Citation:

bg-6-2637-2009

Name: Seawater carbonate chemistry and biological processes during experiments with four strains of Emiliania huxleyi
Published: 2009
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species

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Published: 2009 (exact date unknown) • DOI registered: 2010-03-18

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Abstract:

Four strains of the coccolithophore E. huxleyi (RCC1212, RCC1216, RCC1238, RCC1256) were grown in dilute batch culture at four CO2 levels ranging from ~200 µatm to ~1200 µatm. Growth rate, particulate organic carbon content, and particulate inorganic carbon content were measured, and organic and inorganic carbon production calculated. The four strains did not show a uniform response to carbonate chemistry changes in any of the analysed parameters and none of the four strains displayed a response pattern previously described for this species. We conclude that the sensitivity of different strains of E. huxleyi to acidification differs substantially and that this likely has a genetic basis. We propose that this can explain apparently contradictory results reported in the literature.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4 [webpage]. https://cran.r-project.org/package=seacarb

Project(s):

European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)

European Project on Ocean Acidification (EPOCA)

Ocean Acidification International Coordination Centre (OA-ICC)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Sixth Framework Programme (FP6), grant/award no. 511106 : European network of excellence for Ocean Ecosystems Analysis

Coverage:

Median Latitude: 5.162500 * Median Longitude: 76.658333 * South-bound Latitude: -42.300000 * West-bound Longitude: -20.333330 * North-bound Latitude: 63.400000 * East-bound Longitude: 169.833330

Date/Time Start: 1998-09-01T00:00:00 * Date/Time End: 2005-11-30T00:00:00

Event(s):

North_Atlantic_OA * Latitude: 63.400000 * Longitude: -20.333330 * Date/Time Start: 1999-07-01T00:00:00 * Date/Time End: 1999-07-31T00:00:00 * Method/Device: Experiment (EXP) * Comment: Isolation date and location of culture strains

North_Pacific_OA * Latitude: 34.016670 * Longitude: 139.833330 * Date/Time Start: 2005-11-01T00:00:00 * Date/Time End: 2005-11-30T00:00:00 * Method/Device: Experiment (EXP) * Comment: Isolation date and location of culture strains

South_Atlantic_OA * Latitude: -34.466670 * Longitude: 17.300000 * Date/Time Start: 2000-09-01T00:00:00 * Date/Time End: 2000-09-30T00:00:00 * Method/Device: Experiment (EXP) * Comment: Isolation date and location of culture strains

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event
2	Identification	ID		Langer, Gerald
3	Carbonate system computation flag	CSC flag		Langer, Gerald
4	Salinity	Sal		Langer, Gerald	Conductivity meter (WTW, Weilheim, Gemany)
5	Temperature, water	Temp	°C	Langer, Gerald
6	Radiation, photosynthetically active	PAR	µmol/m²/s	Langer, Gerald
7	Nitrate	[NO3]-	µmol/l	Langer, Gerald
8	Phosphate	[PO4]3-	µmol/l	Langer, Gerald
9	Alkalinity, total	AT	µmol/kg	Langer, Gerald	Alkalinity, Gran titration (Gran, 1950)
10	Carbon, inorganic, dissolved	DIC	µmol/kg	Langer, Gerald	Photometry
11	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Langer, Gerald	Calculated
12	pH, NBS scale	pH NBS		Langer, Gerald	Calculated using CO2SYS	NBS scale
13	Carbonate system computation flag	CSC flag		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
14	pH, total scale	pHT		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	total scale
15	Bicarbonate ion	[HCO3]-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
16	Carbonate ion	[CO3]2-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
17	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
18	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
19	Aragonite saturation state	Omega Arg		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
20	Calcite saturation state	Omega Cal		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
21	Growth rate	µ	#/day	Langer, Gerald
22	Growth rate, standard deviation	µ std dev	±	Langer, Gerald
23	Carbon, inorganic, particulate, per cell	PIC/cell	pmol/#	Langer, Gerald	Element analyser CNS, EURO EA
24	Carbon, inorganic, particulate, per cell, standard deviation	PIC/cell std dev	±	Langer, Gerald	Element analyser CNS, EURO EA
25	Carbon, organic, particulate, per cell	POC/cell	pmol/#	Langer, Gerald	Element analyser CNS, EURO EA
26	Carbon, organic, particulate, per cell, standard deviation	POC/cell std dev	±	Langer, Gerald	Element analyser CNS, EURO EA
27	Carbon, inorganic, particulate, production per cell	PIC prod/cell	pmol/#/day	Langer, Gerald	Element analyser CNS, EURO EA
28	Carbon, inorganic, particulate, production, standard deviation	PIC prod std dev	±	Langer, Gerald	Element analyser CNS, EURO EA
29	Carbon, organic, particulate, production per cell	POC prod/cell	pmol/#/day	Langer, Gerald	Element analyser CNS, EURO EA
30	Carbon, organic, particulate, production, standard deviation	POC prod std dev	±	Langer, Gerald	Element analyser CNS, EURO EA
31	Particulate inorganic carbon/particulate organic carbon ratio	PIC/POC		Langer, Gerald
32	Particulate inorganic carbon/particulate organic carbon ratio, standard deviation	PIC/POC ratio std dev	±	Langer, Gerald

