Citation:

journal.pone.0065987

Name: Seawater carbonate chemistry and growth, calcification of Thoracosphaera heimii in a laboratory experiment
Published: 2013-12-18
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Myzozoa; Not applicable; Pelagos; Phytoplankton; Single species; Thoracosphaera heimii

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Abstract:

Ocean acidification is considered a major threat to marine ecosystems and may particularly affect calcifying organisms such as corals, foraminifera and coccolithophores. Here we investigate the impact of elevated pCO2 and lowered pH on growth and calcification in the common calcareous dinoflagellate Thoracosphaera heimii. We observe a substantial reduction in growth rate, calcification and cyst stability of T. heimii under elevated pCO2. Furthermore, transcriptomic analyses reveal CO2 sensitive regulation of many genes, particularly those being associated to inorganic carbon acquisition and calcification. Stable carbon isotope fractionation for organic carbon production increased with increasing pCO2 whereas it decreased for calcification, which suggests interdependence between both processes. We also found a strong effect of pCO2 on the stable oxygen isotopic composition of calcite, in line with earlier observations concerning another T. heimii strain. The observed changes in stable oxygen and carbon isotope composition of T. heimii cysts may provide an ideal tool for reconstructing past seawater carbonate chemistry, and ultimately past pCO2. Although the function of calcification in T. heimii remains unresolved, this trait likely plays an important role in the ecological and evolutionary success of this species. Acting on calcification as well as growth, ocean acidification may therefore impose a great threat for T. heimii.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Myzozoa; Not applicable; Pelagos; Phytoplankton; Single species; Thoracosphaera heimii

Original version:

Van de Waal, Dedmer B; John, Uwe; Ziveri, Patrizia; Reichart, Gert-Jan; Hoins, Mirja; Sluijs, Appy; Rost, Björn (2013): General responses of Thoracosphaera heimii grown under a range of pCO2. PANGAEA, https://doi.org/10.1594/PANGAEA.816429

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb

Project(s):

Mediterranean Sea Acidification in a Changing Climate (MedSeA)

Ocean Acidification International Coordination Centre (OA-ICC)

Funding:

Seventh Framework Programme (FP7), grant/award no. 265103 : Mediterranean Sea Acidification in a Changing Climate

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-12-16.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Identification	ID		Van de Waal, Dedmer B
2	Species	Species		Van de Waal, Dedmer B
3	Treatment	Treat		Van de Waal, Dedmer B
4	Replicates	Repl	#	Van de Waal, Dedmer B
5	Carbon dioxide	CO2	µmol/l	Van de Waal, Dedmer B
6	Growth rate	µ	1/day	Van de Waal, Dedmer B
7	Particulate organic carbon production per cell	POC prod/cell	pg/#	Van de Waal, Dedmer B
8	Particulate inorganic carbon production per cell	PIC prod/cell	pg/#	Van de Waal, Dedmer B
9	Particulate inorganic carbon/particulate organic carbon ratio	PIC/POC		Van de Waal, Dedmer B	Calculated
10	Cysts	Cysts	%	Van de Waal, Dedmer B		completed
11	Fractionation of calcite	εk	‰	Van de Waal, Dedmer B
12	Fractionation of organic carbon	εp Corg	‰	Van de Waal, Dedmer B
13	δ18O, calcite	δ18O cal	‰ PDB	Van de Waal, Dedmer B
14	δ18O, dissolved inorganic carbon	δ18O DIC	‰	Van de Waal, Dedmer B
15	δ18O, calcite	δ18O cal	‰ SMOW	Van de Waal, Dedmer B		calcite-water
16	Carbonate ion	[CO3]2-	µmol/l	Van de Waal, Dedmer B
17	Protein name	Protein		Van de Waal, Dedmer B
18	Gene expression	Gene expression		Van de Waal, Dedmer B		treatment/present day
19	Incubation duration	Inc dur	days	Van de Waal, Dedmer B
20	Abundance per volume	Abund v	#/ml	Van de Waal, Dedmer B		vegetative cysts/mL
21	Category	Cat		Van de Waal, Dedmer B		KOG
22	Gene expression	Gene expression		Van de Waal, Dedmer B		distribution
23	Salinity	Sal		Van de Waal, Dedmer B
24	Temperature, water	Temp	°C	Van de Waal, Dedmer B
25	Partial pressure of carbon dioxide (water) at equilibrator temperature (wet air)	pCO2water_equ_wet	µatm	Van de Waal, Dedmer B	Calculated using CO2SYS	start
26	pH	pH		Van de Waal, Dedmer B	Potentiometric	start, NBS scale
27	Carbon, inorganic, dissolved	DIC	µmol/kg	Van de Waal, Dedmer B	Calculated using CO2SYS	start
28	Carbon dioxide	CO2	µmol/kg	Van de Waal, Dedmer B	Calculated using CO2SYS	start
29	Alkalinity, total	AT	µmol/kg	Van de Waal, Dedmer B	Potentiometric titration	start
30	Calcite saturation state	Omega Cal		Van de Waal, Dedmer B	Calculated using CO2SYS	start
31	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	start
32	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	start, total scale
33	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	start
34	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	start
35	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	start
36	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	start
37	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	start
38	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	start
39	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	start
40	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	start
41	Partial pressure of carbon dioxide (water) at equilibrator temperature (wet air)	pCO2water_equ_wet	µatm	Van de Waal, Dedmer B	Calculated using CO2SYS	end
42	pH	pH		Van de Waal, Dedmer B	Potentiometric	end, NBS scale
43	Carbon, inorganic, dissolved	DIC	µmol/kg	Van de Waal, Dedmer B	Calculated using CO2SYS	end
44	Carbon dioxide	CO2	µmol/kg	Van de Waal, Dedmer B	Calculated using CO2SYS	end
45	Alkalinity, total	AT	µmol/kg	Van de Waal, Dedmer B	Potentiometric titration	end
46	Calcite saturation state	Omega Cal		Van de Waal, Dedmer B	Calculated using CO2SYS	end
47	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	end
48	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	end, total scale
49	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	end
50	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	end
51	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	end
52	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	end
53	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	end
54	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	end
55	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	end
56	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	end

