Not logged in
PANGAEA.
Data Publisher for Earth & Environmental Science

Stumpp, Meike; Trübenbach, Katja; Brennecke, Dennis; Hu, Marian Y; Melzner, Frank (2012): Seawater carbonate chemistry and resource allocation and extracellular acid-base status in the sea urchin Strongylocentrotus droebachiensis during experiments, 2012 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.779697, Supplement to: Stumpp, M et al. (2012): Resource allocation and extracellular acid-base status in the sea urchin Strongylocentrotus droebachiensis in response to CO2 induced seawater acidification. Aquatic Toxicology, 110-111, 194-207, https://doi.org/10.1016/j.aquatox.2011.12.020

Always quote citation above when using data! You can download the citation in several formats below.

RIS CitationBibTeX Citation

Abstract:
Anthropogenic CO2 emission will lead to an increase in seawater pCO2 of up to 80-100 Pa (800-1000 µatm) within this century and to an acidification of the oceans. Green sea urchins (Strongylocentrotus droebachiensis) occurring in Kattegat experience seasonal hypercapnic and hypoxic conditions already today. Thus, anthropogenic CO2 emissions will add up to existing values and will lead to even higher pCO2 values >200 Pa (>2000 µatm). To estimate the green sea urchins' potential to acclimate to acidified seawater, we calculated an energy budget and determined the extracellular acid base status of adult S. droebachiensis exposed to moderately (102 to 145 Pa, 1007 to 1431 µatm) and highly (284 to 385 Pa, 2800 to 3800 µatm) elevated seawater pCO2 for 10 and 45 days.
A 45 - day exposure to elevated pCO2 resulted in a shift in energy budgets, leading to reduced somatic and reproductive growth. Metabolic rates were not significantly affected, but ammonium excretion increased in response to elevated pCO2. This led to decreased O:N ratios. These findings suggest that protein metabolism is possibly enhanced under elevated pCO2 in order to support ion homeostasis by increasing net acid extrusion. The perivisceral coelomic fluid acid-base status revealed that S. droebachiensis is able to fully (intermediate pCO2) or partially (high pCO2) compensate extracellular pH (pHe) changes by accumulation of bicarbonate (maximum increases 2.5 mM), albeit at a slower rate than typically observed in other taxa (10 day duration for full pHe compensation). At intermediate pCO2, sea urchins were able to maintain fully compensated pHe for 45 days. Sea urchins from the higher pCO2 treatment could be divided into two groups following medium-term acclimation: one group of experimental animals (29%) contained remnants of food in their digestive system and maintained partially compensated pHe (+2.3 mM HCO3), while the other group (71%) exhibited an empty digestive system and a severe metabolic acidosis (-0.5 pH units, -2.4 mM HCO3). There was no difference in mortality between the three pCO2 treatments.
The results of this study suggest that S. droebachiensis occurring in the Kattegat might be pre-adapted to hypercapnia due to natural variability in pCO2 in its habitat. We show for the first time that some echinoderm species can actively compensate extracellular pH. Seawater pCO2 values of >200 Pa, which will occur in the Kattegat within this century during seasonal hypoxic events, can possibly only be endured for a short time period of a few weeks. Increases in anthropogenic CO2 emissions and leakages from potential sub-seabed CO2 storage (CCS) sites thus impose a threat to the ecologically and economically important species S. droebachiensis.
