Roberts Dai d.roberts@qub.ac.uk http://www.qub.ac.uk/schools/SchoolofBiologicalSciences/Staff/DrDRoberts/ Moore H M Berges J 0000-0002-3124-4783 Patching John W john.patching@NUIGalway.ie Carton Micheal W Micheal.Carton@NUIGalway.ie Eardly Donal F DONAL.EARDLY@NUIGalway.ie 0000-0002-4123-5127 2001 Gut content of holothurians from the NE Atlantic dataset publication series https://doi.org/10.1594/PANGAEA.724280 Roberts Dai d.roberts@qub.ac.uk http://www.qub.ac.uk/schools/SchoolofBiologicalSciences/Staff/DrDRoberts/ Moore H M Berges J 0000-0002-3124-4783 Patching John W john.patching@NUIGalway.ie Carton Micheal W Micheal.Carton@NUIGalway.ie Eardly Donal F DONAL.EARDLY@NUIGalway.ie 0000-0002-4123-5127 2001 Sediment distribution, hydrolytic enzyme profiles and bacterial activities in the guts of Oneirophanta mutabilis, Psychropotes longicauda and Pseudostichopus villosus: what do they tell us about digestive strategies of abyssal holothurians? Progress in Oceanography 50(1-4) https://doi.org/10.1016/S0079-6611(01)00065-9 443-458 This paper describes inter-specific differences in the distribution of sediment in the gut compartments and in the enzyme and bacterial profiles along the gut of abyssal holothurian species - Oneirophanta mutabilis, Psychropotes longicauda and Pseudostichopus villosus sampled from a eutrophic site in the NE Atlantic at different times of the year. Proportions of sediments, relative to total gut contents, in the pharynx, oesophagus, anterior and posterior intestine differed significantly in all the inter-species comparisons, but not between inter-seasonal comparisons. Significant differences were also found between the relative proportions of sediments in both the rectum and cloaca of Psychropotes longicauda and Oneirophanta mutabilis. Nineteen enzymes were identified in either gut-tissue or gut-content samples of the holothurians studied. Concentrations of the enzymes in gut tissues and their contents were highly correlated. Greater concentrations of the enzymes were found in the gut tissues suggesting that they are the main source of the enzymes. The suites of enzymes recorded were broadly similar in each of the species sampled collected regardless of the time of the year, and they were similar to those described previously for shallow-water holothurians. Significant inter-specific differences in the gut tissue concentrations of some of the glycosidases suggest dietary differences. For example, Psychropotes longicauda and Pseudostichopus villosus contain higher levels of chitobiase than Oneirophanta mutabilis. There were no seasonal changes in bacterial activity profiles along the guts of O. mutabilis and Pseudostichopus villosus. In both these species bacterial activity and abundance declined between the pharynx/oesophagus and anterior intestine, but then increased along the gut and became greatest in the rectum/cloaca. Although the data sets were more limited for Psychropotes longicauda, bacterial activity increased from the anterior to the posterior intestine but then declined slightly to the rectum/cloaca. These changes in bacterial activity and densities probably reflect changes in the microbial environment along the guts of abyssal holothurians. Such changes suggest that there is potential for microbial breakdown of a broader range of substrates than could be otherwise be achieved by the holothurian itself. However, the present study found no evidence for sedimentary (microbial) sources of hydrolytic enzymes. -16.715999599999975 -16.443000789999985 48.82699966 48.92399979 -16.521200369999917 48.865099714999985 1996-09-04T15:45:00 1997-04-07T11:54:00 BENGAL Benthic Biology and