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

496 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Event	2 ID	3 CSC flag	4 Sal (Conductivity meter (WTW, Weil...)	5 Temp [°C]	6 PAR [µmol/m²/s]	7 [NO3]- [µmol/l]	8 [PO4]3- [µmol/l]	9 AT [µmol/kg] (Alkalinity, Gran titration (G...)	10 DIC [µmol/kg] (Photometry)	11 pCO2water_SST_wet [µatm] (Calculated)	12 pH NBS (NBS scale, Calculated using C...)	13 CSC flag (Calculated using seacarb afte...)	14 pHT (total scale, Calculated using...)	15 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	16 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	17 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	18 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	19 Omega Arg (Calculated using seacarb afte...)	20 Omega Cal (Calculated using seacarb afte...)	21 µ [#/day]	22 µ std dev [±]	23 PIC/cell [pmol/#] (Element analyser CNS, EURO EA)	24 PIC/cell std dev [±] (Element analyser CNS, EURO EA)	25 POC/cell [pmol/#] (Element analyser CNS, EURO EA)	26 POC/cell std dev [±] (Element analyser CNS, EURO EA)	27 PIC prod/cell [pmol/#/day] (Element analyser CNS, EURO EA)	28 PIC prod std dev [±] (Element analyser CNS, EURO EA)	29 POC prod/cell [pmol/#/day] (Element analyser CNS, EURO EA)	30 POC prod std dev [±] (Element analyser CNS, EURO EA)	31 PIC/POC	32 PIC/POC ratio std dev [±]
North_Pacific_OA	RCC1238-1	15	32	20	400	100	6.25	2522	2086	206	8.45	15	8.32	1768	311	205.28	204.59	4.90	7.6	1.48	0.07	0.734	0.064	0.867	0.062	1.092	0.145	1.289	0.150	0.85	0.01
North_Pacific_OA	RCC1238-2	15	32	20	400	100	6.25	2302	2050	395	8.19	15	8.06	1858	179	392.70	391.38	2.83	4.4	1.64	0.01	0.691	0.026	0.868	0.050	1.131	0.043	1.420	0.083	0.80	0.02
North_Pacific_OA	RCC1238-3	15	32	20	400	100	6.25	2184	2039	681	7.96	15	7.84	1907	110	675.39	673.13	1.73	2.7	1.67	0.02	0.642	0.048	0.965	0.068	1.074	0.092	1.616	0.127	0.66	0.02
North_Pacific_OA	RCC1238-4	15	32	20	400	100	6.25	2107	2013	929	7.83	15	7.70	1902	80	925.73	922.63	1.26	2.0	1.60	0.02	0.702	0.022	0.799	0.067	1.123	0.043	1.278	0.103	0.88	0.08