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

8222 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 ID	2 Species	3 Treat	4 Repl [#]	5 CO2 [µmol/l]	6 µ [1/day]	7 POC prod/cell [pg/#]	8 PIC prod/cell [pg/#]	9 PIC/POC (Calculated)	10 Cysts [%] (completed)	11 εk [‰]	12 εp Corg [‰]	13 δ18O cal [‰ PDB]	14 δ18O DIC [‰]	15 δ18O cal [‰ SMOW] (calcite-water)	16 [CO3]2- [µmol/l]	17 Protein	18 Gene expression (treatment/present day)	19 Inc dur [days]	20 Abund v [#/ml] (vegetative cysts/mL)	21 Cat (KOG)	22 Gene expression (distribution)	23 Sal	24 Temp [°C]	25 pCO2water_equ_wet [µatm] (start, Calculated using CO2SYS)	26 pH (start, NBS scale, Potentiometric)	27 DIC [µmol/kg] (start, Calculated using CO2SYS)	28 CO2 [µmol/kg] (start, Calculated using CO2SYS)	29 AT [µmol/kg] (start, Potentiometric titration)	30 Omega Cal (start, Calculated using CO2SYS)	31 CSC flag (start, Calculated using seaca...)	32 pH (start, total scale, Calculate...)	33 CO2 [µmol/kg] (start, Calculated using seaca...)	34 pCO2water_SST_wet [µatm] (start, Calculated using seaca...)	35 fCO2water_SST_wet [µatm] (start, Calculated using seaca...)	36 [HCO3]- [µmol/kg] (start, Calculated using seaca...)	37 [CO3]2- [µmol/kg] (start, Calculated using seaca...)	38 DIC [µmol/kg] (start, Calculated using seaca...)	39 Omega Arg (start, Calculated using seaca...)	40 Omega Cal (start, Calculated using seaca...)	41 pCO2water_equ_wet [µatm] (end, Calculated using CO2SYS)	42 pH (end, NBS scale, Potentiometric)	43 DIC [µmol/kg] (end, Calculated using CO2SYS)	44 CO2 [µmol/kg] (end, Calculated using CO2SYS)	45 AT [µmol/kg] (end, Potentiometric titration)	46 Omega Cal (end, Calculated using CO2SYS)	47 CSC flag (end, Calculated using seacarb...)	48 pH (end, total scale, Calculated ...)	49 CO2 [µmol/kg] (end, Calculated using seacarb...)	50 pCO2water_SST_wet [µatm] (end, Calculated using seacarb...)	51 fCO2water_SST_wet [µatm] (end, Calculated using seacarb...)	52 [HCO3]- [µmol/kg] (end, Calculated using seacarb...)	53 [CO3]2- [µmol/kg] (end, Calculated using seacarb...)	54 DIC [µmol/kg] (end, Calculated using seacarb...)	55 Omega Arg (end, Calculated using seacarb...)	56 Omega Cal (end, Calculated using seacarb...)
Figure 1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	1	9	0.35	297	58	0.19	98													34	15	150	8.52	1990	5.8	2443	7.27	26	8.41	6	154	154	1683	311	2000	4.79	7.47	251	8.34	2068	9.6	2392	5.24	26	8.22	10	257	256	1843	224	2076	3.45	5.37
Figure 1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	2	9	0.42	282	77	0.27	96													34	15	150	8.52	1979	5.8	2429	7.22	26	8.41	6	154	153	1674	309	1988	4.75	7.41	238	8.35	2023	9.1	2351	5.25	26	8.24	9	244	243	1798	225	2032	3.46	5.39
Figure 1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	3	9	0.29	301	79	0.26	98													34	15	152	8.52	1977	5.8	2422	7.14	26	8.40	6	156	155	1674	306	1986	4.70	7.33	241	8.35	2031	9.3	2357	5.23	26	8.23	9	248	247	1807	224	2040	3.44	5.37
Figure 1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	1	19	0.29	316	88	0.28	97													34	15	326	8.25	2125	12.5	2396	4.43	26	8.13	13	335	334	1932	189	2134	2.91	4.54	496	8.08	2166	19.1	2354	3.18	26	7.97	19	508	507	2019	136	2174	2.09	3.27
Figure 1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	2	19	0.32	302	99	0.33	90													34	15	332	8.24	2129	12.8	2396	4.37	26	8.12	13	341	340	1938	187	2137	2.88	4.49	508	8.07	2173	19.5	2357	3.13	26	7.96	20	520	518	2027	134	2181	2.06	3.22
Figure 1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	3	19	0.31	279	89	0.32	97													34	15	332	8.24	2126	12.8	2393	4.36	26	8.12	13	340	339	1935	187	2134	2.87	4.48	513	8.08	2203	19.7	2390	3.19	26	7.96	20	526	524	2055	137	2211	2.10	3.27
Figure 1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	1	35	0.24	378	69	0.18	64													34	15	627	8.00	2251	24.1	2406	2.77	26	7.89	24	643	641	2116	118	2259	1.82	2.84	933	7.84	2287	35.9	2380	1.96	26	7.73	36	958	955	2174	84	2294	1.29	2.01
Figure 1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	2	34	0.27	320	51	0.16	66													34	15	624	8.00	2218	24.0	2371	2.70	26	7.88	24	638	636	2086	116	2226	1.78	2.78	915	7.85	2269	35.2	2364	1.97	26	7.73	35	936	933	2157	84	2276	1.30	2.02
Figure 1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	3	34	0.30	262	58	0.22	73													34	15	619	8.00	2226	23.8	2380	2.74	26	7.89	24	636	634	2093	117	2234	1.80	2.81	895	7.86	2303	34.4	2404	2.07	26	7.75	34	918	915	2187	88	2310	1.36	2.12
Figure 1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	1	64	0.14	363	36	0.10	17													34	15	1171	7.74	2284	45.0	2345	1.57	26	7.63	45	1200	1195	2179	67	2292	1.03	1.61	1711	7.59	2363	65.8	2376	1.15	26	7.48	66	1749	1743	2255	49	2370	0.76	1.18
Figure 1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	2	65	0.18	337	39	0.11	21													34	15	1224	7.73	2291	47.0	2346	1.51	26	7.61	47	1252	1247	2186	65	2298	1.00	1.56	1721	7.59	2382	66.1	2395	1.16	26	7.48	66	1765	1759	2273	50	2389	0.76	1.19
Figure 1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	3	65	0.15	327	35	0.11	23													34	15	1214	7.73	2298	46.7	2354	1.54	26	7.61	47	1247	1243	2193	65	2305	1.01	1.57	1727	7.59	2369	66.4	2381	1.15	26	7.48	66	1771	1765	2261	49	2376	0.75	1.18
Figure 3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	1	7						8	11	-5.95	1.13	-5.35	260							34	15	150	8.52	1990	5.8	2443	7.27	26	8.41	6	154	154	1683	311	2000	4.79	7.47	251	8.34	2068	9.6	2392	5.24	26	8.22	10	257	256	1843	224	2076	3.45	5.37
Figure 3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	2	7						10	9	-5.67	1.25	-5.15	255							34	15	150	8.52	1979	5.8	2429	7.22	26	8.41	6	154	153	1674	309	1988	4.75	7.41	238	8.35	2023	9.1	2351	5.25	26	8.24	9	244	243	1798	225	2032	3.46	5.39
