Abstract
Colonial non-zooxanthellate corals from deep-water coral reefs, Lophelia pertusa and Madrepora oculata, produce large amounts of extracellular mucus (EMS). This mucus has various functions, e.g., an antifouling capability protecting the coral skeleton from attacks of endolithic and boring organisms. Both corals show thick epithecal and exothecal skeletal parts with a clear lamellar growth pattern. The formation of the epitheca is unclear. It is supposed that the EMS play a central role during the calcification process of the epithecal skeletal parts. Staining with the fluorochrome tetracycline has shown an enrichment of Ca2+ ions in the mucus. In order to investigate this hypothesis, the protein content of the mucus and the intracrystalline organic matter from newly formed epithecal aragonite of Madrepora oculata was determined via sodium dodecyl sulfate (SDS) gel electrophoresis. Identical band patterns within both substances could be detected, one around 45 kDa molecular weight and a cluster around 30–35 kDa molecular weight. The occurrence of identical protein patterns within the mucus and in the newly formed aragonite confirms the idea that the mucus plays an important role during the organomineralization of the coral epitheca.
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References
Addadi L, Weiner S (1985) Interactions between acidic proteins and crystals: stereochemical requirements in biomineralisation. Proc Natl Acad Sci USA 82: 4110–4114
Addadi L, Weiner S (1992) Kontroll-und Designprinzipien bei der Biomineralisation. Angew Chem 104: 159–176
Allemand D, Tambutté È, Girard J-P, Jaubert J (2001) Organic matrix synthesis in the scleractinian coral Stylophora pistillata: role in biomineralization and potential target of the organotin tributyltin. J Exp Biol: 201: 2001–2009
Arp G, Reimer A, Reitner J (2003) Microbialite formation in seawater of increased alkalinity, Satonda Crater Lake, Indonesia. J Sediment Res 73: 105–127
Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254
Bryan W, Hill D (1941) Spherulitic crystallization as a mechanism of skeletal growth in the Hexacorals. Proc R Soc Queensland 52: 78–91
Constantz BR (1986) Coral skeleton construction: a physiochemically dominated process. Palaios 1: 152–157
Constantz BR, Meike A (1989) Calcite centres of calcification in Mussa angulosa (Scleractinia). In: Crick RE (ed) Origin, Evolution and modern Aspects of Biomineralization in Plants and Animals. Plenum Press, New York, pp 201–207
Constantz BR, Weiner S (1988) Acidic macromolecules associated with the mineral phase of scleractinian coral skeletons. J Exp Zool 248: 253–258
Cuif J-P, Dauphin Y (1998) Microstructural and physico-chemical characterization of “centers of calcification” in septa of some Recent scleractinian corals. Paläont Z 72: 257–270
Cuif J-P, Dauphin Y, Denis A, Gautret P (1996) The organomineral structure of coral skeletons: a potential source of new criteria for scleractinian taxonomy. Bull Inst Océanogr Monaco Spec Issue 14: 359–367
Cuif J-P, Dauphin Y, Gautret P (1999) Compositional diversity of soluble mineralizing matrices in some recent coral skeletons compared to fine-scale growth structures of fibres: discussion of consequences for biomineralization and diagenesis. Int J Earth Sci 88: 582–592
Cuif J-P, Gautret P (1995) Gluides et proteins de la matrice soluble des biocristaux de scleractiniaires acroporides. CR Acad Sci Paris 320 Ser IIa: 273–278
Dauphin Y, Cuif J-P (1997) Isoelectric properties of the soluble matrices in relation to the chemical composition of some scleractinian skeletons. Electrophoresis 18: 1180–1183
Defarge C, Trichet J (1995) From biominerals to “organominerals”: the example of the modern lacustrine calcareous stromatolites from Polynesian atolls. Bull Inst Océanogr Monaco Spec Issue 14: 265–271
Freiwald A, Wilson JB (1998) Taphonomy of modern deep, cold-temperate water coral reefs. Hist Biol 13: 37–52