Keyword(s):
Animalia; Behaviour; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Echinodermata; Growth/Morphology; Laboratory experiment; North Atlantic; Red Sea; Reproduction; Single species; Strongylocentrotus droebachiensis; Temperate
Project(s):
Biological Impacts of Ocean Acidification (BIOACID)
European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)
European Project on Ocean Acidification (EPOCA)
Ocean Acidification International Coordination Centre (OA-ICC)
Sub-seabed CO2 Storage: Impact on Marine Ecosystems (ECO2)
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Seventh Framework Programme (FP7), grant/award no. 265847: Sub-seabed CO2 Storage: Impact on Marine Ecosystems
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 Time, incubationT incubationdayStumpp, Meike
2 Experimental treatmentExp treatStumpp, Meike
3 Flow rateFlow ratel/minStumpp, Meike
4 Flow rate, standard deviationFlow rate std dev±Stumpp, Meike
5 ReplicatesRepl#Stumpp, MeikeT
6 Temperature, waterTemp°CStumpp, Meike
7 Temperature, standard deviationT std dev±Stumpp, Meike
8 ReplicatesRepl#Stumpp, MeikeS
9 SalinitySalStumpp, Meike
10 Salinity, standard deviationSal std dev±Stumpp, Meike
11 ReplicatesRepl#Stumpp, MeikepH NBS
12 pHpHStumpp, MeikeCalculated using CO2SYSNBS scale
13 pH, standard deviationpH std dev±Stumpp, MeikeNBS scale
14 ReplicatesRepl#Stumpp, MeikepH Total
15 pHpHStumpp, MeikepH meter (Metrohm, 826 pH mobile)Total scale
16 pH, standard deviationpH std dev±Stumpp, MeikeTotal scale
17 ReplicatesRepl#Stumpp, MeikeTA
18 Alkalinity, totalATµmol/kgStumpp, MeikePotentiometric titration, VINDTA (marianda)
19 Alkalinity, total, standard deviationAT std dev±Stumpp, Meike
20 ReplicatesRepl#Stumpp, MeikeTC
21 Carbon, inorganic, dissolvedDICµmol/kgStumpp, Meikesee reference(s)
22 Carbon, inorganic, dissolved, standard deviationDIC std dev±Stumpp, Meike
23 ReplicatesRepl#Stumpp, MeikepCO2
24 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmStumpp, MeikeCalculated using CO2SYS
25 Carbon dioxide, partial pressure, standard deviationpCO2 std dev±Stumpp, Meike
26 ReplicatesRepl#Stumpp, MeikeCalcite
27 Calcite saturation stateOmega CalStumpp, MeikeCalculated using CO2SYS
28 Calcite saturation state, standard deviationOmega Cal std dev±Stumpp, Meike
29 ReplicatesRepl#Stumpp, MeikeAragonite
30 Aragonite saturation stateOmega ArgStumpp, MeikeCalculated using CO2SYS
31 Aragonite saturation state, standard deviationOmega Arg std dev±Stumpp, Meike
32 ReplicatesRepl#Stumpp, MeikeSample
33 Strongylocentrotus droebachiensisS. droebachiensis%Stumpp, Meikeinfections
34 Strongylocentrotus droebachiensisS. droebachiensis%Stumpp, Meikestressed
35 Strongylocentrotus droebachiensisS. droebachiensis%Stumpp, Meikehealthy
36 Strongylocentrotus droebachiensis, feeding stateS. droebachiensis feeding state%Stumpp, MeikeMicroscopyfull digestive system
37 Strongylocentrotus droebachiensis, feeding stateS. droebachiensis feeding state%Stumpp, MeikeMicroscopyfull hind gut
38 Strongylocentrotus droebachiensis, feeding stateS. droebachiensis feeding state%Stumpp, MeikeMicroscopyempty digestive system
39 Gonad stage, developingGon stg dev%Stumpp, MeikeObservedfull
40 Gonad stage, developingGon stg dev%Stumpp, MeikeObservedmedium
41 Gonad stage, developingGon stg dev%Stumpp, MeikeObservedno gonads
42 Strongylocentrotus droebachiensis, coelomic fluid colorS. droebachiensis coelomic fluid%Stumpp, MeikeObservedred
