Citation:

ab00266

Name: Seawater carbonate chemistry and crab Necora puber size and elements in tissue during experiments, 2010
Published: 2010
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Acid-base regulation; Animalia; Arthropoda; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Necora puber; North Atlantic; Respiration; Single species; Temperate

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Abstract:

Ocean acidification (OA) is predicted to play a major role in shaping species biogeography and marine biodiversity over the next century. We tested the effect of medium-term exposure to OA (pH 8.00, 7.30 and 6.70 for 30 d) on acid-base balance in the decapod crab Necora puber-a species that is known to possess good extracellular buffering ability during short-term exposure to hypercapnic conditions. To determine if crabs undergo physiological trade-offs in order to buffer their haemolymph, we characterised a number of fundamental physiological functions, i.e. metabolic rate, tolerance to heat, carapace and chelae [Ca2+] and [Mg2+], haemolymph [Ca2+] and [Mg2+], and immune response in terms of lipid peroxidation. Necora puber was able to buffer changes to extracellular pH over 30 d exposure to hypercapnic water, with no evidence of net shell dissolution, thus demonstrating that HCO3- is actively taken up from the surrounding water. In addition, tolerance to heat, carapace mineralization, and aspects of immune response were not affected by hypercapnic conditions. In contrast, whole-animal O2uptake significantly decreased with hypercapnia, while significant increases in haemolymph [Ca2+] and [Mg2+] and chelae [Mg2+] were observed with hypercapnia. Our results confirm that most physiological functions in N. puber are resistant to low pH/hypercapnia over a longer period than previously investigated, although such resistance comes at the expenses of metabolic rates, haemolymph chemistry and chelae mineralization.

Keyword(s):

Acid-base regulation; Animalia; Arthropoda; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Necora puber; North Atlantic; Respiration; Single species; Temperate

Project(s):

European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)

European Project on Ocean Acidification (EPOCA)

Ocean Acidification International Coordination Centre (OA-ICC)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Sixth Framework Programme (FP6), grant/award no. 511106 : European network of excellence for Ocean Ecosystems Analysis

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Experimental treatment	Exp treat		Small, Daniel		pH
2	Experimental treatment	Exp treat		Small, Daniel		Temp
3	Identification	ID		Small, Daniel
4	Sex	Sex		Small, Daniel
5	Necora puber, mass	N. puber m	g	Small, Daniel		Start
6	Necora puber, width	N. puber w	mm	Small, Daniel
7	Comment	Comment		Small, Daniel
8	Necora puber, mass	N. puber m	g	Small, Daniel		End
9	Oxygen consumption	O2 con	ml/g/min	Small, Daniel	Strathkelvin oxygen electrode system
10	Thermal limit	T lim	°C	Small, Daniel	see reference(s)
11	Thermal limit	T lim	°C	Small, Daniel	see reference(s)
12	Necora puber, haemolymph, total carbon	N. puber TC (ha)	mmol/l	Small, Daniel	Automated CO2 analyzer (CIBA-Corning 965, UK)
13	Necora puber, haemolymph, pH	N. puber pH (ha)		Small, Daniel	pH meter (Mettler Toledo, USA)
14	Necora puber, haemolymph, partial pressure of carbon dioxide	N. puber pCO2 (ha)	kPa	Small, Daniel	Calculated, see reference(s)
15	Necora puber, haemolymph, lipid peroxide	N. puber LPO (ha)	nmol/l	Small, Daniel
16	Necora puber, haemolymph, magnesium ion	N. puber Mg2+ (ha)	mmol/l	Small, Daniel	Atomic absorption spectrometer (Spectr AA 600, Varian)
17	Necora puber, haemolymph, calcium ion	N. puber Ca2+ (ha)	mmol/l	Small, Daniel	Atomic absorption spectrometer (Spectr AA 600, Varian)
18	Necora puber, chelae, calcium	N. puber Ca chelae	mmol/g	Small, Daniel	Atomic absorption spectrometer (Spectr AA 600, Varian)
19	Necora puber, chelae, magnesium	N. puber Mg chelae	mmol/g	Small, Daniel	Atomic absorption spectrometer (Spectr AA 600, Varian)
20	Necora puber, carapace, calcium	N. puber Ca carapace	mmol/g	Small, Daniel	Atomic absorption spectrometer (Spectr AA 600, Varian)
21	Necora puber, carapace, magnesium	N. puber Mg carapace	mmol/g	Small, Daniel	Atomic absorption spectrometer (Spectr AA 600, Varian)
22	Salinity	Sal		Small, Daniel	Conductivity meter (WTW, Weilheim, Gemany)
23	Salinity, standard deviation	Sal std dev	±	Small, Daniel
24	Temperature, water	Temp	°C	Small, Daniel	Conductivity meter (WTW, Weilheim, Gemany)
25	Temperature, standard deviation	T std dev	±	Small, Daniel
26	pH	pH		Small, Daniel	pH meter (Mettler Toledo, USA)	NBS scale
27	pH, standard deviation	pH std dev	±	Small, Daniel
28	Carbon, inorganic, dissolved	DIC	µmol/kg	Small, Daniel	Automated CO2 analyzer (CIBA-Corning 965, UK)
29	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Small, Daniel
30	Alkalinity, total	AT	µmol/kg	Small, Daniel	Calculated using CO2SYS
31	Alkalinity, total, standard deviation	AT std dev	±	Small, Daniel
32	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Small, Daniel	Calculated using CO2SYS
33	Carbon dioxide, partial pressure, standard deviation	pCO2 std dev	±	Small, Daniel
34	Calcite saturation state	Omega Cal		Small, Daniel	Calculated using CO2SYS
35	Calcite saturation state, standard deviation	Omega Cal std dev	±	Small, Daniel
36	Aragonite saturation state	Omega Arg		Small, Daniel	Calculated using CO2SYS
37	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Small, Daniel
38	Bicarbonate ion	[HCO3]-	µmol/kg	Small, Daniel	Calculated using CO2SYS
39	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Small, Daniel
40	Carbonate ion	[CO3]2-	µmol/kg	Small, Daniel	Calculated using CO2SYS
41	Carbonate ion, standard deviation	[CO3]2- std dev	±	Small, Daniel
42	Carbonate system computation flag	CSC flag		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
43	pH	pH		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Total scale
44	Carbon dioxide	CO2	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
45	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
46	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
47	Bicarbonate ion	[HCO3]-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
48	Carbonate ion	[CO3]2-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
49	Alkalinity, total	AT	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
50	Aragonite saturation state	Omega Arg		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
51	Calcite saturation state	Omega Cal		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1687 data points

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