Figure 3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	3	7						12	9	-5.82	1.23	-5.31	263							34	15	152	8.52	1977	5.8	2422	7.14	26	8.40	6	156	155	1674	306	1986	4.70	7.33	241	8.35	2031	9.3	2357	5.23	26	8.23	9	248	247	1807	224	2040	3.44	5.37
Figure 3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	1	18						11	8	-3.00	1.66	-2.32	141							34	15	326	8.25	2125	12.5	2396	4.43	26	8.13	13	335	334	1932	189	2134	2.91	4.54	496	8.08	2166	19.1	2354	3.18	26	7.97	19	508	507	2019	136	2174	2.09	3.27
Figure 3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	2	18						12	9	-3.11	1.89	-2.65	142							34	15	332	8.24	2129	12.8	2396	4.37	26	8.12	13	341	340	1938	187	2137	2.88	4.49	508	8.07	2173	19.5	2357	3.13	26	7.96	20	520	518	2027	134	2181	2.06	3.22
Figure 3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	3	18						12	9	-2.96	1.94	-2.56	144							34	15	332	8.24	2126	12.8	2393	4.36	26	8.12	13	340	339	1935	187	2134	2.87	4.48	513	8.08	2203	19.7	2390	3.19	26	7.96	20	526	524	2055	137	2211	2.10	3.27
Figure 3	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	1	33						12	4	-1.56	2.04	-0.98	87							34	15	627	8.00	2251	24.1	2406	2.77	26	7.89	24	643	641	2116	118	2259	1.82	2.84	933	7.84	2287	35.9	2380	1.96	26	7.73	36	958	955	2174	84	2294	1.29	2.01
Figure 3	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	2	33						14	2	-1.18	2.14	-0.70	86							34	15	624	8.00	2218	24.0	2371	2.70	26	7.88	24	638	636	2086	116	2226	1.78	2.78	915	7.85	2269	35.2	2364	1.97	26	7.73	35	936	933	2157	84	2276	1.30	2.02
Figure 3	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	3	33						15	3	-1.52	2.12	-1.02	86							34	15	619	8.00	2226	23.8	2380	2.74	26	7.89	24	636	634	2093	117	2234	1.80	2.81	895	7.86	2303	34.4	2404	2.07	26	7.75	34	918	915	2187	88	2310	1.36	2.12
Figure 3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	1	70						17	0	-0.43	2.34	0.10	46							34	15	1171	7.74	2284	45.0	2345	1.57	26	7.63	45	1200	1195	2179	67	2292	1.03	1.61	1711	7.59	2363	65.8	2376	1.15	26	7.48	66	1749	1743	2255	49	2370	0.76	1.18
Figure 3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	2	71						17	1	-0.49	2.37	0.02	45							34	15	1224	7.73	2291	47.0	2346	1.51	26	7.61	47	1252	1247	2186	65	2298	1.00	1.56	1721	7.59	2382	66.1	2395	1.16	26	7.48	66	1765	1759	2273	50	2389	0.76	1.19
Figure 3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	5	72						15	0	-0.47	2.40	0.02	44							34	15	1214	7.73	2298	46.7	2354	1.54	26	7.61	47	1247	1243	2193	65	2305	1.01	1.57	1727	7.59	2369	66.4	2381	1.15	26	7.48	66	1771	1765	2261	49	2376	0.75	1.18
Figure 4	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm														P-ATPase	0.06					34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure 4	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm														NCX	0.15					34	15	151	8.52	1980	5.8	2429	7.20	26	8.40	6	155	154	1676	308	1990	4.74	7.39	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure 4	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm														VCX	0.63					34	15	151	8.52	1981	5.8	2429	7.20	26	8.40	6	155	154	1676	308	1990	4.73	7.39	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure 4	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm														V-ATPase	1.09					34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure 4	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm														CA	0.25					34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure 4	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm														Aquaporin	0.67					34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure 4	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm														AE	0.00					34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure 4	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm														SAT-1	0.00					34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure 4	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm														P-ATPase	0.12					34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure 4	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm														NCX	2.31					34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure 4	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm														VCX	1.11					34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure 4	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm														V-ATPase	2.86					34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure 4	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm														CA	2.20					34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure 4	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm														Aquaporin	5.33					34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure 4	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm														AE	0.00					34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure 4	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm														SAT-1	0.00					34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	1															2	129			34	15	150	8.52	1990	5.8	2443	7.27	26	8.41	6	154	154	1683	311	2000	4.79	7.47	251	8.34	2068	9.6	2392	5.24	26	8.22	10	257	256	1843	224	2076	3.45	5.37
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	1															2	122			34	15	150	8.52	1990	5.8	2443	7.27	26	8.41	6	154	154	1683	311	2000	4.79	7.47	251	8.34	2068	9.6	2392	5.24	26	8.22	10	257	256	1843	224	2076	3.45	5.37
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	1															4	239			34	15	150	8.52	1990	5.8	2443	7.27	26	8.41	6	154	154	1683	311	2000	4.79	7.47	251	8.34	2068	9.6	2392	5.24	26	8.22	10	257	256	1843	224	2076	3.45	5.37
