Data Description

Citation:
Borchard, Corinna; Borges, Alberto Vieira; Händel, Nicole; Engel, Anja (2012): Seawater carbonate chemistry and biological processes of Emiliania huxleyi (PML B92/11) during experiments, 2011. doi:10.1594/PANGAEA.774802
Reference(s):
Borchard, Corinna; Borges, Alberto Vieira; Händel, Nicole; Engel, Anja (2011): Biogeochemical response of Emiliania huxleyi (PML B92/11) to elevated CO2 and temperature under phosphorous limitation: A chemostat study. Journal of Experimental Marine Biology and Ecology, 410, 61-71, doi:10.1016/j.jembe.2011.10.004 *
Abstract:
The present study investigates the combined effect of phosphorous limitation, elevated partial pressure of CO2 (pCO2) and temperature on a calcifying strain of Emiliania huxleyi (PML B92/11) by means of a fully controlled continuous culture facility. Two levels of phosphorous limitation were consecutively applied by renewal of culture media (N:P = 26) at dilution rates (D) of 0.3 d-  and 0.1 d-1. CO2 and temperature conditions were 300, 550 and 900 µatm pCO2 at 14 °C and 900 µatm pCO2 at 18 °C. In general, the steady state cell density and particulate organic carbon (POC) production increased with pCO2, yielding significantly higher concentrations in cultures grown at 900 µatm pCO2 compared to 300 and 550 µatm pCO2. At 900 µatm pCO2, elevation of temperature as expected for a greenhouse ocean, further increased cell densities and POC concentrations. In contrast to POC concentration, C-quotas (pmol C cell-1) were similar at D = 0.3 d-1 in all cultures. At D = 0.1 d-1, a reduction of C-quotas by up to 15% was observed in the 900 µatm pCO2 at 18 °C culture. As a result of growth rate reduction, POC:PON:POP ratios deviated strongly from the Redfield ratio, primarily due to an increase in POC. Ratios of particulate inorganic and organic carbon (PIC:POC) ranged from 0.14 to 0.18 at D = 0.3 d-1, and from 0.11 to 0.17 at D = 0.1 d-1, with variations primarily induced by the changes in POC. At D = 0.1 d-1, cell volume was reduced by up to 22% in cultures grown at 900 µatm pCO2. Our results indicate that changes in pCO2, temperature and phosphorus supply affect cell density, POC concentration and size of E. huxleyi (PML B92/11) to varying degrees, and will likely impact bloom development as well as biogeochemical cycling in a greenhouse ocean.
Project(s):
European Project on Ocean Acidification (EPOCA) *
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Experimental treatment *Exp trtmEngel, Anja *
2Sample ID *IDEngel, Anja *
3Salinity *SalEngel, Anja *Salinometer - Tropic Marin Sea Salt, Dr. Biener GmbH, Germany *
4Temperature, water *Temp°CEngel, Anja *WTW 340i pH-analyzer and WTW SenTix 81-electrode *
5Nitrate *NO3µmol/lEngel, Anja *Spectrophotometry *
6Phosphate *PO4µmol/lEngel, Anja *Spectrophotometry *
7Light:Dark cycle *L:Dhh:hhEngel, Anja *
8Radiation, photosynthetically active *PARµE/m2/sEngel, Anja *
9Total alkalinity *TAµmol/kgEngel, Anja *measured *
10pH *pHEngel, Anja *WTW 340i pH-analyzer and WTW SenTix 81-electrode *NBS scale
11Total carbon *TCµmol/kgEngel, Anja *calculated using CO2SYS *
12Partial pressure of Carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmEngel, Anja *Infrared gas analyzer (LICOR LI-6252) *
13Carbon dioxide *CO2µmol/kgEngel, Anja *calculated using CO2SYS *
14Bicarbonate ion concentration *HCO3-µmol/kgEngel, Anja *calculated using CO2SYS *
