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Nakamura, Masoko; Morita, Masaya; Kurihara, Haruko; Mitarai, Satoshi (2012): Seawater carbonate chemistry and expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera during experiments, 2012 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.779704, Supplement to: Nakamura, M et al. (2012): Expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera under prospective acidified conditions over the next several decades. Biology Open, 1(2), 75-81, https://doi.org/10.1242/bio.2011036

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Abstract:
Ocean acidification is an ongoing threat for marine organisms due to the increasing atmospheric CO2 concentration. Seawater acidification has a serious impact on physiologic processes in marine organisms at all life stages. On the other hand, potential tolerance to external pH changes has been reported in coral larvae. Information about the possible mechanisms underlying such tolerance responses, however, is scarce. In the present study, we examined the effects of acidified seawater on the larvae of Acropora digitifera at the molecular level. We targeted two heat shock proteins, Hsp70 and Hsp90, and a heat shock transcription factor, Hsf1, because of their importance in stress responses and in early life developmental stages. Coral larvae were maintained under the ambient and elevated CO2 conditions that are expected to occur within next 100 years, and then we evaluated the expression of hsps and hsf1 by quantitative real-time polymerase chain reaction (PCR). Expression levels of these molecules significantly differed among target genes, but they did not change significantly between CO2conditions. These findings indicate that the expression of hsps is not changed due to external pH changes, and suggest that tolerance to acidified seawater in coral larvae may not be related to hsp expression.
Keyword(s):
Acropora digitifera; Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Cnidaria; Coast and continental shelf; Gene expression (incl. proteomics); Laboratory experiment; North Pacific; Single species; Temperate
Project(s):
European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)
European Project on Ocean Acidification (EPOCA)
Ocean Acidification International Coordination Centre (OA-ICC)
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Experimental treatmentExp treatNakamura, Masoko
2Experiment dayExp daydayNakamura, Masoko
3SalinitySalNakamura, MasokoSalinometer (AS ONE, IS/Mill-E)
4Temperature, waterTemp°CNakamura, Masoko
5pHpHNakamura, MasokopH meter (Mettler Toledo, USA)NBS scale
6pH, standard errorpH std e±Nakamura, Masoko
7Alkalinity, totalATµmol/kgNakamura, MasokoTotal alkalinity titration analyzer (Kimoto ATT-05, Japan)
8Alkalinity, total, standard errorAT std e±Nakamura, Masoko
9Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNakamura, MasokoCalculated using CO2SYS
10Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard errorpCO2water_SST_wet std e±Nakamura, Masoko
11Bicarbonate ion[HCO3]-µmol/kgNakamura, MasokoCalculated using CO2SYS
12Bicarbonate ion, standard error[HCO3]- std e±Nakamura, Masoko
13Carbonate ion[CO3]2-µmol/kgNakamura, MasokoCalculated using CO2SYS
14Carbonate ion, standard error[CO3]2- std e±Nakamura, Masoko
15Aragonite saturation stateOmega ArgNakamura, MasokoCalculated
16Aragonite saturation state, standard deviationOmega Arg std dev±Nakamura, Masoko
17Acropora digitifera, heat shock protein 70 expressionA. digitifera hsp70#Nakamura, Masokosee reference(s)
18Acropora digitifera, heat shock protein 70 expression, standard errorA. digitifera hsp70 std e±Nakamura, Masoko
19Acropora digitifera, heat shock protein 90 expressionA. digitifera hsp90#Nakamura, Masokosee reference(s)
20Acropora digitifera, heat shock protein 90 expression, standard errorA. digitifera hsp90 std e±Nakamura, Masoko
21Acropora digitifera, heat shock factor 1 expressionA. digitifera hsf1#Nakamura, Masokosee reference(s)
22Acropora digitifera, heat shock factor 1 expression, standard errorA. digitifera hsf1 std e±Nakamura, Masoko
23Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
24pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
25Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
26Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
27Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
28Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
29Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
30Carbon, inorganic, dissolvedDICµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
31Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
32Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
256 data points

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