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	1															4	280			34	15	150	8.52	1990	5.8	2443	7.27	26	8.41	6	154	154	1683	311	2000	4.79	7.47	251	8.34	2068	9.6	2392	5.24	26	8.22	10	257	256	1843	224	2076	3.45	5.37
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	1															7	515			34	15	150	8.52	1990	5.8	2443	7.27	26	8.41	6	154	154	1683	311	2000	4.79	7.47	251	8.34	2068	9.6	2392	5.24	26	8.22	10	257	256	1843	224	2076	3.45	5.37
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	1															8	905			34	15	150	8.52	1990	5.8	2443	7.27	26	8.41	6	154	154	1683	311	2000	4.79	7.47	251	8.34	2068	9.6	2392	5.24	26	8.22	10	257	256	1843	224	2076	3.45	5.37
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	1															8	950			34	15	150	8.52	1990	5.8	2443	7.27	26	8.41	6	154	154	1683	311	2000	4.79	7.47	251	8.34	2068	9.6	2392	5.24	26	8.22	10	257	256	1843	224	2076	3.45	5.37
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	1															8	940			34	15	150	8.52	1990	5.8	2443	7.27	26	8.41	6	154	154	1683	311	2000	4.79	7.47	251	8.34	2068	9.6	2392	5.24	26	8.22	10	257	256	1843	224	2076	3.45	5.37
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	2															2	98			34	15	150	8.52	1979	5.8	2429	7.22	26	8.41	6	154	153	1674	309	1988	4.75	7.41	238	8.35	2023	9.1	2351	5.25	26	8.24	9	244	243	1798	225	2032	3.46	5.39
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	2															2	94			34	15	150	8.52	1979	5.8	2429	7.22	26	8.41	6	154	153	1674	309	1988	4.75	7.41	238	8.35	2023	9.1	2351	5.25	26	8.24	9	244	243	1798	225	2032	3.46	5.39
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	2															4	189			34	15	150	8.52	1979	5.8	2429	7.22	26	8.41	6	154	153	1674	309	1988	4.75	7.41	238	8.35	2023	9.1	2351	5.25	26	8.24	9	244	243	1798	225	2032	3.46	5.39
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	2															4	245			34	15	150	8.52	1979	5.8	2429	7.22	26	8.41	6	154	153	1674	309	1988	4.75	7.41	238	8.35	2023	9.1	2351	5.25	26	8.24	9	244	243	1798	225	2032	3.46	5.39
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	2															7	585			34	15	150	8.52	1979	5.8	2429	7.22	26	8.41	6	154	153	1674	309	1988	4.75	7.41	238	8.35	2023	9.1	2351	5.25	26	8.24	9	244	243	1798	225	2032	3.46	5.39
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	2															8	855			34	15	150	8.52	1979	5.8	2429	7.22	26	8.41	6	154	153	1674	309	1988	4.75	7.41	238	8.35	2023	9.1	2351	5.25	26	8.24	9	244	243	1798	225	2032	3.46	5.39
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	2															8	1070			34	15	150	8.52	1979	5.8	2429	7.22	26	8.41	6	154	153	1674	309	1988	4.75	7.41	238	8.35	2023	9.1	2351	5.25	26	8.24	9	244	243	1798	225	2032	3.46	5.39
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	2															8	1185			34	15	150	8.52	1979	5.8	2429	7.22	26	8.41	6	154	153	1674	309	1988	4.75	7.41	238	8.35	2023	9.1	2351	5.25	26	8.24	9	244	243	1798	225	2032	3.46	5.39
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	3															2	122			34	15	152	8.52	1977	5.8	2422	7.14	26	8.40	6	156	155	1674	306	1986	4.70	7.33	241	8.35	2031	9.3	2357	5.23	26	8.23	9	248	247	1807	224	2040	3.44	5.37
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	3															2	122			34	15	152	8.52	1977	5.8	2422	7.14	26	8.40	6	156	155	1674	306	1986	4.70	7.33	241	8.35	2031	9.3	2357	5.23	26	8.23	9	248	247	1807	224	2040	3.44	5.37
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	3															4	232			34	15	152	8.52	1977	5.8	2422	7.14	26	8.40	6	156	155	1674	306	1986	4.70	7.33	241	8.35	2031	9.3	2357	5.23	26	8.23	9	248	247	1807	224	2040	3.44	5.37
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	3															4	305			34	15	152	8.52	1977	5.8	2422	7.14	26	8.40	6	156	155	1674	306	1986	4.70	7.33	241	8.35	2031	9.3	2357	5.23	26	8.23	9	248	247	1807	224	2040	3.44	5.37
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	3															7	675			34	15	152	8.52	1977	5.8	2422	7.14	26	8.40	6	156	155	1674	306	1986	4.70	7.33	241	8.35	2031	9.3	2357	5.23	26	8.23	9	248	247	1807	224	2040	3.44	5.37
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	3															8	847			34	15	152	8.52	1977	5.8	2422	7.14	26	8.40	6	156	155	1674	306	1986	4.70	7.33	241	8.35	2031	9.3	2357	5.23	26	8.23	9	248	247	1807	224	2040	3.44	5.37
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	3															8	805			34	15	152	8.52	1977	5.8	2422	7.14	26	8.40	6	156	155	1674	306	1986	4.70	7.33	241	8.35	2031	9.3	2357	5.23	26	8.23	9	248	247	1807	224	2040	3.44	5.37
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm	3															8	835			34	15	152	8.52	1977	5.8	2422	7.14	26	8.40	6	156	155	1674	306	1986	4.70	7.33	241	8.35	2031	9.3	2357	5.23	26	8.23	9	248	247	1807	224	2040	3.44	5.37
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	1															2	151			34	15	326	8.25	2125	12.5	2396	4.43	26	8.13	13	335	334	1932	189	2134	2.91	4.54	496	8.08	2166	19.1	2354	3.18	26	7.97	19	508	507	2019	136	2174	2.09	3.27
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	1															2	180			34	15	326	8.25	2125	12.5	2396	4.43	26	8.13	13	335	334	1932	189	2134	2.91	4.54	496	8.08	2166	19.1	2354	3.18	26	7.97	19	508	507	2019	136	2174	2.09	3.27
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	1															4	256			34	15	326	8.25	2125	12.5	2396	4.43	26	8.13	13	335	334	1932	189	2134	2.91	4.54	496	8.08	2166	19.1	2354	3.18	26	7.97	19	508	507	2019	136	2174	2.09	3.27
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	1															4	325			34	15	326	8.25	2125	12.5	2396	4.43	26	8.13	13	335	334	1932	189	2134	2.91	4.54	496	8.08	2166	19.1	2354	3.18	26	7.97	19	508	507	2019	136	2174	2.09	3.27