15Carbonate ion concentration *CO3**2-µmol/kgEngel, Anja *calculated using CO2SYS *
16Calcite saturation state *SI CalEngel, Anja *calculated using CO2SYS *
17Revelle factor *REngel, Anja *
18Experiment day *Exp daydayEngel, Anja *
19Nitrogen, inorganic, dissolved *DINµmol/lEngel, Anja *Element analyser CNS, EURO EA *
20Nitrogen, inorganic, dissolved *DINµmol/lEngel, Anja *Element analyser CNS, EURO EA *
21Nitrogen, organic *N orgµmol/lEngel, Anja *Element analyser CNS, EURO EA *
22Particulate organic nitrogen production *PON prodµmol N/l*dayEngel, Anja *calculated, see reference(s) *
23Particulate organic nitrogen per cell *PON cellpmol/CEngel, Anja *calculated, see reference(s) *
24Production of particulate organic nitrogen *PON prodpg/cell*dayEngel, Anja *calculated, see reference(s) *
25Phosphorus, inorganic *P inorgµmol/lEngel, Anja *Colorimetry *
26Phosphorus, inorganic *P inorgµmol/lEngel, Anja *Colorimetry *
27Phosphorus, organic, particulate *POPµmol/lEngel, Anja *Colorimetry *
28Particulate organic phosphorus production *POP prodµmol P/l*dayEngel, Anja *calculated, see reference(s) *
29Particulate organic phosphorus per cell *POP cellpmol/cellEngel, Anja *calculated, see reference(s) *
30Production of particulate organic phosphorus *POP prodpmol/cell/dayEngel, Anja *calculated, see reference(s) *
31Carbon, organic, particulate *POCµmol/lEngel, Anja *Element analyser CNS, EURO EA *
32Particulate organic carbon production *POC prodµmol C/l*dayEngel, Anja *calculated, see reference(s) *
33Particulate organic carbon per cell *POC cellpmol/cellEngel, Anja *Element analyser CNS, EURO EA *
34Particulate organic carbon production per cell *POC prod cellµmol C/cell*dayEngel, Anja *calculated, see reference(s) *
35Particulate organic carbon/particulate organic nitrogen ratio *POC/PONEngel, Anja *calculated, see reference(s) *
36Particulate organic carbon/particulate organic phosphorus ratio *POC/POPEngel, Anja *calculated, see reference(s) *
37Particulate organic nitrogen/particulate organic phosphorus ratio *PON/POPEngel, Anja *calculated, see reference(s) *
38Carbonate system computation flag *CSC flagEngel, Anja *calculated using seacarb by Anne-Marin Nisumaa *
39pH *pHEngel, Anja *calculated using seacarb by Anne-Marin Nisumaa *Total scale
40Carbon dioxide *CO2µmol/kgEngel, Anja *calculated using seacarb by Anne-Marin Nisumaa *
41Partial pressure of Carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmEngel, Anja *calculated using seacarb by Anne-Marin Nisumaa *
42Fugacity of Carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmEngel, Anja *calculated using seacarb by Anne-Marin Nisumaa *
43Bicarbonate ion concentration *HCO3-µmol/kgEngel, Anja *calculated using seacarb by Anne-Marin Nisumaa *
44Carbonate ion concentration *CO3**2-µmol/kgEngel, Anja *calculated using seacarb by Anne-Marin Nisumaa *
45Total carbon *TCµmol/kgEngel, Anja *calculated using seacarb by Anne-Marin Nisumaa *
46Total alkalinity *TAµmol/kgEngel, Anja *calculated using seacarb by Anne-Marin Nisumaa *
47Aragonite saturation state *SI AragEngel, Anja *calculated using seacarb by Anne-Marin Nisumaa *
48Calcite saturation state *SI CalEngel, Anja *calculated using seacarb by Anne-Marin Nisumaa *
License:
CC-BY Creative Commons Attribution 3.0 Unported
Size:
1068 data points

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