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Langer, Gerald; Probert, Ian; Nehrke, Gernot; Ziveri, Patrizia (2011): The morphological response of Emiliania huxleyi to seawater carbonate chemistry changes: an inter-strain comparison [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.826456, Supplement to: Langer, G et al. (2011): The morphological response of Emiliania huxleyi to seawater carbonate chemistry changes: an inter-strain comparison. Journal of Nannoplankton Research, 32(1), 29-34, hdl:10013/epic.37875.d001

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Abstract:
Four strains of the coccolithophore Emiliania huxleyi (RCC1212, RCC1216, RCC1238, RCC1256) were grown in dilute batch culture at four CO2 levels ranging from ~200 µatm to ~1200 µatm. Coccolith morphology was analyzed based on scanning electron micrographs. Three of the four strains did not exhibit a change in morphology over the CO2 range tested. One strain (RCC1256) displayed an increase in the percentage of malformed coccoliths with increasing CO2 concentration. We conclude that the sensitivity of the coccolith-shaping machinery to carbonate chemistry changes is strain-specific. Although it has been shown before that carbonate chemistry related changes in growth- and calcification rate are strain-specific, there seems to be no consistent correlation between coccolith morphology and growth or calcification rate. We did not observe an increase in the percentage of incomplete coccoliths in RCC1256, indicating that the coccolith-shaping machinery per se is affected by acidification and not the signalling pathway that produces the stop-signal for coccolith growth.
Keyword(s):
Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Single species
Further details:
Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb
Project(s):
Biological Impacts of Ocean Acidification (BIOACID)
European Project on Ocean Acidification (EPOCA)
Mediterranean Sea Acidification in a Changing Climate (MedSeA)
Ocean Acidification International Coordination Centre (OA-ICC)
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Seventh Framework Programme (FP7), grant/award no. 265103: Mediterranean Sea Acidification in a Changing Climate
Coverage:
Median Latitude: 5.162500 * Median Longitude: 76.658333 * South-bound Latitude: -42.300000 * West-bound Longitude: -20.333330 * North-bound Latitude: 63.400000 * East-bound Longitude: 169.833330
Date/Time Start: 1998-09-01T00:00:00 * Date/Time End: 2005-11-30T00:00:00
Event(s):
North_Atlantic_OA  * Latitude: 63.400000 * Longitude: -20.333330 * Date/Time Start: 1999-07-01T00:00:00 * Date/Time End: 1999-07-31T00:00:00 * Method/Device: Experiment (EXP) * Comment: Isolation date and location of culture strains
North_Pacific_OA  * Latitude: 34.016670 * Longitude: 139.833330 * Date/Time Start: 2005-11-01T00:00:00 * Date/Time End: 2005-11-30T00:00:00 * Method/Device: Experiment (EXP) * Comment: Isolation date and location of culture strains
South_Atlantic_OA  * Latitude: -34.466670 * Longitude: 17.300000 * Date/Time Start: 2000-09-01T00:00:00 * Date/Time End: 2000-09-30T00:00:00 * Method/Device: Experiment (EXP) * Comment: Isolation date and location of culture strains
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-01-20.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 Event labelEvent
2 SpeciesSpeciesLanger, Gerald
3 StrainStrainLanger, Gerald
4 ReplicatesRepl#Langer, Gerald
5 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmLanger, GeraldCalculated using CO2SYS
6 Coccoliths, normalCocco norm%Langer, Gerald
7 Coccoliths, normal, standard deviationCocco norm std dev±Langer, Gerald
8 Malformation rateMalformation%Langer, Gerald
9 Malformation rate, standard deviationMalformation std dev±Langer, Gerald
10 Coccoliths, incompleteCocco incomplete%Langer, Gerald
11 Coccoliths, incomplete, standard deviationCocco incomplete std dev±Langer, Gerald
12 Coccoliths, malformed and incompleteCocco malformed%Langer, Gerald
13 Coccoliths, malformed and incomplete, standard deviationCocco malformed std dev±Langer, Gerald
14 Nitrate[NO3]-µmol/lLanger, Gerald
15 Phosphate[PO4]3-µmol/lLanger, Gerald
16 Alkalinity, totalATµmol/kgLanger, GeraldPotentiometric titration
17 Carbon, inorganic, dissolvedDICµmol/kgLanger, Gerald
18 pHpHLanger, GeraldCalculated using CO2SYSNBS scale
19 Bicarbonate ion[HCO3]-µmol/kgLanger, GeraldCalculated using CO2SYS
