Citation:

bg-6-1877-2009

Name: Seawater carbonate chemistry, shell linear extension and calcification during calcein staining and 45Ca experiments with pteropod Limacina helicina, 2009
Published: 2009-08-27
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Arctic; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Limacina helicina; Mollusca; Pelagos; Polar; Single species; Zooplankton

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Abstract:

Thecosome pteropods (shelled pelagic molluscs) can play an important role in the food web of various ecosystems and play a key role in the cycling of carbon and carbonate. Since they harbor an aragonitic shell, they could be very sensitive to ocean acidification driven by the increase of anthropogenic CO2 emissions. The impact of changes in the carbonate chemistry was investigated on Limacina helicina, a key species of Arctic ecosystems. Pteropods were kept in culture under controlled pH conditions corresponding to pCO2 levels of 350 and 760 µatm. Calcification was estimated using a fluorochrome and the radioisotope 45Ca. It exhibits a 28 % decrease at the pH value expected for 2100 compared to the present pH value. This result supports the concern for the future of pteropods in a high-CO2 world, as well as of those species dependent upon them as a food resource. A decline of their populations would likely cause dramatic changes to the structure, function and services of polar ecosystems.

Keyword(s):

Animalia; Arctic; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Limacina helicina; Mollusca; Pelagos; Polar; Single species; Zooplankton

Project(s):

European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)

European Project on Ocean Acidification (EPOCA)

Ocean Acidification International Coordination Centre (OA-ICC)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Sixth Framework Programme (FP6), grant/award no. 511106 : European network of excellence for Ocean Ecosystems Analysis

Event(s):

Comeau_etal_09-45Ca * Method/Device: Experiment (EXP)

Comeau_etal_09-calcein * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event
2	Experimental treatment	Exp treat		Comeau, Steeve
3	Experiment day	Exp day	day	Comeau, Steeve
4	Carbonate system computation flag	CSC flag		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
5	Salinity	Sal		Comeau, Steeve
6	Temperature, water	Temp	°C	Comeau, Steeve
7	Alkalinity, total	AT	µmol/kg	Comeau, Steeve	Titration potentiometric
8	pH	pH		Comeau, Steeve	pH meter (Metrohm, 826 pH mobile)	Total scale
9	Carbon, inorganic, dissolved	DIC	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
10	Carbon dioxide	CO2	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
11	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
12	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
13	Bicarbonate ion	[HCO3]-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
14	Carbonate ion	[CO3]2-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
15	Aragonite saturation state	Omega Arg		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
16	Calcite saturation state	Omega Cal		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
17	Shell linear extension	Shell ext	mm	Comeau, Steeve	Measured
18	Calcification rate of calcium carbonate	Calc rate CaCO3	µmol/g/h	Comeau, Steeve	**45Ca incorporation

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

154 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Event	2 Exp treat	3 Exp day [day]	4 CSC flag	5 Sal	6 Temp [°C]	7 AT [µmol/kg]	8 pH	9 DIC [µmol/kg]	10 CO2 [µmol/kg]	11 pCO2water_SST_wet [µatm]	12 fCO2water_SST_wet [µatm]	13 [HCO3]- [µmol/kg]	14 [CO3]2- [µmol/kg]	15 Omega Arg	16 Omega Cal	17 Shell ext [mm]	18 Calc rate CaCO3 [µmol/g/h]
Comeau_etal_09-calcein	Control	1	8	34.8	4.2	2294	8.12	2120.0	17.30	323	322.00	1976.94	125.94	1.90	3.01
Comeau_etal_09-calcein	Control	2		34.8	4.1		8.08	2137.0		359				1.75
Comeau_etal_09-calcein	Control	3	8	34.8	4.3	2296	8.06	2146.0	20.17	378	376.86	2013.58	112.17	1.69	2.68
Comeau_etal_09-calcein	Control	4		34.8	4.0		8.09	2136.0		350				1.78
Comeau_etal_09-calcein	Control	5	8	34.8	4.2	2292	8.11	2122.0	17.74	331	330.25	1981.39	123.35	1.86	2.95	0.600
Comeau_etal_09-calcein	Low pH condition	1	8	34.8	4.2	2297	7.77	2246.0	41.99	784	781.52	2143.24	60.99	0.91	1.46
Comeau_etal_09-calcein	Low pH condition	2		34.8	4.1		7.82	2231.0		693				1.02
Comeau_etal_09-calcein	Low pH condition	3	8	34.8	4.3	2292	7.83	2222.0	36.02	675	672.85	2116.90	69.44	1.05	1.66
Comeau_etal_09-calcein	Low pH condition	4		34.8	4.0		7.77	2242.0		782				0.91
Comeau_etal_09-calcein	Low pH condition	5	8	34.8	4.2	2297	7.79	2238.0	39.97	746	744.00	2136.51	63.66	0.96	1.52	0.275
Comeau_etal_09-45Ca	Control		8	34.8	5.0	2295	7.78	2237.0	39.93	765	765.08	2133.43	64.16	1.00	1.53		0.26
Comeau_etal_09-45Ca	Low pH condition		8	34.8	5.0	2298	8.09	2131.0	18.25	350	349.65	1990.70	122.23	1.90	2.92		0.36