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Riebesell, Ulf; Zondervan, Ingrid; Rost, Björn; Tortell, Philippe Daniel; Zeebe, Richard E; Morel, Francois M M (2000): Seawater carbonate chemistry and processes during experiments with coccolithophores (Emiliania huxleyi and Gephyrocapsa oceanica), 2000 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.728092, Supplement to: Riebesell, U et al. (2000): Reduced calcification of marine plankton in response to increased atmospheric CO2. Nature, 407, 364-367, https://doi.org/10.1038/35030078

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Abstract:
The formation of calcareous skeletons by marine planktonic organisms and their subsequent sinking to depth generates a continuous rain of calcium carbonate to the deep ocean and underlying sediments1. This is important in regulating marine carbon cycling and ocean-atmosphere CO2 exchange2. The present rise in atmospheric CO2 levels3 causes significant changes in surface ocean pH and carbonate chemistry4. Such changes have been shown to slow down calcification in corals and coralline macroalgae5,6, but the majority of marine calcification occurs in planktonic organisms. Here we report reduced calcite production at increased CO2 concentrations in monospecific cultures of two dominant marine calcifying phytoplankton species, the coccolithophorids Emiliania huxleyi and Gephyrocapsa oceanica . This was accompanied by an increased proportion of malformed coccoliths and incomplete coccospheres. Diminished calcification led to a reduction in the ratio of calcite precipitation to organic matter production. Similar results were obtained in incubations of natural plankton assemblages from the north Pacific ocean when exposed to experimentally elevated CO2 levels. We suggest that the progressive increase in atmospheric CO2 concentrations may therefore slow down the production of calcium carbonate in the surface ocean. As the process of calcification releases CO2 to the atmosphere, the response observed here could potentially act as a negative feedback on atmospheric CO2 levels.
Keyword(s):
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Entire community; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Light; Not applicable; Open ocean; Other studied parameter or process; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Temperate
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Event(s):
Riebesell_etal_2000a * Method/Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
Sample IDSample IDRiebesell, Ulf
Radiation, photosynthetically activePARµmol/m2/sRiebesell, Ulf
Temperature, waterTemp°CRiebesell, UlfCTD, Sea-Bird SBE 911plus
Carbon dioxide, totalTCO2µmol/lRiebesell, Ulf
pHpHRiebesell, Ulf
Coccolithophoridae, totalCoccolith#/mlRiebesell, UlfScanning electron microscope (SEM)
Aphrodite aculeataA. aculeata#Riebesell, UlfCounting
Phytoplankton cell division ratediv#/dayRiebesell, UlfMeasured
Carbon dioxide, dissolvedCO2 dissµmol/lRiebesell, Ulf
10 Carbon dioxide, dissolvedCO2 dissµmol/lRiebesell, Ulf
11 Carbon dioxide, dissolvedCO2 dissµmol/lRiebesell, Ulf
12 Carbon dioxide, dissolvedCO2 dissµmol/lRiebesell, Ulf
13 Alkalinity, totalATmmol(eq)/lRiebesell, UlfAlkalinity, potentiometric
14 Alkalinity, totalATmmol(eq)/lRiebesell, UlfAlkalinity, potentiometric
15 Alkalinity, totalATmmol(eq)/lRiebesell, UlfAlkalinity, potentiometric
16 Alkalinity, totalATmmol(eq)/lRiebesell, UlfAlkalinity, potentiometric
17 Carbon dioxide, totalTCO2µmol/lRiebesell, Ulf
18 Carbon dioxide, totalTCO2µmol/lRiebesell, Ulf
19 Carbon dioxide, totalTCO2µmol/lRiebesell, Ulf
20 pHpHRiebesell, Ulf
21 pHpHRiebesell, Ulf
22 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmRiebesell, Ulf
23 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmRiebesell, Ulf
24 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
25 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
26 Carbon, total, particulateTPC%Riebesell, Ulf
27 Total particulate carbon production per cellTPC prod/cellpmol/#/dayRiebesell, UlfMeasured
28 Particulate organic carbon production per cellPOC prod/cellpmol/#/dayRiebesell, UlfMeasured
29 Particulate inorganic carbon production per cellPIC prod/cellpmol/#/dayRiebesell, Ulf
30 δ13C, total particulate carbonδ13C TPC‰ PDBRiebesell, UlfMeasured
31 δ13C, particulate organic carbonδ13C POC‰ PDBRiebesell, Ulf
32 δ13C, particulate inorganic carbonδ13C PICRiebesell, Ulf
33 δ13C, dissolved inorganic carbonδ13C DIC‰ PDBRiebesell, UlfMass spectrometer Finnigan Delta-S
34 δ13C, carbon dioxide, aquaticδ13C CO2 aqRiebesell, Ulf
35 Isotopic fractionation, during photosynthisεpRiebesell, UlfCalculated after Freeman & Hayes (1992)
36 Isotopic fractionation, during photosynthisεpRiebesell, UlfCalculated after Freeman & Hayes (1992)
37 Carbon/Nitrogen ratioC/NRiebesell, UlfCalculated, see reference(s)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
3006 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Sample ID

