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QTL analysis of root-lesion nematode resistance in barley: 1. Pratylenchus neglectus

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Abstract

The root-lesion nematode Pratylenchus neglectus can cause severe losses in barley cultivation. Multiplication rates had been found to vary greatly between different barley accessions. Two winter barley cultivars, Igri and Franka, had been found to differ in their ability to resist this parasite. An existing Igri × Franka doubled haploid population was chosen to genetically map resistance genes after artificial inoculation with P. neglectus in the greenhouse and climate chamber. A continuous phenotypic variation was found indicating a quantitative inheritance of P. neglectus resistance. An existing map was enriched by 527 newly developed Diversity Array Technology markers (DArTs). The new genetic linkage map was comprised of 857 molecular markers that cover 1,157 cM on seven linkage groups. Using phenotypic data collected from four different experiments in 3 years, five quantitative trait loci were mapped by composite interval mapping on four (3H, 5H, 6H and 7H) linkage groups. A quantitative trait locus with a large phenotypic effect of 16% and likelihood of odds (LOD) score of 6.35 was mapped on linkage group 3H. The remaining four QTLs were classified as minor or moderate with LOD scores ranging from 2.71 to 3.55 and R 2 values ranging from 8 to 10%. The DNA markers linked to the resistance QTLs should be quite useful for marker-assisted selection in barley breeding because phenotypic selection is limited due to time constraints and labor costs.

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Acknowledgments

The authors gratefully acknowledge Bärbel Wohnsen, Cay Kruse and Ines Schütt for their excellent technical assistance during the greenhouse resistance tests. This work was financially supported by the BLE grant No. 28-1-41.030-06 and the Nordsaat GmbH (Böhnshausen, Germany).

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Authors and Affiliations

  1. Plant Breeding Institute, Christian-Albrechts-University of Kiel, Olshausenstrasse 40, 24118, Kiel, Germany

    Shiveta Sharma, Shailendra Sharma, Friedrich J. Kopisch-Obuch, Tobias Keil & Christian Jung

  2. Nordsaat Saatzucht GmbH, Hofweg 8, 23899, Segrahn, Germany

    Eberhard Laubach

  3. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466, Gatersleben, Germany

    Nils Stein & Andreas Graner

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  1. Shiveta Sharma
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  2. Shailendra Sharma
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  3. Friedrich J. Kopisch-Obuch
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  4. Tobias Keil
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  5. Eberhard Laubach
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  6. Nils Stein
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  7. Andreas Graner
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  8. Christian Jung
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Corresponding author

Correspondence to Christian Jung.

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Communicated by P. Hayes.

Electronic supplementary material

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122_2011_1533_MOESM1_ESM.pptx

Supplementary Figure 1a-d: Chromosomal location of QTL for P. neglectus resistance in the barley DH population Igri X Franka. DH lines were tested in four different environments. (PPTX 66 kb)

Supplementary material 2 (PPTX 73 kb)

Supplementary material 3 (PPTX 67 kb)

Supplementary material 4 (PPTX 73 kb)

122_2011_1533_MOESM5_ESM.doc

Supplementary Table 1: Multiplication rates (Pf/Pi) of P. neglectus in 126 Igri X Franka DH and two parents observed across all environments (DOC 121 kb)

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Sharma, S., Sharma, S., Kopisch-Obuch, F.J. et al. QTL analysis of root-lesion nematode resistance in barley: 1. Pratylenchus neglectus . Theor Appl Genet 122, 1321–1330 (2011). https://doi.org/10.1007/s00122-011-1533-8

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  • DOI: https://doi.org/10.1007/s00122-011-1533-8

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