Langer, Gerald; Oetjen, Kerstin; Brenneis, Tina (2012): Seawater carbonate chemistry, nutrients, and Calcidiscus leptoporus (strain RCC1135) growth rate and calcification rate during experiments, 2012 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.778475, Supplement to: Langer, G et al. (2012): Calcification of Calcidiscus leptoporus under nitrogen and phosphorus limitation. Journal of Experimental Marine Biology and Ecology, 413, 131-137, https://doi.org/10.1016/j.jembe.2011.11.028
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Abstract:
The coccolithophore Calcidiscus leptoporus was grown in batch culture under nitrogen (N) as well as phosphorus (P) limitation. Growth rate, particulate inorganic carbon (PIC), particulate organic carbon (POC), particulate organic nitrogen (PON), and particulate organic phosphorus (POP) production were determined and coccolith morphology was analysed. While PON production decreased by 70% under N-limitation and POP production decreased by 65% under P-limitation, growth rate decreased by 33% under N- as well as P-limitation. POC as well as PIC production (calcification rate) increased by 27% relative to the control under P-limitation, and did not change under N-limitation. Coccolith morphology did not change in response to either P or N limitation. While these findings, supported by a literature survey, suggest that coccolith morphogenesis is not hampered by either P or N limitation, calcification rate might be. The latter conclusion is in apparent contradiction to our data. We discuss the reasons for this inference.
Keyword(s):
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcidiscus leptoporus; Calcification/Dissolution; Chromista; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Macro-nutrients; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; South Atlantic
Project(s):
Funding:
Seventh Framework Programme (FP7), grant/award no. 265103: Mediterranean Sea Acidification in a Changing Climate
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
238 data points
Data
1 Exp treat | 2 Sample ID | 3 Species | 4 Sal (Tetra Con 325 salinity and te...) | 5 Temp [°C] | 6 L:D [hh:hh] (Measured) | 7 PAR [µmol/m2/s] | 8 AT [µmol/kg] (Alkalinity, Gran titration (G...) | 9 AT std dev [±] | 10 pH (NBS scale, pH meter, WTW, 340i) | 11 pH std dev [±] | 12 DIC [µmol/kg] (Seal QuAAtro SFA Analyzer, Se...) | 13 DIC std dev [±] | 14 [PO4]3- [µmol/kg] (Autoanalyzer) | 15 [PO4]3- std dev [±] | 16 [NO3]- [mmol/kg] (Autoanalyzer) | 17 NO3 std dev [±] | 18 C. leptoporus [%] (Normal) | 19 C. leptoporus std dev [±] (Normal) | 20 C. leptoporus [%] (Malformed) | 21 C. leptoporus std dev [±] (Malformed) | 22 C. leptoporus [%] (Incomplete) | 23 C. leptoporus std dev [±] (Incomplete) | 24 C. leptoporus [%] (Incompete and malformed) | 25 C. leptoporus std dev [±] (Incompete and malformed) | 26 µ [#/day] | 27 µ std dev [±] | 28 POC/cell [pg/#] (Element analyser CNS, EURO EA) | 29 POC cont/cell std dev [±] | 30 POC prod/cell [pg/#/day] (Calculated, see reference(s)) | 31 POC prod std dev [±] | 32 PIC/cell [pg/#] (Calculated, see