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Tabrizicola aquatica gen. nov. sp. nov., a novel alphaproteobacterium isolated from Qurugöl Lake nearby Tabriz city, Iran

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Abstract

A novel Gram-negative, aerobic, non-motile and rod-shaped bacterium was isolated from Qurugöl Lake near Tabriz city. The bacterium grew chemoorganolheterotrophically and chemolithoautotrophically. However, photo-organoheterotrophic, photo-lithoautotrophic and fermentative growth could not be demonstrated. The presence of photosynthesis genes pufL and pufM was not shown and photosynthesis pigments were not formed. Strain RCRI19T grew without NaCl and tolerated up to 3 % NaCl. Growth occurred at pH 6–9 (optimum, pH 7) and 15–55 °C (optimum 40–45 °C). Vitamins were not required for growth. The major fatty acids are C18:1 ω7C, 11-methyl C18:1 ω7C, C18:0 3-OH. The predominant respiratory quinone is ubiquinone Q-10. The G+C content of genomic DNA is 65.9 mol%. Analysis of 16S rRNA sequences showed that strain RCRI19T has the highest similarities with uncultured environmental sequences followed by members of the genera Rhodobacter (≤95.75 %), Haematobacter (≤95.53 %), Gemmobacter (≤95.17 %) and Falsirhodobacter (94.60 %) in the family Rhodobacteraceae. DNA–DNA relatedness between strain RCRI19T and the closest phylogenetically related strain, Rhodobacter blasticus LMG 4305T, was 20 %. Based on its phenotypic and chemotaxonomic characteristics and considering that it does not form photosynthetic pigments and is unable to grow phototrophically, it is concluded that strain RCRI19T cannot be included into the genus Rhodobacter and any of the other related genera. Therefore, we propose to place the new bacterium into a new genus and species for which the name Tabrizicola aquatica gen. nov. and sp. nov. is proposed. The type strain is RCRI19T (=BCCM/LMG 25773T = JCM 17277T = KCTC 23724T).

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Acknowledgments

This study has been conducted in collaboration between Pharmaceutical Biotechnology Department, Faculty of Pharmacy, Tabriz University of Medical Sciences and Rice and Citrus Research Institute, Sari Agricultural and Natural Resources University. The authors greatly thank Dr Cathrin Spröer (Deutsche Sammlung von Mikroorganismen und Zellkulturen; DSMZ) for her advice in 16S rRNA sequence analysis.

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Authors and Affiliations

  1. Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

    Vahideh Tarhriz & Mohammad Saeid Hejazi

  2. Tabarestan Research Center for Genetic and Agriculture Biotechnology, Sari, Iran

    Vahideh Tarhriz & Ghorbanali Nematzadeh

  3. Marine Mikrobiologie, Helmholtz Centre for Ocean Research GEOMAR, Düsternbrooker Weg 20, Kiel, Germany

    Vera Thiel & Johannes F. Imhoff

  4. Agricultural Biotechnology Research Institute (ABRII) for Northwest and West of Iran, Tabriz, Iran

    Mohammad Amin Hejazi

  5. School of Advanced Biomedical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Saeid Hejazi

Authors
  1. Vahideh Tarhriz
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  2. Vera Thiel
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  3. Ghorbanali Nematzadeh
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  4. Mohammad Amin Hejazi
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  5. Johannes F. Imhoff
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  6. Mohammad Saeid Hejazi
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Corresponding authors

Correspondence to Johannes F. Imhoff or Mohammad Saeid Hejazi.

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Tarhriz, V., Thiel, V., Nematzadeh, G. et al. Tabrizicola aquatica gen. nov. sp. nov., a novel alphaproteobacterium isolated from Qurugöl Lake nearby Tabriz city, Iran. Antonie van Leeuwenhoek 104, 1205–1215 (2013). https://doi.org/10.1007/s10482-013-0042-y

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