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Munday, Philip L; Hernaman, V; Dixson, Danielle L; Thorrold, Simon R (2011): Seawater carbonate chemistry and clownfish Amphiprion percula size and otholith development during experiments, 2011 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.778199, Supplement to: Munday, PL et al. (2011): Effect of ocean acidification on otolith development in larvae of a tropical marine fish. Biogeosciences, 8(6), 1631-1641, https://doi.org/10.5194/bg-8-1631-2011

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Abstract:
Calcification in many invertebrate species is predicted to decline due to ocean acidification. The potential effects of elevated CO2 and reduced carbonate saturation state on other species, such as fish, are less well understood. Fish otoliths (earbones) are composed of aragonite, and thus, might be susceptible to either the reduced availability of carbonate ions in seawater at low pH, or to changes in extracellular concentrations of bicarbonate and carbonate ions caused by acid-base regulation in fish exposed to high pCO2. We reared larvae of the clownfish Amphiprion percula from hatching to settlement at three pHNBS and pCO2 levels (control: ~pH 8.15 and 404 µatm CO2; intermediate: pH 7.8 and 1050 µatm CO2; extreme: pH 7.6 and 1721 µatm CO2) to test the possible effects of ocean acidification on otolith development. There was no effect of the intermediate treatment (pH 7.8 and 1050 µatm CO2) on otolith size, shape, symmetry between left and right otoliths, or otolith elemental chemistry, compared with controls. However, in the more extreme treatment (pH 7.6 and 1721 µatm CO2) otolith area and maximum length were larger than controls, although no other traits were significantly affected. Our results support the hypothesis that pH regulation in the otolith endolymph can lead to increased precipitation of CaCO3 in otoliths of larval fish exposed to elevated CO2, as proposed by an earlier study, however, our results also show that sensitivity varies considerably among species. Importantly, our results suggest that otolith development in clownfishes is robust to even the more pessimistic changes in ocean chemistry predicted to occur by 2100.
Keyword(s):
Amphiprion percula; Animalia; Biomass/Abundance/Elemental composition; Calcification/Dissolution; Chordata; Containers and aquaria (20-1000 L or < 1 m**2); Growth/Morphology; Laboratory experiment; Laboratory strains; Nekton; Pelagos; Single species; South Pacific
Project(s):
European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)
European Project on Ocean Acidification (EPOCA)
Ocean Acidification International Coordination Centre (OA-ICC)
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 SpeciesSpeciesMunday, Philip L
2 CommentCommentMunday, Philip L
3 Otolith, Lithium/Calcium ratioOtolith Li/Caµmol/molMunday, Philip LLA-ICP-MS Thermo Finnigan Element 2
4 Otolith, Lithium/Calcium ratio, standard errorOtolith Li/Ca std e±Munday, Philip L
5 Otolith, Magnesium/Calcium ratioOtolith Mg/Caµmol/molMunday, Philip LLA-ICP-MS Thermo Finnigan Element 2
6 Otolith, Magnesium/Calcium ratio, standard errorOtolith Mg/Ca std e±Munday, Philip L
7 Otolith, Manganese/Calcium ratioOtolith Mn/Caµmol/molMunday, Philip LLA-ICP-MS Thermo Finnigan Element 2
8 Otolith, Manganese/Calcium ratio, standard errorOtolith Mn/Ca std e±Munday, Philip L
9 Otolith, Strontium/Calcium ratioOtolith Sr/Cammol/molMunday, Philip LLA-ICP-MS Thermo Finnigan Element 2
