus braarudii RCC 1200	24	33	16	14:10	140	88	3.6	275	273	8.21	8.20	0.70	0.71	10.60	10.66	9.8	10.7	1.07	1.17	21	10.04	273.04	1880.39	224.66	2115.1	2427.2	3.48	5.43
control	Coccolithus braarudii RCC 1200	31	33	16	14:10	140	88	3.6	287	287	8.18	8.18	0.69	0.71	10.46	10.26	5.5	6.8	0.61	0.77	21	10.51	285.99	1838.14	204.96	2053.6	2340.4	3.18	4.95
control	Coccolithus braarudii RCC 1200	37	33	16	14:10	140	88	3.6	304	290	8.15	8.16	0.67	0.71	10.76	9.13	7.9	8.6	0.86	1.10	21	10.88	295.95	1816.57	193.43	2020.9	2292.5	3.00	4.67
control	Coccolithus braarudii RCC 1200	42	33	16	14:10	140	88	3.6	297	291	8.16	8.17	0.65	0.67	10.89	9.45	8.5	8.8	0.91	1.09	21	10.77	292.97	1840.11	200.51	2051.4	2331.8	3.11	4.84
control	Coccolithus braarudii RCC 1200	48	33	16	14:10	140	88	3.6	235	256	8.24	8.20	0.73	0.74	8.30	14.19	6.6	9.6	0.93	0.79	21	8.99	244.64	1724.04	210.78	1943.8	2244.8	3.27	5.09
control	Coccolithus braarudii RCC 1200	53	33	16	14:10	140	88	3.6	221	254	8.26	8.23	0.71	0.75							21	8.70	236.66	1787.15	234.12	2030.0	2360.0	3.63	5.66
control	Coccolithus braarudii RCC 1200	59	33	16	14:10	140	88	3.6	261	257	8.18	8.21	0.73		10.71	11.98	8.1	9.9	0.88	0.97	21	9.49	258.09	1736.99	202.80	1949.3	2238.3	3.14	4.90
control	Coccolithus braarudii RCC 1200	65	33	16	14:10	140	88	3.6	267	240	8.18	8.22	0.71	0.57		13.89		11.4	0.71	0.96	21	9.29	252.61	1700.10	198.50	1907.9	2192.8	3.08	4.80
treatment	Coccolithus braarudii RCC 1200	9	33	16	14:10	140	88	3.6	233	233	8.24	8.24	0.65	0.66	10.05	9.56	9.1	9.0	1.06	1.09	21	8.53	232.18	1713.37	219.35	1941.3	2254.8	3.40	5.30
treatment	Coccolithus braarudii RCC 1200	18	33	16	14:10	140	88	3.6	369	365	8.08	8.08	0.68	0.65							21	13.44	365.71	1867.08	165.37	2045.9	2274.6	2.56	4.00
treatment	Coccolithus braarudii RCC 1200	24	33	16	14:10	140	88	3.6	532	540	7.94	7.94	0.67	0.66	7.74	9.74	8.6	7.5	1.29	0.90	21	19.63	534.11	1975.43	126.75	2121.8	2287.5	1.96	3.06
treatment	Coccolithus braarudii RCC 1200	31	33	16	14:10	140	88	3.6	840	840	7.74	7.74	0.57	0.61							21	30.77	837.04	1953.33	79.08	2063.2	2150.4	1.23	1.91
treatment	Coccolithus braarudii RCC 1200	38	33	16	14:10	140	88	3.6	867	801	7.72	7.76	0.53	0.51	10.41	9.34	2.2	1.3	0.24	0.16	21	30.55	831.06	1939.38	78.51	2048.4	2135.3	1.22	1.90
treatment	Coccolithus braarudii RCC 1200	45	33	16	14:10	140	88	3.6	645	794	7.86	7.76	0.48	0.43	7.01	12.19	2.9	1.2	0.48	0.11	21	26.35	716.97	1965.75	93.50	2085.6	2197.7	1.45	2.26
treatment	Coccolithus braarudii RCC 1200	53	33	16	14:10	140	88	3.6	683	975	7.82	7.67	0.49	0.43	7.52	12.11	2.2	1.9	0.34	0.18	21	30.37	826.08	1972.65	81.72	2084.7	2175.8	1.27	1.97
treatment	Coccolithus braarudii RCC 1200	59	33	16	14:10	140	88	3.6	1005	1014	7.66	7.65	0.45						0.28	0.22	21	36.98	1005.95	1952.55	65.75	2055.3	2116.9	1.02	1.59
treatment	Coccolithus braarudii RCC 1200	66	33	16	14:10	140	88	3.6	1051	1221	7.63	7.58	0.51	0.49	8.97	9.54	2.8	2.6	0.36	0.32	21	41.61	1132.00	1958.28	58.77	2058.7	2105.4	0.91	1.42
treatment	Coccolithus braarudii RCC 1200	70	33	16	14:10	140	88	3.6	1051	1221	7.63	7.58	0.43	0.39	6.46	8.96	2.2	1.8	0.39	0.23	21	41.61	1132.00	1958.28	58.77	2058.7	2105.4	0.91	1.42