Not logged in
PANGAEA.
Data Publisher for Earth & Environmental Science

Hauton, C; Tyrrell, Toby; Williams, J (2009): Seawater carbonate chemistry and processes during experiments with amphipod Gammarus locusta, 2009 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.728727, Supplement to: Hauton, C et al. (2009): The subtle effects of sea water acidification on the amphipod Gammarus locusta. Biogeosciences, 6(8), 1479-1489, https://doi.org/10.5194/bg-6-1479-2009

Always quote citation above when using data! You can download the citation in several formats below.

RIS CitationBibTeX Citation

Abstract:
We report an investigation of the effects of increases in pCO2 on the survival, growth and molecular physiology of the neritic amphipod Gammarus locusta which has a cosmopolitan distribution in estuaries. Amphipods were reared from juvenile to mature adult in laboratory microcosms at three different levels of pH in nominal range 8.1-7.6. Growth rate was estimated from weekly measures of body length. At sexual maturity the amphipods were sacrificed and assayed for changes in the expression of genes coding for a heat shock protein (hsp70 gene) and the metabolic enzyme glyceraldehyde-3-phosphate dehydrogenase (gapdh gene). The data show that the growth and survival of this species is not significantly impacted by a decrease in sea water pH of up to 0.5 units. Quantitative real-time PCR analysis indicated that there was no significant effect of growth in acidified sea water on the sustained expression of the hsp70 gene. There was a consistent and significant increase in the expression of the gapdh gene at a pH of ~7.5 which, when combined with observations from other workers, suggests that metabolic changes may occur in response to acidification. It is concluded that sensitive assays of tissue physiology and molecular biology should be routinely employed in future studies of the impacts of sea water acidification as subtle effects on the physiology and metabolism of coastal marine species may be overlooked in conventional gross "end-point" studies of organism growth or mortality.
Keyword(s):
Animalia; Arthropoda; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Gammarus locusta; Laboratory experiment; Mortality/Survival; North Atlantic; Single species; Temperate
Project(s):
European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)
European Project on Ocean Acidification (EPOCA)
Ocean Acidification International Coordination Centre (OA-ICC)
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Event(s):
Hauton_etal_09
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 Experiment dayExp daydayHauton, C
2 SalinitySalHauton, CConductivity meter (WTW, Weilheim, Gemany)
3 Temperature, waterTemp°CHauton, CConductivity meter (WTW, Weilheim, Gemany)
4 Light:Dark cycleL:Dhh:hhHauton, CMeasured
5 Carbonate system computation flagCSC flagHauton, CCalculated using seacarb after Nisumaa et al. (2010)
6 pHpHHauton, CSevenMulti pH meter (Mettler, Schwerzenbach, Switzerland)Scale not given
7 pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
8 Alkalinity, totalATµmol/kgHauton, CVINIDTA 3C instrument (Miranda, Kiel, Germany)
9 Carbon, inorganic, dissolvedDICµmol/kgHauton, CVINIDTA 3C instrument (Miranda, Kiel, Germany)
10 Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
11 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)water at sea surface temperature
12 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)water at sea surface temperature
13 Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
14 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
15 Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
16 Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
17 Gammarus locusta, survivalG. loc survival#Hauton, CBatch C
18 Gammarus locusta, survivalG. loc survival#Hauton, CBatch D
19 Gammarus locusta, survivalG. loc survival#Hauton, CBatch E
20 Gammarus locusta, survivalG. loc survival#Hauton, CBatch F
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
444 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Exp day [day]		2 
Sal
(Conductivity meter (WTW, Weil...)		3 
Temp [°C]
(Conductivity meter (WTW, Weil...)		4 
L:D [hh:hh]
(Measured)		5 
CSC flag
(Calculated using seacarb afte...)		6 
pH
(Scale not given, SevenMulti p...)		7 
pH
(Total scale, Calculated using...)		8 
AT [µmol/kg]
(VINIDTA 3C instrument (Mirand...)		9 
DIC [µmol/kg]
(VINIDTA 3C instrument (Mirand...)		10 
CO2 [µmol/kg]
(Calculated using seacarb afte...)		11 
pCO2water_SST_wet [µatm]
(water at sea surface temperat...)		12 
fCO2water_SST_wet [µatm]
(water at sea surface temperat...)		13 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)		14 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)		15 
Omega Arg
(Calculated using seacarb afte...)		16 
Omega Cal
(Calculated using seacarb afte...)		17 
G. loc survival [#]
(Batch C)		18 
G. loc survival [#]
(Batch D)		19 
G. loc survival [#]
(Batch E)		20 
G. loc survival [#]
(Batch F)
033.8520.2312:12158.18.432860.22273.85.37166.3165.81835.44432.976.7810.4422262334
433.8520.2312:12158.18.432860.22273.85.37166.3165.81835.44432.976.7810.441733
533.8520.2312:12158.18.432860.22273.85.37166.3165.81835.44432.976.7810.442225
1133.8520.2312:12158.18.432860.22273.85.37166.3165.81835.44432.976.7810.4422211026
1433.8520.2312:12158.18.432860.22273.85.37166.3165.81835.44432.976.7810.442220
1833.8520.2312:12158.18.432860.22273.85.37166.3165.81835.44432.976.7810.441022
2533.8520.2312:12158.18.432860.22273.85.37166.3165.81835.44432.976.7810.44720
2833.8520.2312:12158.18.432860.22273.85.37166.3165.81835.44432.976.7810.44818
033.6420.0312:12157.88.312862.12383.67.84241.2240.42019.17356.585.588.6125263531
433.6420.0312:12157.88.312862.12383.67.84241.2240.42019.17356.585.588.612825
533.6420.0312:12157.88.312862.12383.67.84241.2240.42019.17356.585.588.612322
1133.6420.0312:12157.88.312862.12383.67.84241.2240.42019.17356.585.588.6123192723
1433.6420.0312:12157.88.312862.12383.67.84241.2240.42019.17356.585.588.612218
1833.6420.0312:12157.88.312862.12383.67.84241.2240.42019.17356.585.588.612423
2533.6420.0312:12157.88.312862.12383.67.84241.2240.42019.17356.585.588.612220
2833.6420.0312:12157.88.312862.12383.67.84241.2240.42019.17356.585.588.612220
033.8520.1412:12157.68.032886.12599.417.18530.7529.02358.99223.223.495.3822262634
433.8520.1412:12157.68.032886.12599.417.18530.7529.02358.99223.223.495.382025
533.8520.1412:12157.68.032886.12599.417.18530.7529.02358.99223.223.495.381726
1133.8520.1412:12157.68.032886.12599.417.18530.7529.02358.99223.223.495.3816251623
1433.8520.1412:12157.68.032886.12599.417.18530.7529.02358.99223.223.495.381622
1833.8520.1412:12157.68.032886.12599.417.18530.7529.02358.99223.223.495.381319
2533.8520.1412:12157.68.032886.12599.417.18530.7529.02358.99223.223.495.381316
2833.8520.1412:12157.68.032886.12599.417.18530.7529.02358.99223.223.495.381312