/* DATA DESCRIPTION: Citation: Langer, Gerald (2014): Experiment: Growth rates and percentages of normal versus malformed versus incomplete versus incomplete and malformed coccoliths. PANGAEA, https://doi.org/10.1594/PANGAEA.829716, Supplement to: Langer, Gerald; de Nooijer, Lennart Jan; Oetjen, Kerstin (2010): On the role of the cytoskeleton in coccolith morphogenenesis: the effect of cytoskeleton inhibitors. Journal of Phycology, 46(6), 1252-1256, https://doi.org/10.1111/j.1529-8817.2010.00916.x Abstract: The coccolithophore Emiliania huxleyi (Lohmann) W. W. Hay et H. Mohler was cultured in natural seawater with the addition of either the microtubule-inhibitor colchicine, the actin-inhibitor cytochalasin B, or the photosynthesis inhibitor 3-(3,4 dichlorophenyl)-1,1-dimethyl-urea (DCMU). Additionally, E. huxleyi was cultured at different light intensities and temperatures. Growth rate was monitored, and coccolith morphology analyzed. While every treatment affected growth rate, the percentage of malformed coccoliths increased with colchicine, cytochalasin B, and at higher than optimal temperature. These results represent the first experimental evidence for the role of microtubules and actin microfilaments in coccolith morphogenesis. Project(s): Biological Impacts of Ocean Acidification (BIOACID) (URI: https://www.bioacid.de/) Parameter(s): Variable (Variable) * PI: Langer, Gerald (https://orcid.org/0000-0002-7211-4889, gerald.langer@uab.cat) Treatment (Treat) * PI: Langer, Gerald (https://orcid.org/0000-0002-7211-4889, gerald.langer@uab.cat) Growth rate [#/day] (µ) * PI: Langer, Gerald (https://orcid.org/0000-0002-7211-4889, gerald.langer@uab.cat) Growth rate, standard deviation [±] (µ std dev) * PI: Langer, Gerald (https://orcid.org/0000-0002-7211-4889, gerald.langer@uab.cat) Coccoliths, normal [%] (Cocco norm) * PI: Langer, Gerald (https://orcid.org/0000-0002-7211-4889, gerald.langer@uab.cat) Coccoliths, normal, standard deviation [±] (Cocco norm std dev) * PI: Langer, Gerald (https://orcid.org/0000-0002-7211-4889, gerald.langer@uab.cat) Malformation rate [%] (Malformation) * PI: Langer, Gerald (https://orcid.org/0000-0002-7211-4889, gerald.langer@uab.cat) Malformation rate, standard deviation [±] (Malformation std dev) * PI: Langer, Gerald (https://orcid.org/0000-0002-7211-4889, gerald.langer@uab.cat) Coccoliths, incomplete [%] (Cocco incomplete) * PI: Langer, Gerald (https://orcid.org/0000-0002-7211-4889, gerald.langer@uab.cat) Coccoliths, incomplete, standard deviation [±] (Cocco incomplete std dev) * PI: Langer, Gerald (https://orcid.org/0000-0002-7211-4889, gerald.langer@uab.cat) Coccoliths, malformed and incomplete [%] (Cocco malformed) * PI: Langer, Gerald (https://orcid.org/0000-0002-7211-4889, gerald.langer@uab.cat) Coccoliths, malformed and incomplete, standard deviation [±] (Cocco malformed std dev) * PI: Langer, Gerald (https://orcid.org/0000-0002-7211-4889, gerald.langer@uab.cat) License: Creative Commons Attribution 3.0 Unported (CC-BY-3.0) (URI: https://creativecommons.org/licenses/by/3.0/) Size: 204 data points */ Variable Treat µ [#/day] µ std dev [±] Cocco norm [%] Cocco norm std dev [±] Malformation [%] Malformation std dev [±] Cocco incomplete [%] Cocco incomplete std dev [±] Cocco malformed [%] Cocco malformed std dev [±] Colchicine III Control 1.35 0.01 91.90 0.17 3.27 1.02 1.93 0.47 2.90 0.71 Colchicine III 500 µM 1.25 0.01 89.67 2.15 4.93 0.01 1.78 0.42 3.62 2.56 Colchicine IV Control 1.34 0.01 92.45 0.87 4.00 0.76 1.15 0.34 2.40 0.45 Colchicine IV 750 µM 0.95 0.00 81.27 1.66 8.93 2.85 2.47 1.03 7.33 0.16 Colchicine II Control 1.33 0.02 90.85 1.88 4.75 0.69 2.38 0.34 2.02 0.85 Colchicine II 1000 µM 0.59 0.04 42.25 4.10 22.09 1.54 2.53 0.14 33.14 2.69 Cytochalasin test 0.1 µM 1.15 Cytochalasin test 1 µM 1.18 Cytochalasin test 10 µM 1.02 Cytochalasin test 100 µM 0.20 Cytochalasin Control 1.51 0.03 86.99 3.96 6.33 3.38 2.42 1.50 4.25 2.08 Cytochalasin 100 µM 0.20 0.02 15.31 1.10 29.34 2.82 2.55 1.71 52.80 3.43 Photosynthesis Control 1.41 0.03 88.91 2.12 4.21 1.11 3.27 0.75 3.61 0.27 Photosynthesis DCMU, 0.01 µM 0.58 0.01 92.82 1.55 3.49 0.08 3.07 1.35 0.62 0.13 Photosynthesis Low light, 5-7 µMol photons/m**2/s 0.56 0.08 87.67 4.61 7.18 3.91 4.28 0.79 0.87 0.09 DMSO Control 1.23 0.15 DMSO 0.04vol % 1.41 0.01 DMSO 0.1vol % 1.44 0.03 DMSO 0.2vol % 1.41 0.02 DMSO 0.5vol % 1.41 0.00 Temperature 10?C 80.99 10.77 7.99 1.77 1.86 0.44 9.16 8.56 Temperature 15?C 86.87 0.27 3.39 0.53 3.43 1.19 6.31 0.39 Temperature 20?C 89.08 1.23 4.05 0.87 3.17 0.37 3.69 0.01 Temperature 25?C 28.86 1.33 24.67 2.58 1.73 1.27 44.74 2.52