43 Strongylocentrotus droebachiensis, coelomic fluid colorS. droebachiensis coelomic fluid%Stumpp, MeikeObservedlight red
44 Strongylocentrotus droebachiensis, coelomic fluid colorS. droebachiensis coelomic fluid%Stumpp, MeikeObservedcolorless
45 Positioning type/detailsPos typeStumpp, MeikeObservedtank water surface
46 Positioning type/detailsPos typeStumpp, MeikeObservedtank bottom
47 Positioning type/detailsPos typeStumpp, MeikeObservedtank bottom between algae
48 Strongylocentrotus droebachiensis, diameterS. droebachiensis diammmStumpp, MeikeMeasuredstart
49 Strongylocentrotus droebachiensis, diameter, standard deviationS. droebachiensis diam std dev±Stumpp, Meikestart
50 Strongylocentrotus droebachiensis, diameterS. droebachiensis diammmStumpp, MeikeMeasuredend
51 Strongylocentrotus droebachiensis, diameter, standard deviationS. droebachiensis diam std dev±Stumpp, Meikeend
52 Strongylocentrotus droebachiensis, weightS. droebachiensis WgStumpp, MeikePrecision scale (LC220s, Sartorius, Göttingen, Germany, 1 mg resolution)wet mass
53 Strongylocentrotus droebachiensis, weight, standard deviationS. droebachiensis W std dev±Stumpp, Meikewet mass
54 Strongylocentrotus droebachiensis, test, weightS. droebachiensis test WgStumpp, MeikePrecision scale (LC220s, Sartorius, Göttingen, Germany, 1 mg resolution)wet mass
55 Strongylocentrotus droebachiensis, test, weight, standard deviationS. droebachiensis test W std dev±Stumpp, Meikewet mass
56 Strongylocentrotus droebachiensis, lantern of Aristotle, weightS. droebachiensis LA WmgStumpp, MeikePrecision scale (LC220s, Sartorius, Göttingen, Germany, 1 mg resolution)wet mass
57 Strongylocentrotus droebachiensis, lantern of Aristotle, weight, standard deviationS. droebachiensis LA W std dev±Stumpp, Meikewet mass
58 Strongylocentrotus droebachiensis, gonad, weightS. droebachiensis gonad WmgStumpp, MeikePrecision scale (LC220s, Sartorius, Göttingen, Germany, 1 mg resolution)wet mass
59 Strongylocentrotus droebachiensis, gonad, weight, standard deviationS. droebachiensis gonad W std dev±Stumpp, Meikewet mass
60 Strongylocentrotus droebachiensis, gut, weightS. droebachiensis gut WmgStumpp, MeikePrecision scale (LC220s, Sartorius, Göttingen, Germany, 1 mg resolution)wet mass
61 Strongylocentrotus droebachiensis, gut, weight, standard deviationS. droebachiensis gut W std dev±Stumpp, Meikewet mass
62 Strongylocentrotus droebachiensis, weightS. droebachiensis WgStumpp, MeikePrecision scale (LC220s, Sartorius, Göttingen, Germany, 1 mg resolution)dry mass
63 Strongylocentrotus droebachiensis, weight, standard deviationS. droebachiensis W std dev±Stumpp, Meikedry mass
64 Strongylocentrotus droebachiensis, test, weightS. droebachiensis test WgStumpp, MeikePrecision scale (LC220s, Sartorius, Göttingen, Germany, 1 mg resolution)dry mass
65 Strongylocentrotus droebachiensis, test, weight, standard deviationS. droebachiensis test W std dev±Stumpp, Meikedry mass
66 Strongylocentrotus droebachiensis, lantern of Aristotle, weightS. droebachiensis LA WmgStumpp, MeikePrecision scale (LC220s, Sartorius, Göttingen, Germany, 1 mg resolution)dry mass
67 Strongylocentrotus droebachiensis, lantern of Aristotle, weight, standard deviationS. droebachiensis LA W std dev±Stumpp, Meikedry mass
68 Strongylocentrotus droebachiensis, gonad, weightS. droebachiensis gonad WmgStumpp, MeikePrecision scale (LC220s, Sartorius, Göttingen, Germany, 1 mg resolution)dry mass