10 Otolith, Strontium/Calcium ratio, standard errorOtolith Sr/Ca std e±Munday, Philip L
11 Otolith, Barium/Calcium ratioOtolith Ba/Caµmol/molMunday, Philip LLA-ICP-MS Thermo Finnigan Element 2
12 Otolith, Barium/Calcium ratio, standard errorOtolith Ba/Ca std e±Munday, Philip L
13 Amphiprion percula, otolith, areaA. percula otolith areaµm2Munday, Philip Lsee reference(s)
14 Amphiprion percula, otolith, area, standard errorA. percula otolith area std e±Munday, Philip L
15 Amphiprion percula, otolith, lengthA. percula otolith lµmMunday, Philip Lsee reference(s)
16 Amphiprion percula, otolith, length, standard errorA. percula otolith l std e±Munday, Philip L
17 Amphiprion percula, otolith, widthA. percula otolith wµmMunday, Philip Lsee reference(s)
18 Amphiprion percula, otolith, width standard errorA. percula otolith w std e±Munday, Philip L
19 Amphiprion percula, otolith, rectangularityA. percula otolith rMunday, Philip Lsee reference(s)
20 Amphiprion percula, otolith, rectangularity, standard errorA. percula otolith r std e±Munday, Philip L
21 Amphiprion percula, otolith, circularityA. percula otolith cMunday, Philip Lsee reference(s)
22 Amphiprion percula, otolith, circularity, standard errorA. percula otolith c std e±Munday, Philip L
23 Amphiprion percula, lengthA. percula otolith lµmMunday, Philip LImage analysis
24 Amphiprion percula, length, standard errorA. percula otolith l std e±Munday, Philip L
25 pHpHMunday, Philip LpH meter (TPS WP80)NBS scale; mean
26 pHpHMunday, Philip LpH meter (TPS WP80)NBS scale; min
27 pHpHMunday, Philip LpH meter (TPS WP80)NBS scale; max
28 SalinitySalMunday, Philip L
29 Temperature, waterTemp°CMunday, Philip L
30 Temperature, standard deviationT std dev±Munday, Philip LTitration
31 Alkalinity, totalATµmol/kgMunday, Philip L
32 Alkalinity, total, standard deviationAT std dev±Munday, Philip L
33 Carbon, inorganic, dissolvedDICµmol/kgMunday, Philip LCalculated using CO2SYS
34 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmMunday, Philip LCalculated using CO2SYS
35 Carbon dioxideCO2µmol/kgMunday, Philip LCalculated using CO2SYS
36 Bicarbonate ion[HCO3]-µmol/kgMunday, Philip LCalculated using CO2SYS
37 Carbonate ion[CO3]2-µmol/kgMunday, Philip LCalculated using CO2SYS
38 Aragonite saturation stateOmega ArgMunday, Philip LCalculated using CO2SYS
39 Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
40 pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
41 Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
42 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
43 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
44 Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
45 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
46 Carbon, inorganic, dissolvedDICµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
47 Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
48 Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
354 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Species		2 
Comment		3 
Otolith Li/Ca [µmol/mol]
(LA-ICP-MS Thermo Finnigan Ele...)		4 
Otolith Li/Ca std e [±]		5 
Otolith Mg/Ca [µmol/mol]
(LA-ICP-MS Thermo Finnigan Ele...)		6 
Otolith Mg/Ca std e [±]		7 
Otolith Mn/Ca [µmol/mol]
(LA-ICP-MS Thermo Finnigan Ele...)		8 
Otolith Mn/Ca std e [±]		9 
Otolith Sr/Ca [mmol/mol]
(LA-ICP-MS Thermo Finnigan Ele...)		10 
Otolith Sr/Ca std e [±]		11 
Otolith Ba/Ca [µmol/mol]
(LA-ICP-MS Thermo Finnigan Ele...)		12 
Otolith Ba/Ca std e [±]		13 
A. percula otolith area [µm2]
(see reference(s))		14 
A. percula otolith area std e [±]		15 
A. percula otolith l [µm]