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	1															7	610			34	15	326	8.25	2125	12.5	2396	4.43	26	8.13	13	335	334	1932	189	2134	2.91	4.54	496	8.08	2166	19.1	2354	3.18	26	7.97	19	508	507	2019	136	2174	2.09	3.27
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	1															8	1010			34	15	326	8.25	2125	12.5	2396	4.43	26	8.13	13	335	334	1932	189	2134	2.91	4.54	496	8.08	2166	19.1	2354	3.18	26	7.97	19	508	507	2019	136	2174	2.09	3.27
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	1															8	875			34	15	326	8.25	2125	12.5	2396	4.43	26	8.13	13	335	334	1932	189	2134	2.91	4.54	496	8.08	2166	19.1	2354	3.18	26	7.97	19	508	507	2019	136	2174	2.09	3.27
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	1															8	880			34	15	326	8.25	2125	12.5	2396	4.43	26	8.13	13	335	334	1932	189	2134	2.91	4.54	496	8.08	2166	19.1	2354	3.18	26	7.97	19	508	507	2019	136	2174	2.09	3.27
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	2															2	146			34	15	332	8.24	2129	12.8	2396	4.37	26	8.12	13	341	340	1938	187	2137	2.88	4.49	508	8.07	2173	19.5	2357	3.13	26	7.96	20	520	518	2027	134	2181	2.06	3.22
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	2															2	105			34	15	332	8.24	2129	12.8	2396	4.37	26	8.12	13	341	340	1938	187	2137	2.88	4.49	508	8.07	2173	19.5	2357	3.13	26	7.96	20	520	518	2027	134	2181	2.06	3.22
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	2															4	242			34	15	332	8.24	2129	12.8	2396	4.37	26	8.12	13	341	340	1938	187	2137	2.88	4.49	508	8.07	2173	19.5	2357	3.13	26	7.96	20	520	518	2027	134	2181	2.06	3.22
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	2															4	250			34	15	332	8.24	2129	12.8	2396	4.37	26	8.12	13	341	340	1938	187	2137	2.88	4.49	508	8.07	2173	19.5	2357	3.13	26	7.96	20	520	518	2027	134	2181	2.06	3.22
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	2															7	790			34	15	332	8.24	2129	12.8	2396	4.37	26	8.12	13	341	340	1938	187	2137	2.88	4.49	508	8.07	2173	19.5	2357	3.13	26	7.96	20	520	518	2027	134	2181	2.06	3.22
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	2															8	1040			34	15	332	8.24	2129	12.8	2396	4.37	26	8.12	13	341	340	1938	187	2137	2.88	4.49	508	8.07	2173	19.5	2357	3.13	26	7.96	20	520	518	2027	134	2181	2.06	3.22
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	2															8	910			34	15	332	8.24	2129	12.8	2396	4.37	26	8.12	13	341	340	1938	187	2137	2.88	4.49	508	8.07	2173	19.5	2357	3.13	26	7.96	20	520	518	2027	134	2181	2.06	3.22
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	2															8	890			34	15	332	8.24	2129	12.8	2396	4.37	26	8.12	13	341	340	1938	187	2137	2.88	4.49	508	8.07	2173	19.5	2357	3.13	26	7.96	20	520	518	2027	134	2181	2.06	3.22
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	3															2	132			34	15	332	8.24	2126	12.8	2393	4.36	26	8.12	13	340	339	1935	187	2134	2.87	4.48	513	8.08	2203	19.7	2390	3.19	26	7.96	20	526	524	2055	137	2211	2.10	3.27
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	3															2	210			34	15	332	8.24	2126	12.8	2393	4.36	26	8.12	13	340	339	1935	187	2134	2.87	4.48	513	8.08	2203	19.7	2390	3.19	26	7.96	20	526	524	2055	137	2211	2.10	3.27
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	3															4	244			34	15	332	8.24	2126	12.8	2393	4.36	26	8.12	13	340	339	1935	187	2134	2.87	4.48	513	8.08	2203	19.7	2390	3.19	26	7.96	20	526	524	2055	137	2211	2.10	3.27
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	3															4	270			34	15	332	8.24	2126	12.8	2393	4.36	26	8.12	13	340	339	1935	187	2134	2.87	4.48	513	8.08	2203	19.7	2390	3.19	26	7.96	20	526	524	2055	137	2211	2.10	3.27
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	3															7	710			34	15	332	8.24	2126	12.8	2393	4.36	26	8.12	13	340	339	1935	187	2134	2.87	4.48	513	8.08	2203	19.7	2390	3.19	26	7.96	20	526	524	2055	137	2211	2.10	3.27
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	3															8	1107			34	15	332	8.24	2126	12.8	2393	4.36	26	8.12	13	340	339	1935	187	2134	2.87	4.48	513	8.08	2203	19.7	2390	3.19	26	7.96	20	526	524	2055	137	2211	2.10	3.27
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	3															8	1000			34	15	332	8.24	2126	12.8	2393	4.36	26	8.12	13	340	339	1935	187	2134	2.87	4.48	513	8.08	2203	19.7	2390	3.19	26	7.96	20	526	524	2055	137	2211	2.10	3.27
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm	3															8	770			34	15	332	8.24	2126	12.8	2393	4.36	26	8.12	13	340	339	1935	187	2134	2.87	4.48	513	8.08	2203	19.7	2390	3.19	26	7.96	20	526	524	2055	137	2211	2.10	3.27
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	1															2	170			34	15	627	8.00	2251	24.1	2406	2.77	26	7.89	24	643	641	2116	118	2259	1.82	2.84	933	7.84	2287	35.9	2380	1.96	26	7.73	36	958	955	2174	84	2294	1.29	2.01
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	1															2	210			34	15	627	8.00	2251	24.1	2406	2.77	26	7.89	24	643	641	2116	118	2259	1.82	2.84	933	7.84	2287	35.9	2380	1.96	26	7.73	36	958	955	2174	84	2294	1.29	2.01
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	1															4	324			34	15	627	8.00	2251	24.1	2406	2.77	26	7.89	24	643	641	2116	118	2259	1.82	2.84	933	7.84	2287	35.9	2380	1.96	26	7.73	36	958	955	2174	84	2294	1.29	2.01
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	1															4	285			34	15	627	8.00	2251	24.1	2406	2.77	26	7.89	24	643	641	2116	118	2259	1.82	2.84	933	7.84	2287	35.9	2380	1.96	26	7.73	36	958	955	2174	84	2294	1.29	2.01
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	1															7	925			34	15	627	8.00	2251	24.1	2406	2.77	26	7.89	24	643	641	2116	118	2259	1.82	2.84	933	7.84	2287	35.9	2380	1.96	26	7.73	36	958	955	2174	84	2294	1.29	2.01
