Christaki, Urania (2008): Microbe abundance and biomass at station SEPT-1999-K1 in the Northeastern Aegean Sea [dataset]. Université du Littoral Côte d'Opale, PANGAEA, https://doi.org/10.1594/PANGAEA.688623
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Project(s):
Funding:
Fourth Framework Programme (FP4), grant/award no. MAS3970148: Key coastal processes in the mesotropic Skagerrak and the oligotrophic Northern Aegean: a comparative study
Sixth Framework Programme (FP6), grant/award no. 36949: Southern European Seas: Assessing and Modelling Ecosystem Changes
Coverage:
Latitude: 39.429167 * Longitude: 25.733333
Date/Time Start: 1999-09-22T12:00:00 * Date/Time End: 1999-09-22T12:00:00
Minimum DEPTH, water: 5 m * Maximum DEPTH, water: 100 m
Event(s):
SEPT-1999-K1 * Latitude: 39.429167 * Longitude: 25.733333 * Date/Time: 1999-09-22T12:00:00 * Campaign: SEPT-1999 * Basis: Aegaeo * Method/Device: Bottle, Niskin 8-L (NIS_8L)
Comment:
Borax-buffered formalin (final concentration 2% formaldehyde). Slides for nanoflagellates counting were stored at -20°C. Subsamples (10-30 ml) were concentrated on 25mm black polycarbonate filters of porosity 0.6µm for nanoflagellates, stained with DAPI for 10 min (Porter & Feig 1980) and filtered. Heterotrophic (HF) and photototrophic (PF) nanoflagellates were distinguished using UV and blue excitation and enumerated. Nanoflagellates were classified in size categories and the biovolume was calculated.
Abundance data were converted into C biomass using 220fgC um-3 (Borsheim & Bratbak 1987) for nanoflagellates.
Bacterial production was estimated by the 3H-leucine method (Kirchman et al. 1986, Kirchman 1993). At each depth, duplicate samples and a control were incubated with 1 nM L-[4,5 3H]-leucine (specific activity 128 Ci mmol-1) and 19 nM non radioactive leucine. Samples were incubated in the dark, at in situ temperature.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | DEPTH, water | Depth water | m | Geocode | ||
| 2 | Nanoflagellates, phototrophic, biomass as carbon | PNF C | µg/l | Christaki, Urania | Borsheim & Bratbak, 1987; cell carbon conversion | |
| 3 | Nanoflagellates, heterotrophic, biomass as carbon | HNF C | µg/l | Christaki, Urania | Borsheim & Bratbak, 1987; cell carbon conversion | |
| 4 | Bacteria, biomass as carbon | Bact C | µg/l | Christaki, Urania | Lee & Fuhrman, 1987, bacteria biomass | |
| 5 | Nanoflagellates, phototrophic | PNF | #/ml | Christaki, Urania | Porter & Feig, 1980 (Limn. Oceanogr. 25, 943-948) | |
| 6 | Nanoflagellates, heterotrophic | HNF | #/ml | Christaki, Urania | Porter & Feig, 1980 (Limn. Oceanogr. 25, 943-948) | |
| 7 | Bacteria | Bact | #/ml | Christaki, Urania | Porter & Feig, 1980 (Limn. Oceanogr. 25, 943-948) | |
| 8 | Bacteria, production as carbon | BCP | ng/l/h | Christaki, Urania | Kirchman et al., 1986 |
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
35 data points