reference(s)) | 33 PIC/cell std dev [±] | 34 Calc rate C/cell [pg/#/h] | 35 Calc rate std dev [±] | 36 POP/cell [pmol/#] (Original unit pg/cell, value ...) | 37 POP/cell std dev [±] | 38 POP prod/cell [pmol/#/day] (Original unit pg/cell, value ...) | 39 POP prod [µmol/l/day] (Original unit pg/cell, value ...) | 40 PON/cell [pg/#] (Element analyser CNS, EURO EA) | 41 PON/cell std dev [±] | 42 PON prod [pg/#/day] (Calculated, see reference(s)) | 43 PON prod std dev [±] | 44 CSC flag (Calculated using seacarb afte...) | 45 pH (total scale, Calculated using...) | 46 CO2 [µmol/kg] (Calculated using seacarb afte...) | 47 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 48 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 49 [HCO3]- [µmol/kg] (Calculated using seacarb afte...) | 50 [CO3]2- [µmol/kg] (Calculated using seacarb afte...) | 51 DIC [µmol/kg] (Calculated using seacarb afte...) | 52 Omega Arg (Calculated using seacarb afte...) | 53 Omega Cal (Calculated using seacarb afte...) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Control | T0 | Calcidiscus leptoporus (strain RCC1135) | 32 | 15 | 16:08 | 400 | 2462 | 3 | 7.94 | 0.004 | 2210 | 7 | 32.96 | 0.29 | 0.7274 | 0.0053 | 26 | 7.82 | 29.88 | 787.18 | 784.37 | 2215.64 | 101.68 | 2347.2 | 1.58 | 2.47 | ||||||||||||||||||||||||||
Control | Tfin | Calcidiscus leptoporus (strain RCC1135) | 32 | 15 | 16:08 | 400 | 2208 | 19 | 7.87 | 0.009 | 2015 | 18 | 31.45 | 0.15 | 0.6897 | 0.0029 | 43.83 | 2.75 | 20.66 | 3.24 | 2.88 | 1.42 | 32.63 | 5.27 | 0.54 | 0.02 | 30.53 | 1.79 | 16.53 | 0.57 | 70.81 | 4.50 | 1.597083333 | 0.068 | 0.01 | 0 | 0.007 | 0.001 | 10.45 | 1.38 | 5.65 | 0.62 | 26 | 7.75 | 31.91 | 840.64 | 837.64 | 2013.56 | 78.64 | 2124.1 | 1.22 | 1.91 |
PO4limited | T0 | Calcidiscus leptoporus (strain RCC1135) | 32 | 15 | 16:08 | 400 | 2429 | 2 | 7.98 | 0.012 | 2194 | 4 | 0.25 | 0.01 | 0.7267 | 0.0034 | 26 | 7.86 | 26.62 | 701.21 | 698.71 | 2164.45 | 108.93 | 2300.0 | 1.69 | 2.65 | ||||||||||||||||||||||||||
PO4limited | Tfin | Calcidiscus leptoporus (strain RCC1135) | 32 | 15 | 16:08 | 400 | 1956 | 40 | 7.79 | 0.019 | 1835 | 23 | 0.00 | 0.00 | 0.7132 | 0.0057 | 47.20 | 1.34 | 26.59 | 0.94 | 1.90 | 0.55 | 24.41 | 0.87 | 0.36 | 0.01 | 61.82 | 8.51 | 22.63 | 2.46 | 144.71 | 12.39 | 2.179583333 | 0.160 | 0.01 | 0 | 0.002 | 0.001 | 12.97 | 1.35 | 4.75 | 0.44 | 26 | 7.67 | 34.42 | 906.82 | 903.59 | 1807.16 | 58.72 | 1900.3 | 0.91 | 1.43 |
NO3limited | T0 | Calcidiscus leptoporus (strain RCC1135) | 32 | 15 | 16:08 | 400 | 2462 | 3 | 7.98 | 0.008 | 2190 | 8 | 34.98 | 0.25 | 0.0060 | 0.0001 | 26 | 7.86 | 26.97 | 710.64 | 708.11 | 2194.35 | 110.48 | 2331.8 | 1.71 | 2.68 | ||||||||||||||||||||||||||
NO3limited | Tfin | Calcidiscus leptoporus (strain RCC1135) | 32 | 15 | 16:08 | 400 | 1896 | 42 | 7.71 | 0.026 | 34.42 | 0.23 | 0.0001 | 0.0001 | 48.25 | 1.40 | 20.55 | 3.31 | 1.25 | 0.42 | 29.95 | 4.24 | 0.36 | 0.01 | 42.37 | 3.16 | 15.24 | 0.95 | 107.13 | 5.26 | 1.597916667 | 0.084 | 0.02 | 0 | 0.006 | 0.001 | 4.79 | 1.00 | 1.72 | 0.32 | 26 | 7.59 | 40.64 | 1070.78 | 1066.97 | 1773.75 | 47.91 | 1862.3 | 0.74 | 1.16 |