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	1															8				34	15	627	8.00	2251	24.1	2406	2.77	26	7.89	24	643	641	2116	118	2259	1.82	2.84	933	7.84	2287	35.9	2380	1.96	26	7.73	36	958	955	2174	84	2294	1.29	2.01
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	1															8	765			34	15	627	8.00	2251	24.1	2406	2.77	26	7.89	24	643	641	2116	118	2259	1.82	2.84	933	7.84	2287	35.9	2380	1.96	26	7.73	36	958	955	2174	84	2294	1.29	2.01
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	1															8	845			34	15	627	8.00	2251	24.1	2406	2.77	26	7.89	24	643	641	2116	118	2259	1.82	2.84	933	7.84	2287	35.9	2380	1.96	26	7.73	36	958	955	2174	84	2294	1.29	2.01
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	2															2	153			34	15	624	8.00	2218	24.0	2371	2.70	26	7.88	24	638	636	2086	116	2226	1.78	2.78	915	7.85	2269	35.2	2364	1.97	26	7.73	35	936	933	2157	84	2276	1.30	2.02
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	2															2	210			34	15	624	8.00	2218	24.0	2371	2.70	26	7.88	24	638	636	2086	116	2226	1.78	2.78	915	7.85	2269	35.2	2364	1.97	26	7.73	35	936	933	2157	84	2276	1.30	2.02
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	2															4	286			34	15	624	8.00	2218	24.0	2371	2.70	26	7.88	24	638	636	2086	116	2226	1.78	2.78	915	7.85	2269	35.2	2364	1.97	26	7.73	35	936	933	2157	84	2276	1.30	2.02
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	2															4	375			34	15	624	8.00	2218	24.0	2371	2.70	26	7.88	24	638	636	2086	116	2226	1.78	2.78	915	7.85	2269	35.2	2364	1.97	26	7.73	35	936	933	2157	84	2276	1.30	2.02
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	2															7	850			34	15	624	8.00	2218	24.0	2371	2.70	26	7.88	24	638	636	2086	116	2226	1.78	2.78	915	7.85	2269	35.2	2364	1.97	26	7.73	35	936	933	2157	84	2276	1.30	2.02
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	2															8	907			34	15	624	8.00	2218	24.0	2371	2.70	26	7.88	24	638	636	2086	116	2226	1.78	2.78	915	7.85	2269	35.2	2364	1.97	26	7.73	35	936	933	2157	84	2276	1.30	2.02
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	2															8	985			34	15	624	8.00	2218	24.0	2371	2.70	26	7.88	24	638	636	2086	116	2226	1.78	2.78	915	7.85	2269	35.2	2364	1.97	26	7.73	35	936	933	2157	84	2276	1.30	2.02
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	2															8	1115			34	15	624	8.00	2218	24.0	2371	2.70	26	7.88	24	638	636	2086	116	2226	1.78	2.78	915	7.85	2269	35.2	2364	1.97	26	7.73	35	936	933	2157	84	2276	1.30	2.02
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	3															2	159			34	15	619	8.00	2226	23.8	2380	2.74	26	7.89	24	636	634	2093	117	2234	1.80	2.81	895	7.86	2303	34.4	2404	2.07	26	7.75	34	918	915	2187	88	2310	1.36	2.12
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	3															2	220			34	15	619	8.00	2226	23.8	2380	2.74	26	7.89	24	636	634	2093	117	2234	1.80	2.81	895	7.86	2303	34.4	2404	2.07	26	7.75	34	918	915	2187	88	2310	1.36	2.12
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	3															4	306			34	15	619	8.00	2226	23.8	2380	2.74	26	7.89	24	636	634	2093	117	2234	1.80	2.81	895	7.86	2303	34.4	2404	2.07	26	7.75	34	918	915	2187	88	2310	1.36	2.12
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	3															4	375			34	15	619	8.00	2226	23.8	2380	2.74	26	7.89	24	636	634	2093	117	2234	1.80	2.81	895	7.86	2303	34.4	2404	2.07	26	7.75	34	918	915	2187	88	2310	1.36	2.12
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	3															7	815			34	15	619	8.00	2226	23.8	2380	2.74	26	7.89	24	636	634	2093	117	2234	1.80	2.81	895	7.86	2303	34.4	2404	2.07	26	7.75	34	918	915	2187	88	2310	1.36	2.12
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	3															8	1107			34	15	619	8.00	2226	23.8	2380	2.74	26	7.89	24	636	634	2093	117	2234	1.80	2.81	895	7.86	2303	34.4	2404	2.07	26	7.75	34	918	915	2187	88	2310	1.36	2.12
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	3															8	1110			34	15	619	8.00	2226	23.8	2380	2.74	26	7.89	24	636	634	2093	117	2234	1.80	2.81	895	7.86	2303	34.4	2404	2.07	26	7.75	34	918	915	2187	88	2310	1.36	2.12
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=750 micro atm	3															8	1165			34	15	619	8.00	2226	23.8	2380	2.74	26	7.89	24	636	634	2093	117	2234	1.80	2.81	895	7.86	2303	34.4	2404	2.07	26	7.75	34	918	915	2187	88	2310	1.36	2.12
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	1															2	251			34	15	1171	7.74	2284	45.0	2345	1.57	26	7.63	45	1200	1195	2179	67	2292	1.03	1.61	1711	7.59	2363	65.8	2376	1.15	26	7.48	66	1749	1743	2255	49	2370	0.76	1.18
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	1															2	265			34	15	1171	7.74	2284	45.0	2345	1.57	26	7.63	45	1200	1195	2179	67	2292	1.03	1.61	1711	7.59	2363	65.8	2376	1.15	26	7.48	66	1749	1743	2255	49	2370	0.76	1.18
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	1															4	394			34	15	1171	7.74	2284	45.0	2345	1.57	26	7.63	45	1200	1195	2179	67	2292	1.03	1.61	1711	7.59	2363	65.8	2376	1.15	26	7.48	66	1749	1743	2255	49	2370	0.76	1.18
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	1															4	375			34	15	1171	7.74	2284	45.0	2345	1.57	26	7.63	45	1200	1195	2179	67	2292	1.03	1.61	1711	7.59	2363	65.8	2376	1.15	26	7.48	66	1749	1743	2255	49	2370	0.76	1.18
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	1															7	580			34	15	1171	7.74	2284	45.0	2345	1.57	26	7.63	45	1200	1195	2179	67	2292	1.03	1.61	1711	7.59	2363	65.8	2376	1.15	26	7.48	66	1749	1743	2255	49	2370	0.76	1.18
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	1															8	565			34	15	1171	7.74	2284	45.0	2345	1.57	26	7.63	45	1200	1195	2179	67	2292	1.03	1.61	1711	7.59	2363	65.8	2376	1.15	26	7.48	66	1749	1743	2255	49	2370	0.76	1.18