20 Carbonate ion[CO3]2-µmol/kgLanger, GeraldCalculated using CO2SYS
21 Calcite saturation stateOmega CalLanger, GeraldCalculated using CO2SYS
22 SalinitySalLanger, Gerald
23 Temperature, waterTemp°CLanger, Gerald
24 Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
25 pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
26 Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
27 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
28 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29 Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30 Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31 Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32 Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
496 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Event		2 
Species		3 
Strain		4 
Repl [#]		5 
pCO2water_SST_wet [µatm]
(Calculated using CO2SYS)		6 
Cocco norm [%]		7 
Cocco norm std dev [±]		8 
Malformation [%]		9 
Malformation std dev [±]		10 
Cocco incomplete [%]		11 
Cocco incomplete std dev [±]		12 
Cocco malformed [%]		13 
Cocco malformed std dev [±]		14 
[NO3]- [µmol/l]		15 
[PO4]3- [µmol/l]		16 
AT [µmol/kg]
(Potentiometric titration)		17 
DIC [µmol/kg]		18 
pH
(NBS scale, Calculated using C...)		19 
[HCO3]- [µmol/kg]
(Calculated using CO2SYS)		20 
[CO3]2- [µmol/kg]
(Calculated using CO2SYS)		21 
Omega Cal
(Calculated using CO2SYS)		22 
Sal		23 
Temp [°C]		24 
CSC flag
(Calculated using seacarb afte...)		25 
pH
(total scale, Calculated using...)		26 
CO2 [µmol/kg]
(Calculated using seacarb afte...)		27 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		28 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		29 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)		30 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)		31 
Omega Arg
(Calculated using seacarb afte...)		32 
Omega Cal
(Calculated using seacarb afte...)
North_Pacific_OA Emiliania huxleyi (phytoplankton)RCC1238120694.82.12.20.92.41.00.60.81006.25252220868.4517683117.632.00020.000158.32720520417693114.907.59
North_Pacific_OAEmiliania huxleyi (phytoplankton)RCC1238239593.90.71.70.43.31.31.10.81006.25230220508.1918581794.432.00020.000158.061339239118581792.834.38
North_Pacific_OAEmiliania huxleyi (phytoplankton)RCC1238368194.82.01.40.62.50.91.40.61006.25218420397.9619071102.732.00020.000157.842267567319071101.732.68
North_Pacific_OAEmiliania huxleyi (phytoplankton)RCC1238492993.31.50.50.52.81.13.41.01006.25210720137.831902802.032.00020.000157.70309259221903801.261.95
Tasman_Sea_OA Emiliania huxleyi (phytoplankton)RCC1216121896.81.71.40.71.61.10.20.31006.25248721028.4218212736.732.00017.000158.30821721618212734.266.65
Tasman_Sea_OAEmiliania huxleyi (phytoplankton)RCC1216242297.60.10.60.21.50.30.20.21006.25228120678.1618981533.732.00017.000158.031542041818991532.393.73
Tasman_Sea_OAEmiliania huxleyi (phytoplankton)RCC1216372997.80.10.60.41.50.20.10.21006.25217720607.931941932.332.00017.000157.81267267231941931.452.26
Tasman_Sea_OAEmiliania huxleyi (phytoplankton)RCC12164120192.24.23.11.51.81.02.91.71006.25207520297.711930561.432.00017.000157.5843120211981931550.861.35
South_Atlantic_OA Emiliania huxleyi (phytoplankton)RCC1212119451.05.042.85.24.00.82.32.31006.25251720678.4717413197.832.00020.000158.34619319217413205.057.82
South_Atlantic_OAEmiliania huxleyi (phytoplankton)RCC1212240958.52.935.71.64.01.91.80.51006.25231320668.1818771764.332.00020.000158.051340740518761762.784.31
South_Atlantic_OAEmiliania huxleyi (phytoplankton)RCC1212375266.03.527.54.34.00.62.60.31006.25220320717.9319441032.532.00020.000157.802574874619441031.622.51
South_Atlantic_OAEmiliania huxleyi (phytoplankton)RCC12124109665.25.628.24.42.20.54.41.61006.25212820537.771947711.732.00020.000157.6436109010861946711.121.73
North_Atlantic_OA Emiliania huxleyi (phytoplankton)RCC1256119397.01.11.20.21.50.90.20.41006.25245620498.4617562867.032.00017.000158.34719219117552874.486.98
North_Atlantic_OAEmiliania huxleyi (phytoplankton)RCC1256239997.30.61.00.41.60.70.20.21006.25224020228.1718531553.832.00017.000158.051439739518531542.413.76
North_Atlantic_OAEmiliania huxleyi (phytoplankton)RCC1256358791.30.87.01.20.70.21.00.31006.25213619938.0018651062.632.00017.000157.882158558318661061.662.58
North_Atlantic_OAEmiliania huxleyi (phytoplankton)RCC1256491561.02.529.41.51.41.18.21.81006.25205119747.811872691.732.00017.000157.69339119081873691.071.67