/* DATA DESCRIPTION: Citation: Hu, Marian Y; Tseng, Yung-Che; Stumpp, Meike; Gutowska, Magdalena A; Kiko, Rainer; Lucassen, Magnus; Melzner, Frank (2011): Seawater carbonate chemistry, mass, length and gene expression of Sepia officinalis during experiments, 2011. PANGAEA, https://doi.org/10.1594/PANGAEA.774787, Supplement to: Hu, MY et al. (2011): Elevated seawater pCO2 differentially affects branchial acid-base transporters over the course of development in the cephalopod Sepia officinalis. American Journal of Physiology, 300(5), R1100-R1114, https://doi.org/10.1152/ajpregu.00653.2010 Abstract: The specific transporters involved in maintenance of blood pH homeostasis in cephalopod molluscs have not been identified to date. Using in situ hybridization and immuno histochemical methods, we demonstrate that Na+/K+-ATPase (soNKA), a V-type H+-ATPase (soV-HA), and Na+/HCO3- cotransporter (soNBC) are co-localized in NKA-rich cells in the gills of Sepia officinalis. mRNA expression patterns of these transporters and selected metabolic genes were examined in response to moderately elevated seawater pCO2 (0.16 and 0.35 kPa) over a time-course of six weeks in different ontogenetic stages. The applied CO2 concentrations are relevant for ocean acidification scenarios projected for the coming decades. We determined strong expression changes in late stage embryos and hatchlings, with one to three log2-fold reductions in soNKA, soNBCe, socCAII and COX. In contrast, no hypercapnia induced changes in mRNA expression were observed in juveniles during both short- and long-term exposure. However a transiently increased demand of ion regulatory demand was evident during the initial acclimation reaction to elevated seawater pCO2. Gill Na+/K+-ATPase activity and protein concentration were increased by approximately 15% in during short (2-11 day), but not long term (42 day) exposure. Our findings support the hypothesis that the energy budget of adult cephalopods is not significantly compromised during long-term exposure to moderate environmental hypercapnia. However, the down regulation of ion-regulatory and metabolic genes in late stage embryos, taken together with a significant reduction in somatic growth, indicates that cephalopod early life stages are challenged by elevated seawater pCO2. Keyword(s): Acid-base regulation; Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gene expression (incl. proteomics); Growth/Morphology; Laboratory experiment; Mollusca; North Atlantic; Sepia officinalis; Single species; Temperate Project(s): Biological Impacts of Ocean Acidification (BIOACID) (URI: https://www.bioacid.de/) European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS) (URI: https://eur-oceans.eu/) European Project on Ocean Acidification (EPOCA) (URI: https://en.wikipedia.org/wiki/European_Project_on_Ocean_Acidification) Ocean Acidification International Coordination Centre (OA-ICC) (URI: https://www.iaea.org/services/oa-icc/science-and-collaboration/data-access-and-management) Funding: Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification (URI: https://cordis.europa.eu/project/id/211384) Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis (URI: https://cordis.europa.eu/project/id/511106) Comment: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). Parameter(s): Species (Species) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Comment (Comment) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Experimental treatment (Exp treat) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Experiment day [day] (Exp day) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Salinity (Sal) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Salinity, standard deviation [±] (Sal std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Temperature, water [°C] (Temp) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Temperature, standard deviation [±] (T std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) pH (pH) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: WTW 340i pH-analyzer and WTW SenTix 81-electrode * COMMENT: NBS scale pH, standard deviation [±] (pH std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Aragonite saturation state (Omega Arg) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: Calculated using CO2SYS (URI: http://cdiac.ornl.gov/oceans/co2rprt.html) Aragonite saturation state, standard