Geochemistry of a North-eastern Atlantic Abyssal Locality https://en.wikipedia.org/wiki/BENGAL_(project) National Oceanography Centre, Southampton NOCS http://www.soc.soton.ac.uk/ https://ror.org/00874hx02 Fourth Framework Programme FP4 https://doi.org/10.13039/100011105 MAS3950018 Benthic Biology and Geochemistry of a North-eastern Atlantic Abyssal Locality https://cordis.europa.eu/project/id/MAS3950018 12930-026 48.88199997 -16.7159996 1996-09-04T15:45:00 48.84999847 -16.54999924 1996-09-04T18:35:00 D222/2 D222/2 1996-08-29T00:00:00 1996-08-29 1996-09-24T00:00:00 1996-09-24 Discovery (1962) https://en.wikipedia.org/wiki/RRS_Discovery_(1962) Rice, Anthony Leonard Rice, Anthony Leonard also cruise for BENGAL Discovery (1962) https://en.wikipedia.org/wiki/RRS_Discovery_(1962) Semi-balloon trawl OTSB14 Good catch 12930-037 48.85400009 -16.49500084 -4837.0 1996-09-06T17:48:00 48.85400009 -16.49500084 1996-09-06T17:48:00 D222/2 D222/2 1996-08-29T00:00:00 1996-08-29 1996-09-24T00:00:00 1996-09-24 Discovery (1962) https://en.wikipedia.org/wiki/RRS_Discovery_(1962) Rice, Anthony Leonard Rice, Anthony Leonard also cruise for BENGAL Discovery (1962) https://en.wikipedia.org/wiki/RRS_Discovery_(1962) Semi-balloon trawl OTSB14 12 good cores 13078-011 48.83399963 -16.50200081 -4846.0 1997-04-01T00:52:00 48.83599854 -16.50600052 1997-04-01T04:45:00 D226 D226 1997-03-12T00:00:00 1997-03-12 1997-04-10T00:00:00 1997-04-10 Discovery (1962) https://en.wikipedia.org/wiki/RRS_Discovery_(1962) Rice, Anthony Leonard Rice, Anthony Leonard also cruise for BENGAL Discovery (1962) https://en.wikipedia.org/wiki/RRS_Discovery_(1962) Chalut à perche (6 m beam trawl) CP Bottles at 4826-4775m. Wire tests 13078-029 48.92100143 -16.44300079 -4846.0 1997-04-04T04:09:00 48.92399979 -16.44400024 1997-04-04T04:29:00 D226 D226 1997-03-12T00:00:00 1997-03-12 1997-04-10T00:00:00 1997-04-10 Discovery (1962) https://en.wikipedia.org/wiki/RRS_Discovery_(1962) Rice, Anthony Leonard Rice, Anthony Leonard also cruise for BENGAL Discovery (1962) https://en.wikipedia.org/wiki/RRS_Discovery_(1962) Semi-balloon trawl OTSB14 Time series, bottle at 4m 13078-037 48.86899948 -16.5890007 -4843.0 1997-04-07T10:02:00 48.82699966 -16.47200012 1997-04-07T11:54:00 D226 D226 1997-03-12T00:00:00 1997-03-12 1997-04-10T00:00:00 1997-04-10 Discovery (1962) https://en.wikipedia.org/wiki/RRS_Discovery_(1962) Rice, Anthony Leonard Rice, Anthony Leonard also cruise for BENGAL Discovery (1962) https://en.wikipedia.org/wiki/RRS_Discovery_(1962) Semi-balloon trawl OTSB14 Clean catch, many fish. 10 CC-BY-3.0 Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/ Species | Enzyme group | Enzyme gr | Enzyme | Enzyme code | Enzyme cd | Pharynx/oesophagus, scale of 0 to 5 | Relative enzyme activity in gut content | REA gut con | Anterior intestine, scale of 0 to 5 | Posterior intestine, scale of 0 to 5 | Rectum/cloaca, scale of 0 to 5 | Pharynx/oesophagus | Relative enzyme activity in gut content, standard deviation | REA gut con std dev | Anterior intestine | Posterior intestine | Rectum/cloaca | Relative enzyme activity in gut tissue | REA gut tissue | Relative enzyme activity in gut tissue, standard deviation | REA gut tissue std dev supplement citable param7053 param83672 param83673 param83674 param83675 param83676 param83678 param83679 pi20323 author20322 author20323 author20329 author20330 author34922 author34923 award88 basis42 campaign3382911 campaign3382912 campaign3532 campaign3533 event2040547 event2040558 event2040728 event2040746 event2040754 funder20652 geocode1599 geocode1600 geocode1601 geocode8128 inst64 journal13363 license101 method10876 method11604 project85 ref30291