Not logged in
PANGAEA.
Data Publisher for Earth & Environmental Science

Hüning, Anne K; Melzner, Frank; Thomsen, Jörn; Gutowska, Magdalena A; Krämer, Lars; Frickenhaus, Stephan; Rosenstiel, Philip; Pörtner, Hans-Otto; Philipp, Eva E R; Lucassen, Magnus (2013): Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel: implications for shell formation and energy metabolism, link to supplementary material. PANGAEA, https://doi.org/10.1594/PANGAEA.823198, Supplement to: Hüning, AK et al. (2013): Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel: implications for shell formation and energy metabolism. Marine Biology, 160(8), 1845-1861, https://doi.org/10.1007/s00227-012-1930-9

Always quote citation above when using data! You can download the citation in several formats below.

RIS CitationBibTeX Citation

Abstract:
Marine organisms have to cope with increasing CO2 partial pressures and decreasing pH in the oceans. We elucidated the impacts of an 8-week acclimation period to four seawater pCO2 treatments (39, 113, 243 and 405 Pa/385, 1,120, 2,400 and 4,000 µatm) on mantle gene expression patterns in the blue mussel Mytilus edulis from the Baltic Sea. Based on the M. edulis mantle tissue transcriptome, the expression of several genes involved in metabolism, calcification and stress responses was assessed in the outer (marginal and pallial zone) and the inner mantle tissues (central zone) using quantitative real-time PCR. The expression of genes involved in energy and protein metabolism (F-ATPase, hexokinase and elongation factor alpha) was strongly affected by acclimation to moderately elevated CO2 partial pressures. Expression of a chitinase, potentially important for the calcification process, was strongly depressed (maximum ninefold), correlating with a linear decrease in shell growth observed in the experimental animals. Interestingly, shell matrix protein candidate genes were less affected by CO2 in both tissues. A compensatory process toward enhanced shell protection is indicated by a massive increase in the expression of tyrosinase, a gene involved in periostracum formation (maximum 220-fold). Using correlation matrices and a force-directed layout network graph, we were able to uncover possible underlying regulatory networks and the connections between different pathways, thereby providing a molecular basis of observed changes in animal physiology in response to ocean acidification.
Further details:
Thomsen, Jörn; Melzner, Frank (2010): Seawater carbonate chemistry and biological processes of Mytilus edulis during experiments, 2010. PANGAEA, https://doi.org/10.1594/PANGAEA.756663
Comment:
Mytilus edulis mantle tissue samples taken for this experiment derived from a previous acclimation experiment (Thomsen and Melzner, 2010, doi:10.1007/s00227-010-1527-0). See further details link for data.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
File nameFile nameHüning, Anne K
CommentCommentHüning, Anne K
Uniform resource locator/link to fileURL fileHüning, Anne K
Size:
12 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


File name

Comment

URL file
Huening_etal_2013three data tables with descriptions in xlsx formathdl:10013/epic.42687.d020
227_2012_1930_MOESM1_ESMCorrelation network of genes in a) inner mantle tissue and b) outer mantle tissue based on the R-script after removing pCO2 dependent correlationshdl:10013/epic.42687.d002
227_2012_1930_MOESM2_ESMComparison of Copper-binding site CuA from several tyrosinases and one hemocyaninhdl:10013/epic.42687.d003
227_2012_1930_MOESM3_ESMPhylogenetic tree of protein sequences including CuA sites of several tyrosinases and one hemocyaninhdl:10013/epic.42687.d004