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	1															8	660			34	15	1171	7.74	2284	45.0	2345	1.57	26	7.63	45	1200	1195	2179	67	2292	1.03	1.61	1711	7.59	2363	65.8	2376	1.15	26	7.48	66	1749	1743	2255	49	2370	0.76	1.18
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	1															8	710			34	15	1171	7.74	2284	45.0	2345	1.57	26	7.63	45	1200	1195	2179	67	2292	1.03	1.61	1711	7.59	2363	65.8	2376	1.15	26	7.48	66	1749	1743	2255	49	2370	0.76	1.18
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	2															2	223			34	15	1224	7.73	2291	47.0	2346	1.51	26	7.61	47	1252	1247	2186	65	2298	1.00	1.56	1721	7.59	2382	66.1	2395	1.16	26	7.48	66	1765	1759	2273	50	2389	0.76	1.19
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	2															2	250			34	15	1224	7.73	2291	47.0	2346	1.51	26	7.61	47	1252	1247	2186	65	2298	1.00	1.56	1721	7.59	2382	66.1	2395	1.16	26	7.48	66	1765	1759	2273	50	2389	0.76	1.19
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	2															4	334			34	15	1224	7.73	2291	47.0	2346	1.51	26	7.61	47	1252	1247	2186	65	2298	1.00	1.56	1721	7.59	2382	66.1	2395	1.16	26	7.48	66	1765	1759	2273	50	2389	0.76	1.19
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	2															4	440			34	15	1224	7.73	2291	47.0	2346	1.51	26	7.61	47	1252	1247	2186	65	2298	1.00	1.56	1721	7.59	2382	66.1	2395	1.16	26	7.48	66	1765	1759	2273	50	2389	0.76	1.19
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	2															7	595			34	15	1224	7.73	2291	47.0	2346	1.51	26	7.61	47	1252	1247	2186	65	2298	1.00	1.56	1721	7.59	2382	66.1	2395	1.16	26	7.48	66	1765	1759	2273	50	2389	0.76	1.19
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	2															8	720			34	15	1224	7.73	2291	47.0	2346	1.51	26	7.61	47	1252	1247	2186	65	2298	1.00	1.56	1721	7.59	2382	66.1	2395	1.16	26	7.48	66	1765	1759	2273	50	2389	0.76	1.19
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	2															8	745			34	15	1224	7.73	2291	47.0	2346	1.51	26	7.61	47	1252	1247	2186	65	2298	1.00	1.56	1721	7.59	2382	66.1	2395	1.16	26	7.48	66	1765	1759	2273	50	2389	0.76	1.19
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	2															8	745			34	15	1224	7.73	2291	47.0	2346	1.51	26	7.61	47	1252	1247	2186	65	2298	1.00	1.56	1721	7.59	2382	66.1	2395	1.16	26	7.48	66	1765	1759	2273	50	2389	0.76	1.19
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	3															2	255			34	15	1214	7.73	2298	46.7	2354	1.54	26	7.61	47	1247	1243	2193	65	2305	1.01	1.57	1727	7.59	2369	66.4	2381	1.15	26	7.48	66	1771	1765	2261	49	2376	0.75	1.18
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	3															2	275			34	15	1214	7.73	2298	46.7	2354	1.54	26	7.61	47	1247	1243	2193	65	2305	1.01	1.57	1727	7.59	2369	66.4	2381	1.15	26	7.48	66	1771	1765	2261	49	2376	0.75	1.18
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	3															4	352			34	15	1214	7.73	2298	46.7	2354	1.54	26	7.61	47	1247	1243	2193	65	2305	1.01	1.57	1727	7.59	2369	66.4	2381	1.15	26	7.48	66	1771	1765	2261	49	2376	0.75	1.18
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	3															4	490			34	15	1214	7.73	2298	46.7	2354	1.54	26	7.61	47	1247	1243	2193	65	2305	1.01	1.57	1727	7.59	2369	66.4	2381	1.15	26	7.48	66	1771	1765	2261	49	2376	0.75	1.18
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	3															7	640			34	15	1214	7.73	2298	46.7	2354	1.54	26	7.61	47	1247	1243	2193	65	2305	1.01	1.57	1727	7.59	2369	66.4	2381	1.15	26	7.48	66	1771	1765	2261	49	2376	0.75	1.18
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	3															8	800			34	15	1214	7.73	2298	46.7	2354	1.54	26	7.61	47	1247	1243	2193	65	2305	1.01	1.57	1727	7.59	2369	66.4	2381	1.15	26	7.48	66	1771	1765	2261	49	2376	0.75	1.18
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	3															8	710			34	15	1214	7.73	2298	46.7	2354	1.54	26	7.61	47	1247	1243	2193	65	2305	1.01	1.57	1727	7.59	2369	66.4	2381	1.15	26	7.48	66	1771	1765	2261	49	2376	0.75	1.18
Figure S1	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm	3															8	645			34	15	1214	7.73	2298	46.7	2354	1.54	26	7.61	47	1247	1243	2193	65	2305	1.01	1.57	1727	7.59	2369	66.4	2381	1.15	26	7.48	66	1771	1765	2261	49	2376	0.75	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Chromatin structure and dynamics	0.8	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Replication, recombination and repair	2.3	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Transcription	2.3	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		RNA processing and modification	4.1	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Translation, ribosomal structure and biogenesis	14.2	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Posttranslational modification, protein turnover and chaperones	14.8	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Intracellular trafficking, secretion and vesicular transport	7.9	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Extracellular structures	0.4	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Cytoskeleton	3.4	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Cell motility	0.0	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Cell wall/membrane/envelope biogenesis	0.8	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Signal transduction mechanisms	10.7	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Defense mechanisms	0.3	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Nuclear structure	0.3	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Cell cycle control, cell division and chromosome