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Burkhardt, Steffen; Riebesell, Ulf; Zondervan, Ingrid (1999): Seawater carbonate chemistry, stable carbon isotope fractionation and growth rate during experiments with marine phytoplankton community, 1999. PANGAEA, https://doi.org/10.1594/PANGAEA.718103, Supplement to: Burkhardt, S et al. (1999): Stable carbon isotope fractionation by marine phytoplankton in response to daylength, growth rate, and CO2 availability. Marine Ecology Progress Series, 194, 31-41, https://doi.org/10.3354/meps184031

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Abstract:
Stable carbon isotope fractionation (%) of 7 marine phytoplankton species grown in different irradiance cycles was measured under nutrient-replete conditions at a high light intensity in batch cultures. Compared to experiments under continuous light, all species exhibited a significantly higher instantaneous growth rate (pi), defined as the rate of carbon fixation during the photo period, when cultivated at 12:12 h. 16:8 h, or 186 h light:dark (L/D) cycles. Isotopic fractionation by the diatoms Skeletonema costatum, Asterionella glacialis, Thalassiosira punctigera, and Coscinodiscus wailesii (Group I) was 4 to 6% lower in a 16:8 h L/D cycle than under continuous light, which we attribute to differences in pi. In contrast, E, in Phaeodactylum tn'cornutum, Thalassiosira weissflogii, and in the dinoflagellate Scrippsiella trochoidea (Group 11) was largely insensitive to day length-related differences in instantaneous growth rate. Since other studies have reported growth-rate dependent fractionation under N-limited conditions in P. tricornutum, pi-related effects on fractionation apparently depend on the factor controlling growth rate. We suggest that a general relationship between E, and pi/[C02,,,] may not exist. For 1 species of each group we tested the effect of variable CO2 concentration, [COz,,,], on isotopic fractionation. A decrease in [CO2,,,] from ca 26 to 3 pm01 kg-' caused a decrease in E, by less than 3%0 This indicates that variation in h in response to changes in day length has a similar or even greater effect on isotopic fractionation than [COz,,,] m some of the species tested. In both groups E, tended to be higher in smaller species at comparable growth rates. In 24 and 48 h time series the algal cells became progressively enriched in 13C during the day and the first hours of the dark period, followed by l3C depletion in the 2 h before beginning of the following Light period. The daily amplitude of the algal isotopic composition (613C), however, was <1.5%0, which demonstrates that diurnal variation in Fl3C is relatively small.
Keyword(s):
Asterionella glacialis; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria ( 20 L); Chromista; Coscinodiscus wailesii; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Myzozoa; Not applicable; Ochrophyta; Pelagos; Phaedactylum tricornutum; Phytoplankton; Scrippsiella trochoidea; Single species; Skeletonema costatum; Thalassiosira punctigera; Thalassiosira weissflogii
Event(s):
Burkhardt_etal_99 * Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1SpeciesSpeciesBurkhardt, Steffen
2Experimental treatmentExp treatBurkhardt, Steffen
3Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
4SalinitySalBurkhardt, Steffen
5Temperature, waterTemp°CBurkhardt, Steffen
6Radiation, photosynthetically activePARµmol/m2/sBurkhardt, Steffen
7Light:Dark cycleL:Dhh:hhBurkhardt, SteffenMeasured
8pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
9Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
10Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
11Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
12Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
13Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
14Carbon, inorganic, dissolvedDICµmol/kgBurkhardt, SteffenCoulometric titration
15Alkalinity, totalATµmol/kgBurkhardt, SteffenAlkalinity, Gran titration (Gran, 1950)
16Aragonite saturation stateOmega ArgBurkhardt, SteffenCalculated
17Calcite saturation stateOmega CalBurkhardt, SteffenCalculated
18SilicateSILCATµmol/kgBurkhardt, Steffen
19PhosphatePHSPHTµmol/kgBurkhardt, Steffen
20NitrateNITRATµmol/kgBurkhardt, Steffen
21δ13C, carbon dioxide, aquaticδ13C CO2 aqBurkhardt, SteffenMass spectrometer Finnigan Delta-S
22δ13C, particulate organic carbonδ13C POC‰ PDBBurkhardt, SteffenMass spectrometer ANCA-SL 20-20 Europa Scientific
23Isotopic fractionation, during photosynthisepsilon pBurkhardt, SteffenCalculated after Freeman & Hayes (1992)
24Growth rateµ#/dayBurkhardt, SteffenCalculated
Size:
480 data points

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