PAR [µmol/m2/s]

Temp [°C]
(CTD, Sea-Bird SBE 911plus)

TCO2 [µmol/l]

pH

Coccolith [#/ml]
(Scanning electron microscope ...)

A. aculeata [#]
(Counting)

div [#/day]
(Measured)

CO2 diss [µmol/l]
10 
CO2 diss [µmol/l]
11 
CO2 diss [µmol/l]
12 
CO2 diss [µmol/l]
13 
AT [mmol(eq)/l]
(Alkalinity, potentiometric)
14 
AT [mmol(eq)/l]
(Alkalinity, potentiometric)
15 
AT [mmol(eq)/l]
(Alkalinity, potentiometric)
16 
AT [mmol(eq)/l]
(Alkalinity, potentiometric)
17 
TCO2 [µmol/l]
18 
TCO2 [µmol/l]
19 
TCO2 [µmol/l]
20 
pH
21 
pH
22 
pCO2water_SST_wet [µatm]
23 
pCO2water_SST_wet [µatm]
24 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)
25 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)
26 
TPC [%]
27 
TPC prod/cell [pmol/#/day]
(Measured)
28 
POC prod/cell [pmol/#/day]
(Measured)
29 
PIC prod/cell [pmol/#/day]
30 
δ13C TPC [‰ PDB]
(Measured)
31 
δ13C POC [‰ PDB]
32 
δ13C PIC []
33 
δ13C DIC [‰ PDB]
(Mass spectrometer Finnigan De...)
34 
δ13C CO2 aq []
35 
εp []
(Calculated after Freeman & Ha...)
36 
εp []
(Calculated after Freeman & Ha...)
37 
C/N
(Calculated, see reference(s))
7.650 A15.00015.00037.57.7928251.010.7822116.302117.582087.572086.942.1832.1902.1852.18435.337.530.77.797.87959.000785.00072.57085.41020.60.7830.5820.20047406-18.562-24.74-1.730.190-9.8315.201.925.7
7.650 A15.00015.0002118.852086.302.1972.18339.021.00.7990.5860.21259521-18.777-24.580.190-9.83
7.650 B15.00015.00028441.010.7832088.112087.292.1882.18539.830.67.87784.00085.61021.10.7890.5910.19757843-18.415-24.942.470.190-9.8315.49-2.285.8
7.650 B15.00015.0002086.462.18236.020.90.755-18.5130.190-9.83
7.650 C15.00015.00025698.010.7632087.312086.772.1952.19328.87.90737.00090.72020.00.8350.6520.18342488-18.415-24.45-0.170.190-9.8314.910.365.8
7.650 C15.00015.0002086.222.19220.30.8470.6100.23715655-18.244-24.310.190-9.83
7.820 A15.00015.00032.37.8616373.010.7752153.162152.202137.872137.222.2482.2482.2472.24932.332.328.87.867.91826.000738.00086.45094.83017.50.8150.5930.22177338-19.023-24.95-5.150.190-9.8315.445.365.8
7.820 A15.00015.0002151.232136.572.2492.25032.79.80.8670.5970.27009089-19.209-24.830.190-9.83
7.820 B15.00015.00028596.010.7932122.542122.5832.220.20.7580.5200.2372586-18.570-25.01-3.260.190-9.8315.513.465.7
7.820 B15.00015.0002122.6131.919.40.7260.5180.2080945-18.312-24.900.190-9.83
7.820 C15.00015.00026902.010.7832128.672128.332.2412.24228.37.91724.00095.85018.70.7000.5200.18020738-18.402-25.151.990.190-9.8315.63-1.795.9
7.820 C15.00015.0002127.982.24219.20.7180.5510.16615404-18.513-24.980.190-9.83
7.970 A15.00015.00020.08.0628866.010.7682124.372123.282106.152105.662.2932.2932.2782.28020.220.019.48.068.07513.000495.000130.940132.97020.50.7610.5360.22528142-18.380-24.83-3.470.190-9.8315.233.675.8