partitioning	2.3	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Secondary metabolites biosynthesis, transport and catabolism	2.3	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Inorganic ion transport and metabolism	3.7	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Lipid transport and metabolism	5.1	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Coenzyme transport and metabolism	1.8	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Nucleotide transport and metabolism	2.4	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Amino acid transport and metabolism	4.9	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Carbohydrate transport and metabolism	6.8	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=150 micro atm																		Energy production and conversion	8.7	34	15	151	8.52	1982	5.8	2432	7.21	26	8.40	6	154	154	1677	309	1991	4.75	7.41	243	8.35	2041	9.4	2367	5.24	26	8.23	9	249	249	1816	224	2049	3.45	5.38
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Chromatin structure and dynamics	0.9	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Replication, recombination and repair	1.8	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Transcription	2.7	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		RNA processing and modification	5.4	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Translation, ribosomal structure and biogenesis	13.4	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Posttranslational modification, protein turnover and chaperones	14.0	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Intracellular trafficking, secretion and vesicular transport	8.5	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Extracellular structures	0.3	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Cytoskeleton	3.7	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Cell motility	0.4	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Cell wall/membrane/envelope biogenesis	1.0	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Signal transduction mechanisms	9.5	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Defense mechanisms	0.5	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Nuclear structure	0.3	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Cell cycle control, cell division and chromosome partitioning	2.6	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Secondary metabolites biosynthesis, transport and catabolism	2.0	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Inorganic ion transport and metabolism	3.6	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Lipid transport and metabolism	4.4	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Coenzyme transport and metabolism	2.0	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Nucleotide transport and metabolism	2.7	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Amino acid transport and metabolism	5.0	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Carbohydrate transport and metabolism	6.4	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=380 micro atm																		Energy production and conversion	9.1	34	15	330	8.24	2127	12.7	2395	4.39	26	8.13	13	339	338	1935	188	2135	2.89	4.50	506	8.08	2181	19.4	2367	3.17	26	7.96	19	518	516	2034	136	2189	2.09	3.25
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Chromatin structure and dynamics	0.6	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Replication, recombination and repair	1.9	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Transcription	1.9	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		RNA processing and modification	6.5	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Translation, ribosomal structure and biogenesis	11.9	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Posttranslational modification, protein turnover and chaperones	15.4	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Intracellular trafficking, secretion and vesicular transport	7.8	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Extracellular structures	0.4	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Cytoskeleton	3.7	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Cell motility	0.0	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Cell wall/membrane/envelope biogenesis	0.6	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Signal transduction mechanisms	11.9	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Defense mechanisms	1.1	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Nuclear structure	0.4	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Cell cycle control, cell division and chromosome partitioning	2.6	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Secondary metabolites biosynthesis, transport and catabolism	1.7	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Inorganic ion transport and metabolism	2.2	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Lipid transport and metabolism	3.7	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Coenzyme transport and metabolism	1.5	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Nucleotide transport and metabolism	2.4	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Amino acid transport and metabolism	5.0	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Carbohydrate transport and metabolism	6.3	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18
Figure S3	Thoracosphaera heimii (phytoplankton)	pCO2=1400 micro atm																		Energy production and conversion	10.4	34	15	1203	7.73	2291	46.2	2348	1.54	26	7.62	46	1235	1231	2186	66	2298	1.01	1.58	1719	7.59	2371	66.1	2384	1.15	26	7.48	66	1762	1756	2263	49	2378	0.76	1.18