Tasman_Sea_OA	RCC1216-1	15	32	17	400	100	6.25	2487	2102	218	8.42	15	8.30	1821	273	216.80	216.04	4.26	6.7	1.14	0.01	0.889	0.063	0.875	0.078	1.011	0.072	0.995	0.090	1.02	0.02
Tasman_Sea_OA	RCC1216-2	15	32	17	400	100	6.25	2281	2067	422	8.16	15	8.03	1898	153	420.08	418.62	2.39	3.7	1.14	0.05	0.890	0.065	0.956	0.073	1.004	0.098	1.078	0.111	0.93	0.01
Tasman_Sea_OA	RCC1216-3	15	32	17	400	100	6.25	2177	2060	729	7.93	15	7.81	1941	93	725.97	723.45	1.45	2.3	1.11	0.00	0.833	0.081	1.028	0.048	0.923	0.093	1.139	0.055	0.81	0.04
Tasman_Sea_OA	RCC1216-4	15	32	17	400	100	6.25	2075	2029	1201	7.71	15	7.58	1930	56	1202.70	1198.52	0.86	1.4	1.01	0.02	0.713	0.103	1.075	0.009	0.723	0.096	1.091	0.013	0.66	0.09
North_Atlantic_OA	RCC1256-1	15	32	17	400	100	6.25	2456	2049	193	8.46	15	8.34	1756	286	191.72	191.05	4.48	7.0	1.28	0.01	0.804	0.090	1.032	0.087	1.033	0.123	1.323	0.103	0.78	0.10
North_Atlantic_OA	RCC1256-2	15	32	17	400	100	6.25	2240	2022	399	8.17	15	8.05	1853	155	396.95	395.57	2.41	3.8	1.28	0.01	0.910	0.053	1.112	0.083	1.163	0.069	1.419	0.109	0.82	0.01
North_Atlantic_OA	RCC1256-3	15	32	17	400	100	6.25	2136	1993	587	8.00	15	7.88	1865	106	585.36	583.32	1.66	2.6	1.14	0.03	1.006	0.096	1.381	0.128	1.196	0.075	1.572	0.138	0.73	0.00
North_Atlantic_OA	RCC1256-4	15	32	17	400	100	6.25	2051	1974	915	7.81	15	7.69	1872	69	911.05	907.89	1.07	1.7	0.83	0.01	1.019	0.001	1.415	0.048	0.847	0.008	1.177	0.038	0.72	0.03
South_Atlantic_OA	RCC1212-1	15	32	20	400	100	6.25	2517	2067	194	8.47	15	8.34	1741	319	192.96	192.32	5.05	7.8	0.99	0.02	0.801	0.018	0.907	0.071	0.793	0.003	0.898	0.058	0.89	0.06
South_Atlantic_OA	RCC1212-2	15	32	20	400	100	6.25	2313	2066	409	8.18	15	8.05	1877	176	407.00	405.64	2.78	4.3	0.98	0.01	0.779	0.043	0.951	0.093	0.765	0.046	0.934	0.095	0.82	0.04
South_Atlantic_OA	RCC1212-3	15	32	20	400	100	6.25	2203	2071	752	7.93	15	7.80	1944	103	748.63	746.12	1.62	2.5	0.95	0.03	0.738	0.038	1.058	0.089	0.699	0.058	1.002	0.116	0.70	0.02
South_Atlantic_OA	RCC1212-4	15	32	20	400	100	6.25	2128	2053	1096	7.77	15	7.64	1947	71	1090.22	1086.57	1.12	1.7	0.87	0.02	0.571	0.120	0.998	0.071	0.493	0.096	0.864	0.078	0.58	0.14