(see reference(s))		16 
A. percula otolith l std e [±]		17 
A. percula otolith w [µm]
(see reference(s))		18 
A. percula otolith w std e [±]		19 
A. percula otolith r
(see reference(s))		20 
A. percula otolith r std e [±]		21 
A. percula otolith c
(see reference(s))		22 
A. percula otolith c std e [±]		23 
A. percula otolith l [µm]
(Image analysis)		24 
A. percula otolith l std e [±]		25 
pH
(NBS scale; mean, pH meter (TP...)		26 
pH
(NBS scale; min, pH meter (TPS...)		27 
pH
(NBS scale; max, pH meter (TPS...)		28 
Sal		29 
Temp [°C]		30 
T std dev [±]
(Titration)		31 
AT [µmol/kg]		32 
AT std dev [±]		33 
DIC [µmol/kg]
(Calculated using CO2SYS)		34 
pCO2water_SST_wet [µatm]
(Calculated using CO2SYS)		35 
CO2 [µmol/kg]
(Calculated using CO2SYS)		36 
[HCO3]- [µmol/kg]
(Calculated using CO2SYS)		37 
[CO3]2- [µmol/kg]
(Calculated using CO2SYS)		38 
Omega Arg
(Calculated using CO2SYS)		39 
CSC flag
(Calculated using seacarb afte...)		40 
pH
(Total scale, Calculated using...)		41 
CO2 [µmol/kg]
(Calculated using seacarb afte...)		42 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		43 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		44 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)		45 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)		46 
DIC [µmol/kg]
(Calculated using seacarb afte...)		47 
Omega Arg
(Calculated using seacarb afte...)		48 
Omega Cal
(Calculated using seacarb afte...)
Amphiprion perculaLeft otoliths0.04100.0029273.679110.1001196.67218.81170.75010.004617.34300.82657.21010.43848.158.108.2033.00030120431311768404.110.241567.22190.453.14268.0010.21402.96401.771562.36192.931765.503.184.77
Amphiprion perculaLeft otoliths0.03890.0039261.106714.1717194.799310.24580.73680.006817.71620.57708.02050.30557.807.757.8533.000301206611619461050.826.641820.6898.831.63267.6526.601049.361046.251817.81100.291944.701.652.48
Amphiprion perculaLeft otoliths0.04890.0022302.84076.4024214.34687.20000.73320.017715.90680.23136.89620.30517.607.557.6533.000301204213219901721.443.631881.8565.011.06267.4543.581719.121714.031879.8665.471988.901.081.62
Amphiprion perculaRight ototliths0.04300.0034269.764313.1348204.28688.80760.75820.004416.74270.54947.21010.43848.158.108.2033.00030120431311768404.110.241567.22190.453.14268.0010.21402.96401.771562.36192.931765.503.184.77
Amphiprion perculaRight ototliths0.03900.0042258.534715.4025196.946110.52920.73090.007217.55020.67518.02050.30557.807.757.8533.000301206611619461050.826.641820.6898.831.63267.6526.601049.361046.251817.81100.291944.701.652.48
Amphiprion perculaRight ototliths0.04780.0019298.23484.9206213.31256.41110.74840.004515.66010.18316.89620.30517.607.557.6533.000301204213219901721.443.631881.8565.011.06267.4543.581719.121714.031879.8665.471988.901.081.62
Amphiprion perculaUnsigned differences0.0000050420.00000045560.692814.73780.73450.16593.05560.09715.97531.43000.00780.002827.129311.473123.99636.81758.158.108.2033.00030120431311768404.110.241567.22190.453.14268.0010.21402.96401.771562.36192.931765.503.184.77
Amphiprion perculaUnsigned differences0.0000051140.00000029779.215723.91100.71790.22852.92720.08024.01890.15560.00640.002519.94119.513419.25446.91737.807.757.8533.000301206611619461050.826.641820.6898.831.63267.6526.601049.361046.251817.81100.291944.701.652.48
Amphiprion perculaUnsigned differences0.0000049910.00000044074.541724.74520.87240.20103.03400.20655.40091.69600.00260.00067.56611.493322.487112.97967.607.557.6533.000301204213219901721.443.631881.8565.011.06267.4543.581719.121714.031879.8665.471988.901.081.62