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Rokitta, Sebastian D; Rost, Björn (2012): Seawater carbonate chemistry and effects of CO2 and their modulation by light in the life-cycle stages of the coccolithophore Emiliania huxleyi strains RCC 1216 and 1217 during experiments, 2012 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.777432, Supplement to: Rokitta, SD; Rost, B (2012): Effects of CO2 and their modulation by light in the life-cycle stages of the coccolithophore Emiliania huxleyi. Limnology and Oceanography, 57(2), 607-618, https://doi.org/10.4319/lo.2012.57.2.0607

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Abstract:
The effects of ocean acidification on the life-cycle stages of the coccolithophore Emiliania huxleyi and their by light were examined. Calcifying diploid and noncalcifying haploid cells (Roscoff culture collection 1216 and 1217) were acclimated to present-day and elevated CO2 partial pressures (PCO2; 38.5 vs. 101.3 Pa, ., 380 vs. 1000 matm) under low and high light (50 vs. 300 mmol photons m-2 s-1). Growth rates as well as quotas and production rates of C and N were measured. Sources of inorganic C for biomass buildup were using a 14C disequilibrium assay. Photosynthetic O2 evolution was measured as a function of dissolved inorganic C and light by means of membrane-inlet mass spectrometry. The diploid stage responded to elevated PCO2 by shunting resources from the production of particulate inorganic C toward organic C yet keeping the production of total particulate C constant. As the effect of ocean acidification was stronger under low light, the diploid stage might be less affected by increased acidity when energy availability is high. The haploid stage maintained elemental composition and production rates under elevated PCO2. Although both life-cycle stages involve different ways of dealing with elevated PCO2, the responses were generally modulated by energy availability, being typically most pronounced under low light. Additionally, PCO2 responses resembled those induced by high irradiances, indicating that ocean acidification affects the interplay between energy-generating processes (photosynthetic light reactions) and processes competing for energy (biomass buildup and calcification). A conceptual model is put forward explaining why the magnitude of single responses is determined by energy availability.
Keyword(s):
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Light; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species
Project(s):
European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)
European Project on Ocean Acidification (EPOCA)
Ocean Acidification International Coordination Centre (OA-ICC)
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 SpeciesSpeciesRokitta, Sebastian D
2 Experimental treatmentExp treatRokitta, Sebastian D
3 SalinitySalRokitta, Sebastian D
4 Temperature, waterTemp°CRokitta, Sebastian D
5 Nitrate[NO3]-µmol/lRokitta, Sebastian D
6 Phosphate[PO4]3-µmol/lRokitta, Sebastian D
7 Radiation, photosynthetically activePARµmol/m2/sRokitta, Sebastian D
8 Light:Dark cycleL:Dhh:hhRokitta, Sebastian D
9 Carbon dioxide, partial pressurepCO2PaRokitta, Sebastian DEstimatedTreatment
10 Carbon dioxide, partial pressurepCO2PaRokitta, Sebastian DCalculated using CO2SYSMeasured
11 Carbon dioxide, partial pressure, standard deviationpCO2 std dev±Rokitta, Sebastian D
12 pHpHRokitta, Sebastian DpH meter, WTW, pH 3000NBS scale
13 pH, standard deviationpH std dev±Rokitta, Sebastian D
14 Alkalinity, totalATµmol/kgRokitta, Sebastian DAlkalinity, Gran titration (Gran, 1950)
15 Alkalinity, total, standard deviationAT std dev±Rokitta, Sebastian D
16 Carbon, inorganic, dissolvedDICµmol/kgRokitta, Sebastian DAuto-analyzer, Technicon Traacs 800
17 Carbon, inorganic, dissolved, standard deviationDIC std dev±Rokitta, Sebastian D
18 Bicarbonate ion[HCO3]-µmol/kgRokitta, Sebastian DCalculated using CO2SYS
19 Bicarbonate ion, standard deviation[HCO3]- std dev±Rokitta, Sebastian D
20 Carbonate ion[CO3]2-µmol/kgRokitta, Sebastian DCalculated using CO2SYS
21 Carbonate ion, standard deviation[CO3]2- std dev±Rokitta, Sebastian D