69 Strongylocentrotus droebachiensis, gonad, weight, standard deviationS. droebachiensis gonad W std dev±Stumpp, Meikedry mass
70 Strongylocentrotus droebachiensis, gut, weightS. droebachiensis gut WmgStumpp, MeikePrecision scale (LC220s, Sartorius, Göttingen, Germany, 1 mg resolution)dry mass
71 Strongylocentrotus droebachiensis, gut, weight, standard deviationS. droebachiensis gut W std dev±Stumpp, Meikedry mass
72 Strongylocentrotus droebachiensis, weightS. droebachiensis WgStumpp, MeikeCalculated, see reference(s)ash-free dry mass
73 Strongylocentrotus droebachiensis, weight, standard deviationS. droebachiensis W std dev±Stumpp, Meikeash-free dry mass
74 Strongylocentrotus droebachiensis, test, weightS. droebachiensis test WgStumpp, MeikeCalculated, see reference(s)ash-free dry mass
75 Strongylocentrotus droebachiensis, test, weight, standard deviationS. droebachiensis test W std dev±Stumpp, Meikeash-free dry mass
76 Strongylocentrotus droebachiensis, lantern of Aristotle, weightS. droebachiensis LA WmgStumpp, MeikeCalculated, see reference(s)ash-free dry mass
77 Strongylocentrotus droebachiensis, lantern of Aristotle, weight, standard deviationS. droebachiensis LA W std dev±Stumpp, Meikeash-free dry mass
78 Strongylocentrotus droebachiensis, gonad, weightS. droebachiensis gonad WmgStumpp, MeikeCalculated, see reference(s)ash-free dry mass
79 Strongylocentrotus droebachiensis, gonad, weight, standard deviationS. droebachiensis gonad W std dev±Stumpp, Meikeash-free dry mass
80 Strongylocentrotus droebachiensis, gut, weightS. droebachiensis gut WmgStumpp, MeikeCalculated, see reference(s)ash-free dry mass
81 Strongylocentrotus droebachiensis, gut, weight, standard deviationS. droebachiensis gut W std dev±Stumpp, Meikeash-free dry mass
82 Strongylocentrotus droebachiensis, weightS. droebachiensis WgStumpp, MeikePrecision scale (LC220s, Sartorius, Göttingen, Germany, 1 mg resolution)ash dry mass
83 Strongylocentrotus droebachiensis, weight, standard deviationS. droebachiensis W std dev±Stumpp, Meikeash dry mass
84 Strongylocentrotus droebachiensis, test, weightS. droebachiensis test WgStumpp, MeikePrecision scale (LC220s, Sartorius, Göttingen, Germany, 1 mg resolution)ash dry mass
85 Strongylocentrotus droebachiensis, test, weight, standard deviationS. droebachiensis test W std dev±Stumpp, Meikeash dry mass
86 Strongylocentrotus droebachiensis, lantern of Aristotle, weightS. droebachiensis LA WmgStumpp, MeikePrecision scale (LC220s, Sartorius, Göttingen, Germany, 1 mg resolution)ash dry mass
87 Strongylocentrotus droebachiensis, lantern of Aristotle, weight, standard deviationS. droebachiensis LA W std dev±Stumpp, Meikeash dry mass
88 Strongylocentrotus droebachiensis, gonad, weightS. droebachiensis gonad WmgStumpp, MeikePrecision scale (LC220s, Sartorius, Göttingen, Germany, 1 mg resolution)ash dry mass
89 Strongylocentrotus droebachiensis, gonad, weight, standard deviationS. droebachiensis gonad W std dev±Stumpp, Meikeash dry mass
90 Strongylocentrotus droebachiensis, gut, weightS. droebachiensis gut WmgStumpp, MeikePrecision scale (LC220s, Sartorius, Göttingen, Germany, 1 mg resolution)ash dry mass
91 Strongylocentrotus droebachiensis, gut, weight, standard deviationS. droebachiensis gut W std dev±Stumpp, Meikeash dry mass
92 Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
93 pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
94 Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