7.970 A15.00015.0002122.192105.172.2942.28220.120.80.7720.5410.23140408-18.349-24.540.190-9.83
7.970 B15.00015.00026360.010.7522104.402104.792.2902.29219.918.08.10462.000141.52019.80.8040.5600.24397812-18.431-24.87-3.470.190-9.8315.273.675.7
7.970 B15.00015.0002105.182.29420.019.80.8060.5670.23873998-18.284-24.590.190-9.83
7.970 C15.00015.00027312.010.7562106.982106.362.2962.29517.98.10457.000142.86022.30.8800.5830.29674297-19.172-25.01-5.780.190-9.8315.416.005.9
7.970 C15.00015.0002105.742.29520.80.8190.5790.24012151-18.482-24.700.190-9.83
8.040 A15.00015.00021.08.0423048.010.7762127.402128.662112.922112.842.2902.2912.2992.29521.021.018.68.048.08538.000477.000126.080138.55020.10.7790.5560.22334551-18.572-24.76-3.960.190-9.8315.184.175.5
8.040 A15.00015.0002129.922112.762.2922.29120.720.30.7850.5540.23133333-18.683-24.510.190-9.83
8.040 B15.00015.00023686.010.7792112.222112.452.2932.29321.118.88.08480.000137.69020.80.7840.5580.22587946-18.105-24.67-1.980.190-9.8315.122.175.6
8.040 B15.00015.0002112.682.29321.321.00.7910.5610.23015443-17.977-24.500.190-9.83
8.040 C15.00015.00022949.010.7732113.392113.522.2972.29318.98.08485.000136.58017.30.6700.5680.10176954-18.377-24.6716.130.190-9.8315.025.5
8.040 C15.00015.0002113.652.28817.40.6740.5730.10056619-18.358-24.290.190-9.83
8.240 A15.00015.00013.58.2220511.010.7692131.762131.182121.482121.642.3842.3822.3712.37413.513.513.48.228.22346.000342.000185.940186.40019.20.8370.5380.29877028-19.853-25.64-6.520.190-9.8316.166.765.6
8.240 A15.00015.0002130.602121.792.3802.37613.718.10.7870.5610.22606532-18.994-25.530.190-9.83
8.240 B15.00015.00019572.010.7592122.972123.192.3872.38713.612.78.24325.000194.74016.80.7620.5530.20868522-18.824-25.47-0.560.190-9.8316.020.755.8
8.240 B15.00015.0002123.412.38713.416.80.7600.5650.19528128-18.855-25.430.190-9.83
8.240 C15.00015.00019784.010.7602121.222120.892.3852.38412.88.24326.000193.88020.40.9220.5470.37535868-17.954-26.02-6.120.190-9.8316.636.355.6
8.240 C15.00015.0002120.552.38220.50.9250.5520.37373016-17.954-26.040.190-9.83
8.890 A15.00015.0001.28.5620238.000.3752150.572150.782137.742138.532.9222.9242.9322.9301.21.21.08.568.5630.00026.000793.420823.82016.60.7290.4660.26254893-17.220-26.22-2.730.190-9.8316.802.925.8
8.890 A15.00015.0002150.982139.322.9262.9281.215.90.7450.4460.2992791-17.288-26.180.190-9.83
8.890 A15.00015.00021363.000.3822138.052138.262.9352.9281.21.08.5627.000819.66017.50.7270.4480.27909323-17.483-26.28-2.500.190-9.8316.822.705.9
8.890 A15.00015.0002138.462.9221.217.10.7130.4550.25797967-17.248-26.140.190-9.83
8.890 A15.00015.00021736.000.3842138.672138.342.9222.9211.18.5729.000801.90016.90.690-17.061-0.310.190-9.8316.980.505.5
8.890 A15.00015.0002138.012.92017.10.6980.4460.25207855-17.045-26.370.190-9.83