22 Calcite saturation stateOmega CalRokitta, Sebastian DCalculated using CO2SYS
23 Calcite saturation state, standard deviationOmega Cal std dev±Rokitta, Sebastian D
24 Growth rateµ#/dayRokitta, Sebastian DCalculated, see reference(s)
25 Growth rate, standard deviationµ std dev±Rokitta, Sebastian D
26 Carbon, inorganic, particulate, per cellPIC/cellpg/#Rokitta, Sebastian DMass spectrometer SL 20-20 (SerCon)
27 Particulate inorganic carbon per cell, standard deviationPIC/cell std dev±Rokitta, Sebastian D
28 Carbon, organic, particulate, per cellPOC/cellpg/#Rokitta, Sebastian DMass spectrometer SL 20-20 (SerCon)
29 Particulate organic carbon content per cell, standard deviationPOC cont/cell std dev±Rokitta, Sebastian D
30 Particulate organic nitrogen per cellPON/cellpg/#Rokitta, Sebastian DMass spectrometer SL 20-20 (SerCon)
31 Particulate organic nitrogen per cell, standard deviationPON/cell std dev±Rokitta, Sebastian D
32 Particulate inorganic carbon/particulate organic carbon ratioPIC/POCRokitta, Sebastian DCalculated
33 Particulate inorganic carbon/particulate organic carbon ratio, standard deviationPIC/POC ratio std dev±Rokitta, Sebastian D
34 Carbon, organic, particulate/Nitrogen, organic, particulate ratioPOC/PONRokitta, Sebastian DCalculated
35 Carbon, organic, particulate/Nitrogen, organic, particulate ratio, standard deviationPOC/PON std dev±Rokitta, Sebastian D
36 Particulate organic carbon production per cellPOC prod/cellpg/#/dayRokitta, Sebastian DCalculated
37 Particulate organic carbon, production, standard deviationPOC prod std dev±Rokitta, Sebastian D
38 Particulate inorganic carbon production per cellPIC prod/cellpg/#/dayRokitta, Sebastian DCalculated
39 Particulate inorganic carbon, production, standard deviationPIC prod std dev±Rokitta, Sebastian D
40 Total particulate carbon production per cellTPC prod/cellpg/#/dayRokitta, Sebastian DMass spectrometer SL 20-20 (SerCon)
41 Total particulate carbon production, standard deviationTPC prod std dev±Rokitta, Sebastian D
42 Production of particulate organic nitrogenPON prodpg/#/dayRokitta, Sebastian DCalculated
43 Particulate organic nitrogen production, standard deviationPON prod std dev±Rokitta, Sebastian D
44 Chlorophyll a per cellChl a/cellpg/#Rokitta, Sebastian DPigments, Turner fluorometer
45 Chlorophyll a, standard deviationChl a std dev±Rokitta, Sebastian D
46 Chlorophyll a/particulate organic carbon ratioChl a/POCRokitta, Sebastian D
47 Chlorophyll a/particulate organic carbon ratio, standard deviationChl a/POC std dev±Rokitta, Sebastian D
48 Bicarbonate uptake/net fixation ratio[HCO3]- upt/net fixmol/molRokitta, Sebastian D
49 Bicarbonate uptake/net fixation ratio, standard deviation[HCO3]- upt/net fix std dev±Rokitta, Sebastian D
50 Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
51 pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale; Calculated from means
52 Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Calculated from means
53 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Calculated from means
54 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Calculated from means
55 Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Calculated from means
56 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Calculated from means
57 Carbon, inorganic, dissolvedDICµmol/kgRokitta, Sebastian DCalculated using CO2SYSCalculated from means
58 Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Calculated from means
59 Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Calculated from means
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
536 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Species		2 
Exp treat		3 
Sal		4 
Temp [°C]		5 
[NO3]- [µmol/l]		6 
[PO4]3- [µmol/l]		7 
PAR [µmol/m2/s]		8 
L:D [hh:hh]		9 
pCO2 [Pa]
(Treatment, Estimated)		10 
pCO2 [Pa]
(Measured, Calculated using CO...)		11 
pCO2 std dev [±]		12 
pH
(NBS scale, pH meter, WTW, pH ...)		13 
pH std dev [±]		14 
AT [µmol/kg]
(Alkalinity, Gran titration (G...)		15 
AT std dev [±]		16 
DIC [µmol/kg]
(Auto-analyzer, Technicon Traa...)		17 
DIC std dev [±]		18 