95 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
96 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
97 Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
98 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
99 Alkalinity, totalATµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
100 Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
101 Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
489 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
T incubation [day]		2 
Exp treat		3 
Flow rate [l/min]		4 
Flow rate std dev [±]		5 
Repl [#]
(T)		6 
Temp [°C]		7 
T std dev [±]		8 
Repl [#]
(S)		9 
Sal		10 
Sal std dev [±]		11 
Repl [#]
(pH NBS)		12 
pH
(NBS scale, Calculated using C...)		13 
pH std dev [±]
(NBS scale)		14 
Repl [#]
(pH Total)		15 
pH
(Total scale, pH meter (Metroh...)		16 
pH std dev [±]
(Total scale)		17 
Repl [#]
(TA)		18 
AT [µmol/kg]
(Potentiometric titration, VIN...)		19 
AT std dev [±]		20 
Repl [#]
(TC)		21 
DIC [µmol/kg]
(see reference(s))		22 
DIC std dev [±]		23 
Repl [#]
(pCO2)		24 
pCO2water_SST_wet [µatm]
(Calculated using CO2SYS)		25 
pCO2 std dev [±]		26 
Repl [#]
(Calcite)		27 
Omega Cal
(Calculated using CO2SYS)		28 
Omega Cal std dev [±]		29 
Repl [#]
(Aragonite)		30 
Omega Arg
(Calculated using CO2SYS)		31 
Omega Arg std dev [±]		32 
Repl [#]
(Sample)		33 
S. droebachiensis [%]
(infections)		34 
S. droebachiensis [%]
(stressed)		35 
S. droebachiensis [%]
(healthy)		36 
S. droebachiensis feeding state [%]
(full digestive system, Micros...)		37 
S. droebachiensis feeding state [%]
(full hind gut, Microscopy)		38 
S. droebachiensis feeding state [%]
(empty digestive system, Micro...)		39 
Gon stg dev [%]
(full, Observed)		40 
Gon stg dev [%]
(medium, Observed)		41 
Gon stg dev [%]
(no gonads, Observed)		42 
S. droebachiensis coelomic fluid [%]
(red, Observed)		43 
S. droebachiensis coelomic fluid [%]
(light red, Observed)		44 
S. droebachiensis coelomic fluid [%]
(colorless, Observed)		45 
Pos type
(tank water surface, Observed)		46 
Pos type
(tank bottom, Observed)		47 
Pos type
(tank bottom between algae, Ob...)		48 
S. droebachiensis diam [mm]
(start, Measured)		49 
S. droebachiensis diam std dev [±]
(start)		50 
S. droebachiensis diam [mm]
(end, Measured)		51 
S. droebachiensis diam std dev [±]
(end)		52 
S. droebachiensis W [g]
(wet mass, Precision scale (LC...)		53 
S. droebachiensis W std dev [±]
(wet mass)		54 
S. droebachiensis test W [g]
(wet mass, Precision scale (LC...)		55 
S. droebachiensis test W std dev [±]
(wet mass)		56 
S. droebachiensis LA W [mg]
(wet mass, Precision scale (LC...)		57 
S. droebachiensis LA W std dev [±]
(wet mass)		58 
S. droebachiensis gonad W [mg]
(wet mass, Precision scale (LC...)		59 
S. droebachiensis gonad W std dev [±]
(wet mass)		60 
S. droebachiensis gut W [mg]
(wet mass, Precision scale (LC...)		61 
S. droebachiensis gut W std dev [±]
(wet mass)		62 
S. droebachiensis W [g]
(dry mass, Precision scale (LC...)		63 
S. droebachiensis W std dev [±]
(dry mass)		64 
S. droebachiensis test W [g]
(dry mass, Precision scale (LC...)		65 
S. droebachiensis test W std dev [±]
(dry mass)		66 
S. droebachiensis LA W [mg]
(dry mass, Precision scale (LC...)		67 
S. droebachiensis LA W std dev [±]
(dry mass)		68 
S. droebachiensis gonad W [mg]
(dry mass, Precision scale (LC...)		69 
S. droebachiensis gonad W std dev [±]
(dry mass)		70 
S. droebachiensis gut W [mg]
(dry mass, Precision scale (LC...)		71 