7.650 A30.00015.00030.27.8827954.011.1802085.092085.252037.052035.012.1842.1852.1562.15930.330.224.87.887.95772.000634.00086.70099.49027.31.2680.8150.45330082-16.174-25.12-0.430.190-9.8315.490.626.0
7.650 A30.00015.0002085.402032.972.1862.16229.927.61.2810.8160.46466177-16.054-24.770.190-9.83
7.650 B30.00015.00024329.011.1502040.792042.352.1682.16730.024.87.95634.000100.18023.71.2510.8790.37242048-16.236-25.223.050.190-9.8315.51-2.855.8
7.650 B30.00015.0002043.912.16630.424.01.2690.8520.41668882-16.135-24.690.190-9.83
7.650 C30.00015.00028272.011.1772038.902038.102.1692.16724.07.97613.000102.83026.21.1960.7960.40082743-16.572-24.97-2.320.190-9.8315.522.515.7
7.650 C30.00015.0002037.302.16627.11.242-17.5290.190-9.83
7.820 A30.00015.00025.47.9526342.011.1042093.632093.412053.992051.812.2252.2202.2032.20324.725.421.27.958.02650.000541.000102.610116.85029.71.2220.7710.45129778-16.111-25.21-0.540.190-9.8315.970.735.8
7.820 A30.00015.0002093.182049.622.21526.230.11.2360.7880.44815275-16.494-25.590.190-9.83
7.820 B30.00015.00028201.011.1152047.252048.172.1932.19726.121.48.02548.000115.21031.81.2230.7430.4803112-15.860-25.41-0.110.190-9.8316.090.305.9
7.820 B30.00015.0002049.092.20124.631.61.2140.7750.43937832-15.994-25.610.190-9.83
7.820 C30.00015.00025179.011.0882050.932050.342.2082.20620.68.03527.000119.46028.21.2120.7610.45064627-16.620-25.81-0.800.190-9.8316.300.996.1
7.820 C30.00015.0002049.752.20427.91.2000.7540.44516243-16.302-25.620.190-9.83
7.970 A30.00015.00020.08.0549516.011.1802102.892102.542053.522054.032.2692.2702.2432.24520.220.017.08.058.11512.000435.000128.670142.39037.81.0370.6220.41513459-15.575-25.68-0.460.190-9.8316.090.655.8
7.970 A30.00015.0002102.192054.532.2712.24620.037.51.0300.6260.40356214-15.612-25.360.190-9.83
7.970 B30.00015.00047183.011.1692051.452052.942.2462.24419.916.98.11433.000142.87038.31.1030.6520.45161505-15.660-25.92-1.070.190-9.8316.191.265.7
7.970 B30.00015.0002054.432.24320.138.31.1040.6460.45790693-15.346-25.310.190-9.83
7.970 C30.00015.00042388.011.1432059.192058.522.2452.24217.78.10454.000137.84034.81.1130.6700.44357936-15.631-25.55-1.000.190-9.8315.981.195.9
7.970 C30.00015.0002057.852.23935.21.1290.6770.45139738-15.704-25.270.190-9.83
8.040 A30.00015.00018.28.1035019.011.2022118.232118.902078.252078.172.3062.3072.2862.29218.118.215.48.108.16465.000394.000142.500158.68031.11.1600.6880.4722508-16.473-25.79-3.760.190-9.8316.233.976.1
8.040 A30.00015.0002119.562078.082.3072.29818.031.61.1820.6790.5034004-16.591-25.500.190-9.83
8.040 B30.00015.00037630.011.2122074.942074.722.2882.28618.215.68.15398.000156.85031.61.0970.6570.43948271-15.816-25.68-1.330.190-9.8316.161.535.8
8.040 B30.00015.0002074.502.28418.331.91.1100.6520.45764621-15.630-25.490.190-9.83