[HCO3]- [µmol/kg]
(Calculated using CO2SYS)		19 
[HCO3]- std dev [±]		20 
[CO3]2- [µmol/kg]
(Calculated using CO2SYS)		21 
[CO3]2- std dev [±]		22 
Omega Cal
(Calculated using CO2SYS)		23 
Omega Cal std dev [±]		24 
µ [#/day]
(Calculated, see reference(s))		25 
µ std dev [±]		26 
PIC/cell [pg/#]
(Mass spectrometer SL 20-20 (S...)		27 
PIC/cell std dev [±]		28 
POC/cell [pg/#]
(Mass spectrometer SL 20-20 (S...)		29 
POC cont/cell std dev [±]		30 
PON/cell [pg/#]
(Mass spectrometer SL 20-20 (S...)		31 
PON/cell std dev [±]		32 
PIC/POC
(Calculated)		33 
PIC/POC ratio std dev [±]		34 
POC/PON
(Calculated)		35 
POC/PON std dev [±]		36 
POC prod/cell [pg/#/day]
(Calculated)		37 
POC prod std dev [±]		38 
PIC prod/cell [pg/#/day]
(Calculated)		39 
PIC prod std dev [±]		40 
TPC prod/cell [pg/#/day]
(Mass spectrometer SL 20-20 (S...)		41 
TPC prod std dev [±]		42 
PON prod [pg/#/day]
(Calculated)		43 
PON prod std dev [±]		44 
Chl a/cell [pg/#]
(Pigments, Turner fluorometer)		45 
Chl a std dev [±]		46 
Chl a/POC		47 
Chl a/POC std dev [±]		48 
[HCO3]- upt/net fix [mol/mol]		49 
[HCO3]- upt/net fix std dev [±]		50 
CSC flag
(Calculated using seacarb afte...)		51 
pH
(Total scale; Calculated from ...)		52 
CO2 [µmol/kg]
(Calculated from means, Calcul...)		53 
pCO2water_SST_wet [µatm]
(Calculated from means, Calcul...)		54 
fCO2water_SST_wet [µatm]
(Calculated from means, Calcul...)		55 
[HCO3]- [µmol/kg]
(Calculated from means, Calcul...)		56 
[CO3]2- [µmol/kg]
(Calculated from means, Calcul...)		57 
DIC [µmol/kg]
(Calculated from means, Calcul...)		58 
Omega Arg
(Calculated from means, Calcul...)		59 
Omega Cal
(Calculated from means, Calcul...)
'Emiliania huxleyi RCC 1216, 2N'pCO2 low32151006.255016:0838.540.40.48.1120.014225420199744191017140.12.13.470.050.630.147.450.5410.350.631.250.08104.000.166.970.314.721.136.561.5511.292.590.790.190.160.010.020.0020.850.01267.9917.60463.73462.081931.17131.122079.902.033.19
'Emiliania huxleyi RCC 1216, 2N'pCO2 high32151006.255016:08101.397.311.17.8080.012238572232222186180.02.81.940.070.740.1611.871.142.350.582.310.290.200.075.950.918.732.071.730.5710.462.311.700.420.180.010.020.0020.860.04267.6940.071055.691051.932202.4174.912317.401.161.82
'Emiliania huxleyi RCC 1216, 2N'pCO2 low32151006.2530016:0838.543.50.28.0830.0402273520181519424134.60.63.330.011.020.1510.811.418.782.341.520.370.820.227.480.2611.032.198.962.7420.004.321.550.440.120.030.010.0030.890.03267.9619.21506.06504.261966.68124.612110.501.933.03
'Emiliania huxleyi RCC 1216, 2N'pCO2 high32151006.2530016:08101.3110.614.67.7410.00223223521743221493668.81.91.670.050.810.1915.082.209.292.032.290.390.650.197.750.6312.273.367.552.4120.495.491.870.540.100.020.010.0020.760.05267.6246.361221.431217.082168.2862.762277.400.971.52
'Emiliania huxleyi RCC 1217, 1N'pCO2 low32151006.255016:0838.535.60.48.1760.005239324211119197821170.82.84.230.070.870.127.700.670.580.121.490.150.070.016.000.136.731.110.510.127.241.211.300.230.190.010.020.0020.870.01268.0615.55409.55408.092003.89159.862179.302.483.88
'Emiliania huxleyi RCC 1217, 1N'pCO2 high32151006.255016:08101.335.61.97.8230.022239832249232190483.51.52.030.040.750.1210.042.340.430.412.070.560.060.035.840.567.522.150.320.317.812.211.550.490.130.010.010.0030.870.03267.7039.311035.511031.812210.6576.952326.901.191.87
'Emiliania huxleyi RCC 1217, 1N'pCO2 low32151006.2530016:0838.595.70.48.1770.019238010209624196718169.53.44.200.080.180.209.651.900.610.351.630.350.060.046.920.3311.402.940.730.4312.123.111.930.530.090.020.010.0030.890.03268.0615.46407.25405.791992.67158.972167.102.473.86
'Emiliania huxleyi RCC 1217, 1N'pCO2 high32151006.2530016:08101.335.55.97.8180.03824022122963421991481.77.11.980.170.850.1410.581.050.510.161.930.200.050.026.710.308.961.740.430.159.371.831.630.320.070.000.010.0010.830.05267.7039.361036.981033.282214.3477.092330.801.201.87
ReferencepCO2 low32151006.2516:0838.540.91.28.1340.01024111321342520303156.74.93.880.12268.0117.89471.30469.622055.63146.182219.702.273.55
ReferencepCO2 high32151006.2516:08101.3100.910.47.7700.0252396172269452171478.27.71.900.19267.6544.421170.381166.212227.6569.132341.201.071.68