S. droebachiensis gut W std dev [±]
(dry mass)		72 
S. droebachiensis W [g]
(ash-free dry mass, Calculated...)		73 
S. droebachiensis W std dev [±]
(ash-free dry mass)		74 
S. droebachiensis test W [g]
(ash-free dry mass, Calculated...)		75 
S. droebachiensis test W std dev [±]
(ash-free dry mass)		76 
S. droebachiensis LA W [mg]
(ash-free dry mass, Calculated...)		77 
S. droebachiensis LA W std dev [±]
(ash-free dry mass)		78 
S. droebachiensis gonad W [mg]
(ash-free dry mass, Calculated...)		79 
S. droebachiensis gonad W std dev [±]
(ash-free dry mass)		80 
S. droebachiensis gut W [mg]
(ash-free dry mass, Calculated...)		81 
S. droebachiensis gut W std dev [±]
(ash-free dry mass)		82 
S. droebachiensis W [g]
(ash dry mass, Precision scale...)		83 
S. droebachiensis W std dev [±]
(ash dry mass)		84 
S. droebachiensis test W [g]
(ash dry mass, Precision scale...)		85 
S. droebachiensis test W std dev [±]
(ash dry mass)		86 
S. droebachiensis LA W [mg]
(ash dry mass, Precision scale...)		87 
S. droebachiensis LA W std dev [±]
(ash dry mass)		88 
S. droebachiensis gonad W [mg]
(ash dry mass, Precision scale...)		89 
S. droebachiensis gonad W std dev [±]
(ash dry mass)		90 
S. droebachiensis gut W [mg]
(ash dry mass, Precision scale...)		91 
S. droebachiensis gut W std dev [±]
(ash dry mass)		92 
CSC flag
(Calculated using seacarb afte...)		93 
pH
(Total scale, Calculated using...)		94 
CO2 [µmol/kg]
(Calculated using seacarb afte...)		95 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		96 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		97 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)		98 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)		99 
AT [µmol/kg]
(Calculated using seacarb afte...)		100 
Omega Arg
(Calculated using seacarb afte...)		101 
Omega Cal
(Calculated using seacarb afte...)
10control pCO20.02800.0008410.50.6431.30.248.020.0247.800.0920246531950903691931.610.2531.020.16332.70.6277.9122.82519.62517.661841.8585.332056.601.312.08
10intermediate pCO20.02800.0008410.30.3431.40.147.550.0147.510.10209246320981731454130.900.2530.570.16332.20.7277.4473.151655.491649.231993.6931.152072.100.480.76
10high pCO20.02800.0008410.40.2431.30.147.130.0347.100.03212446322686533854130.360.0230.230.01332.60.3277.02198.414502.434485.412057.3612.232088.000.190.30
45control pCO20.02760.0008610.40.2629.50.068.000.0367.980.0442147124201617446542.450.1841.550.11400100722806139061390559027.90.229.30.55.560.153.760.105612662774613582.380.071.800.05299121432213810527.0036.00225.0010.0048513222123101.850.041.580.0425111102162277.8925.50572.76570.591910.6079.902109.101.231.96
45intermediate pCO20.02770.0008610.40.2629.60.067.660.0167.670.01421561042109214102441.260.0340.790.02460946733039610673308207127.80.228.90.55.170.263.560.195534350762557372.260.111.720.0829625112161327483.0034.00215.0014.004691041511881.780.081.510.062501982143277.5558.681318.631313.642011.7638.562107.700.590.95
45high pCO20.02770.0009610.40.2629.50.067.190.0267.250.024219510422861642841240.510.0240.320.01400100505000100033067810528.00.428.00.34.700.433.270.295184142982505582.070.131.590.1127519901512112422.0035.00193.0015.004358213108111.650.101.390.092321583122277.08180.344050.214034.902092.1113.552125.700.210.33
45high pCO271380001000802003367
reference group (pre-incubation)28.00.64.070.333.170.284514217642281122.060.181.670.16281373914766369.0072.00198.0010.0037737136941.720.171.470.17234202172