8.040 C30.00015.00032560.011.1922078.522078.652.2812.28216.28.14415.000151.97027.81.1560.820-16.150-25.16-2.900.190-9.8316.163.106.1
8.040 C30.00015.0002078.772.28428.31.1750.7030.47208939-17.067-26.000.190-9.83
8.240 A30.00015.00012.48.2543444.011.1112124.542124.472085.962086.882.3962.3952.3642.36412.312.411.68.258.27317.000298.000199.330203.09033.81.0540.6360.41833572-16.297-26.41-5.530.190-9.8316.965.766.2
8.240 A30.00015.0002124.392087.792.3932.36412.539.91.2500.6080.64145403-17.258-26.290.190-9.83
8.240 B30.00015.00044209.011.1112084.832085.202.3792.37312.511.18.29285.000210.66034.31.0520.5990.45373006-15.640-26.25-1.890.190-9.8316.762.086.0
8.240 B30.00015.0002085.562.36712.334.41.0560.5940.46247537-15.582-26.050.190-9.83
8.240 C30.00015.00041094.011.0952090.702090.202.3762.37411.48.28291.000207.58030.20.9950.5860.40882116-15.992-26.58-1.960.190-9.8317.022.155.7
8.240 C30.00015.0002089.692.37130.91.0190.5800.43973353-16.222-26.230.190-9.83
8.890 A30.00015.0000.98.5114745.000.6092169.572168.662159.202159.872.9682.9662.9492.9490.90.91.08.518.5323.00025.000869.880841.50019.01.0800.6640.41653964-18.260-24.67-7.380.190-9.8315.207.635.5
8.890 A30.00015.0002167.752160.532.9642.9490.916.41.1030.6840.41969617-17.828-24.650.190-9.83
8.890 A30.00015.00014217.000.6002160.002161.102.9652.9570.90.98.5224.000857.94018.91.1160.7200.39574162-17.940-25.66-4.690.190-9.8315.974.905.6
8.890 A30.00015.0002162.202.9480.919.41.1480.7260.42228851-17.888-25.130.190-9.83
8.890 A30.00015.00013492.000.5872158.462158.9518.61.2260.7940.43235097-17.809-24.91-6.700.190-9.8315.696.945.9
8.890 A30.00015.0002159.4319.41.2810.7950.48624858-18.946-25.350.190-9.83
7.650 A80.00015.00032.07.8621650.011.6822126.382126.952072.382071.352.2232.2222.1832.18331.832.027.57.867.91819.000704.00085.12093.31035.31.7731.1730.600125-17.502-26.740.350.190-9.8317.24-0.166.6
7.650 A80.00015.0002127.512070.312.2212.18332.035.01.7581.1660.59196152-17.517-26.480.190-9.83
7.650 B80.00015.00021805.011.6782060.312059.992.1782.18732.324.77.96632.000102.11039.11.9511.2660.68527849-17.361-26.67-0.150.190-9.8317.130.346.6
7.650 B80.00015.0002059.662.19632.039.01.9461.2730.67312021-17.283-26.350.190-9.83
7.650 C80.00015.00021850.011.6752072.442072.912.1852.18826.97.93688.00095.51036.71.8241.2070.6171287-17.605-26.810.340.190-9.8317.31-0.156.5
7.650 C80.00015.0002072.912.19236.81.8301.1880.64164772-17.146-26.560.190-9.83
7.820 A80.00015.00030.27.8932486.011.6062135.592133.232074.552073.882.2362.2372.1952.19831.030.225.57.897.95772.000652.00090.520100.53041.01.7181.0430.67482921-16.368-26.76-0.240.190-9.8317.160.436.3
7.820 A80.00015.0002130.872073.202.2392.20130.441.11.7231.0500.6733416-16.104-26.320.190-9.83
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