Freiwald A, Henrich R, Pätzold J (1997) Anatomy of a deep-water coral reef mound from Stjernsund. SEPM Spec Publ 56: 141–161
Freiwald A, Hühnerbach V, Lindberg B, Wilson JB, Campbell J (2002) The Sula Reef Complex, Norwegian Shelf. Facies 47: 179–200
Gautret P, Cuif, J-P, Freiwald A (1997) Composition of soluble mineralizing matrices in zooxanthellate and non-zooxanthellate scleractinian corals: biochemical assessment of photosynthetic metabolism through the study of a skeletal feature. Facies 36: 189–194
Gladfelter EH (1984) Skeletal development in Acropora cervicornis. A comparison of monthly rates of linear extension and calcium carbonate accretion measured over a year. Coral Reefs 3: 51–57
Goreau T (1956) Histochemistry of mucopolysaccharide-like substances and alkaline phosphatase in Madreporaria. Nature 177: 1029–1030
Gunthorpe ME, Sikes CS, Wheeler AP (1990) Promotion and inhibition of calcium carbonate crystallization in vitro by matrix protein from blue crab exoskeleton. Biol Bull 179: 191–200
Johnston I (1980) The ultrastructure of skeletogenesis in hermatypic corals. Int Rev Cyt 67: 171–214
Lange R, Bergbauer M, Szewzyk U, Reitner J (2001) Soluble proteins control growth of skeleton crystals in three coralline demosponges. Facies 45: 195–202
Milliman JD (1974) Marine carbonates. In: Milliman JD, Müller G, Förstner U (eds) Recent sedimentary Carbonates. Springer, Berlin
Mitterer RM (1978) Amino acid composition and metal binding capability of the skeletal protein of corals. Bull Mar Sci 28: 173–180
Reitner J (1993) Modern cryptic microbialite/metazoan facies from Lizard Island (Great Barrier Reef, Australia). Formation and concepts. Facies 29: 3–39
Reitner J, Hoffmann F (2003) Schwämme in Kaltwasser-Korallenriffen. Kleine Senckenberg-Reihe 45: 75–87
Reitner J, Gautret P, Marin F, Neuweiler F (1995) Automicrites in a modern marine microbialite. Formation model via organic matrices (Lizard Island, Great Barrier Reef, Australia). Bull Inst Océanogr Monaco Spec Issue 14: 237–263
Reitner J, Wörheide G, Lange R, Schumann-Kindel G (2001) Coralline demosponges — a geobiological portait. Bull Tohoku Univ Mus 1: 219–235
Stolarski J (2003) Three-dimensional micro-and nanostructural characteristics of the scleractinian coral skeleton: a biocalcification proxy. Acta Palaeontol Pol 48: 497–530
Trichet J, Defarge C (1995) Non-biologically supported organomineralisation. Bull Inst Océanogr Monaco Spec Issue 14: 203–236
Weiner S, Traub W, Lowenstam HA (1983) Organic matrix in calcified exoskeletons. In: Westbroek P, de Jong EW (eds) Biomineralization and Biological Metal Accumulation. Reidel, Amsterdam, pp 205–224
Wheeler AP, George JW, Evans CA (1981) Control of calcium carbonate nucleation and crystal growth by soluble matrix of oyster shell. Science 212: 1397–1398
Wilbur KM, Simkiss K (1979) Carbonate turnover and depostion by Metazoa. In: Trudinger PA, Swaine DJ (eds) Studies in Environmental Sciences. Biochemical Cycling of Mineral-forming Elements. Elsevier, Amsterdam, pp 69–106
Wörheide G, Gautret P, Reitner J, Böhm F, Joachimski MM, Thiel V, Michaelis W, Massault M (1997) Basal skeletal formation, role and preservation of intracrystalline organic matrices, and isotopic record in the coralline sponge Astrosclera willeyana Lister, 1900. Bol R Soc Esp Hist Nat (Sec Geol) 91: 355–374
Young SD (1971) Organic material from scleractinian coral skeleton. 1. Variation in composition between several species. Cop Biochem Physiol 40B: 113–120
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Reitner, J. (2005). Calcifying extracellular mucus substances (EMS) of Madrepora oculata — a first geobiological approach. In: Freiwald, A., Roberts, J.M. (eds) Cold-Water Corals and Ecosystems. Erlangen Earth Conference Series. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-27673-4_38
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DOI: https://doi.org/10.1007/3-540-27673-4_38
Publisher Name: Springer, Berlin, Heidelberg
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