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Glaciibacter flavus sp. nov., isolated from a lichen sample

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Abstract

A novel Actinobacterium strain YIM 131861 T, was isolated from lichen collected from the South Bank Forest of the Baltic Sea, Germany. It was Gram-stain-positive, strictly aerobic, catalase positive and oxidase negative, yellow pigmented. Cells were motile with a polar flagellum, irregular rod shaped and did not display spore formation. The strain grew at 15 − 30 °C (optimum 25 °C), at pH 6.0 − 10.0 (optimum pH 7.0) and in the presence of 0 − 1.5% (w/v) NaCl (optimum 1%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 131861 T belonged to the genus Glaciibacter, and exhibited a high sequence similarity (96.4%) with Glaciibacter superstes NBRC 104264 T. The genomic DNA G + C content of strain YIM 131861 T was 68.2 mol%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain YIM 131861 T and Glaciibacter superstes NBRC 104264 T were 73.2 and 19.9% based on the draft genome sequence. The cell-wall peptidoglycan type was B2γ and contained the 2, 4-diaminobutyric acid as the diagnostic amino acid. Whole cell sugars were galactose, rhamnose, ribose and glucose. It contained MK-12 and MK-13 as the predominant menaquinones. The major cellular fatty acids (> 10%) were identified as anteiso-C15:0, iso-C16:0 and anteiso-C17:0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain YIM 131861 T should belong to the genus Glaciibacter and represents a novel species of the genus Glaciibacter, for which the name Glaciibacter flavus sp. nov. is proposed. The type strain is YIM 131861 T (= CGMCC 1.16588 T = NBRC 113572 T).

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Abbreviations

R2A:

Reasoner’s 2A

TSA:

Tryptic soy agar

TSB:

Tryptic soy broth

ANI:

Average Nucleotide Identity

dDDH:

Digital DNA–DNA hybridization

DPG:

Diphosphatidylglycerol

PG:

Phosphatidylglycerol

GL:

Glycolipid

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Acknowledgements

This research was funded by National Natural Science Foundation of China (31460005 and 32060001) and Major research project of Guangxi for science and technology (AA18242026). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YIM 131861T is MK608324 and the genome sequence is SSSN00000000.

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Author notes

  1. De-Feng An and Long-Qian Jiang equally contributed to this work.

Authors and Affiliations

  1. Yunnan Institute of Microbiology, Key Laboratory for Conservation and Utilization of Bio-Resource, School of Life Sciences, Yunnan University, Kunming, People’s Republic of China

    De-Feng An, Long-Qian Jiang, Kun Zhang, Gui-Ding Li, Lei Lang, Cheng-Lin Jiang & Yi Jiang

  2. Institute of Microbial Pharmaceuticals, Northeastern University, Shenyang, 110819, People’s Republic of China

    Gui-Ding Li

  3. Key Lab for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People’s Republic of China

    Xin-Yu Wang & Li-Song Wang

  4. School of Marine Science and Biotechnology, Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi University for Nationalities, Nanning, 530008, People’s Republic of China

    Ming-Guo Jiang

  5. GEOMAR Helmholtz Centre for Ocean Research Kiel, Wischhofstr. 1-3, 24148, Kiel, Germany

    Johannes F. Imhoff

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  1. De-Feng An
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Contributions

DFA and LQJ performed the experiments and wrote the manuscript; JFI collected the lichen samples; KZ, GDL, XYW and LL analysed the data; MGJ identified the lichen samples; YJ guided the experiments and revised the manuscript; CLJ and LSW designed the study.

Corresponding author

Correspondence to Yi Jiang.

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Communicated by Erko Stackebrandt.

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An, DF., Jiang, LQ., Zhang, K. et al. Glaciibacter flavus sp. nov., isolated from a lichen sample. Arch Microbiol 203, 2439–2444 (2021). https://doi.org/10.1007/s00203-021-02247-0

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  • DOI: https://doi.org/10.1007/s00203-021-02247-0

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