deviation [±] (Omega Arg std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Carbon dioxide, partial pressure [Pa] (pCO2) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: Calculated using CO2SYS (URI: http://cdiac.ornl.gov/oceans/co2rprt.html) Carbon dioxide, partial pressure, standard deviation [±] (pCO2 std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Alkalinity, total [µmol/kg] (AT) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: Potentiometric open-cell titration (URI: https://doi.org/10.1016/S0304-4203(02)00133-0) Alkalinity, total, standard deviation [±] (AT std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Sepia officinalis, mass [g] (S. officinalis m) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: Precision scale (Sartorius TE64, Sartorius AG, Germany) Sepia officinalis, mass, standard deviation [±] (S. officinalis m std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Sepia officinalis, length, mantle [mm] (S. officinalis l) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: Stereomicroscopy (Leica) Sepia officinalis, length, mantle, standard deviation [±] (S. officinalis l std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Sodium/Potassium adenosine triphosphatase gene expression (Na/K-ATPase) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: see reference(s) Sodium/Potassium adenosine triphosphatase gene expression, standard deviation [±] (Na/K-ATPase std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Sodium/bicarbonate cotransporter gene expression (Na/HCO3 cotransp) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: see reference(s) Sodium/bicarbonate cotransporter gene expression, standard deviation [±] (Na/HCO3 cotransp std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Sodium driven chlorine/bicarbonate exchanger gene expression (Na-driven Cl/HCO3 exchanger) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: see reference(s) Sodium driven chlorine/bicarbonate exchanger gene expression, standard deviation [±] (Na-driven Cl/HCO3 exchanger std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Carbonic anhydrase gene expression (CA) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: see reference(s) Carbonic anhydrase gene expression, standard deviation [±] (CA std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Adenosine triphosphate synthase gene expression (ATP-synth) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: see reference(s) Adenosine triphosphate synthase gene expression, standard deviation [±] (ATP-synth std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Cytochrome P-450 gene expression (CYP 450) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: see reference(s) Cytochrome P-450 gene expression, standard deviation [±] (CYP 450 std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Cytochrome-c-oxydase gene expression (COX) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: see reference(s) Cytochrome-c-oxydase gene expression, standard deviation [±] (COX std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Octopine dehydrogenase gene expression (ODH) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: see reference(s) Octopine dehydrogenase gene expression, standard deviation [±] (ODH std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Sodium/Potassium adenosine triphosphatase maximum (Na/K-ATPase max) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: see reference(s) Sodium/Potassium adenosine triphosphatase maximum, standard deviation [±] (Na/K-ATPase max std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Sodium/Potassium adenosine triphosphatase relative protein concentration [%] (Na/K-ATPase protein) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: see reference(s) Sodium/Potassium adenosine triphosphatase relative protein concentration, standard deviation [±] (Na/K-ATPase protein std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Sodium/Potassium adenosine triphosphatase relative mRNA expression [%] (Na/K-ATPase mRNA) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: see reference(s) Sodium/Potassium adenosine triphosphatase relative mRNA expression, standard deviation [±] (Na/K-ATPase mRNA std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Sepia officinalis, perivitelline fluid, pH (S. officinalis PVF pH) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: WTW 340i pH-analyzer and WTW SenTix 81-electrode Sepia officinalis, perivitelline fluid, pCO2 [kPa] (S. officinalis PVF pCO2) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: Calculated using CO2SYS (URI: http://cdiac.ornl.gov/oceans/co2rprt.html) Gene name (Gene) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Sepia officinalis, mRNA gene expression (S. officinalis mRNA express) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: see reference(s) Sepia officinalis, mRNA gene expression, standard deviation [±] (S. officinalis mRNA express std dev) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) Sepia officinalis, mRNA gene expression change [%] (S. officinalis mRNA express change) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: see reference(s) Carbonate system computation flag (CSC flag) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) pH (pH) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: Total scale Carbon dioxide [µmol/kg] (CO2) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (pCO2water_SST_wet) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Fugacity of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (fCO2water_SST_wet) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Bicarbonate ion [µmol/kg] ([HCO3]-) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Carbonate ion [µmol/kg] ([CO3]2-) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Carbon, inorganic, dissolved [µmol/kg] (DIC) * PI: Hu, Marian Y (https://orcid.org/0000-0002-8914-139X, m.hu@physiologie.uni-kiel.de) * METHOD/DEVICE: Calculated using CO2SYS (URI: http://cdiac.ornl.gov/oceans/co2rprt.html) Aragonite saturation state (Omega Arg) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Calcite saturation state (Omega Cal) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) License: Creative Commons Attribution 3.0 Unported (CC-BY-3.0) (URI: https://creativecommons.org/licenses/by/3.0/) Status: Curation Level: Enhanced curation (URI: https://wiki.pangaea.de/wiki/Curation_levels) Size: 2979 data points */ Species Comment Exp treat Exp day [day] Sal Sal std dev [±] Temp [°C] T std dev [±] pH (NBS scale, WTW 340i pH-analyz...) pH std dev [±] Omega Arg (Calculated using CO2SYS) Omega Arg std dev [±] pCO2 [Pa] (Calculated using CO2SYS) pCO2 std dev [±] AT [µmol/kg] (Potentiometric open-cell titr...) AT std dev [±] S. officinalis m [g] (Precision scale (Sartorius TE...) S. officinalis m std dev [±] S. officinalis l [mm] (Stereomicroscopy (Leica)) S. officinalis l std dev [±] Na/K-ATPase (see reference(s)) Na/K-ATPase std dev [±] Na/HCO3 cotransp (see reference(s)) Na/HCO3 cotransp std dev [±] Na-driven Cl/HCO3 exchanger (see reference(s)) Na-driven Cl/HCO3 exchanger std dev [±] CA (see reference(s)) CA std dev [±] ATP-synth (see reference(s)) ATP-synth std dev [±] CYP 450 (see reference(s)) CYP 450 std dev [±] COX (see reference(s)) COX std dev [±] ODH (see reference(s)) ODH std dev [±] Na/K-ATPase max (see reference(s)) Na/K-ATPase max std dev [±] Na/K-ATPase protein [%] (see reference(s)) Na/K-ATPase protein std dev [±] Na/K-ATPase mRNA [%] (see reference(s)) Na/K-ATPase mRNA std dev [±] S. officinalis PVF pH (WTW 340i pH-analyzer and WTW ...) S. officinalis PVF pCO2 [kPa] (Calculated using CO2SYS) Gene S. officinalis mRNA express (see reference(s)) S. officinalis mRNA express std dev [±] S. officinalis mRNA express change [%] (see reference(s)) CSC flag (Calculated using seacarb afte...) pH (Total scale, Calculated using...) CO2 [µmol/kg] (Calculated using seacarb afte...) pCO2water_SST_wet [µatm] (Calculated using seacarb afte...) fCO2water_SST_wet [µatm] (Calculated using seacarb afte...) [HCO3]- [µmol/kg] (Calculated using seacarb afte...) [CO3]2- [µmol/kg] (Calculated using seacarb afte...) DIC [µmol/kg] (Calculated using CO2SYS) Omega Arg (Calculated using seacarb afte...) Omega Cal (Calculated using seacarb afte...) Sepia officinalis Embryos Control 34.94 0.10 14.60 0.47 7.96 0.02 1.69 0.06 70.97 4.13 2413.81 49.10 0.1694 0.0088 6905 187 26 7.85 26.80 709.20 706.65 2143.08 110.12 2280.00 1.68 2.62 Sepia officinalis Embryos pCO2 0.14 kPa 34.92 0.07 14.62 0.47 7.63 0.02 0.84 0.01 164.04 24.48 2431.89 56.12 0.1703 0.0049 7010 159 -0.0387 0.1377 -1.2283 0.0283 -0.2473 0.0023 0.5241 0.1874 0.1243 0.1249 0.2395 0.0003 0.0078 0.3900 0.0804 0.0013 26 7.51 61.92 1639.08 1633.21 2297.40 54.78 2414.10 0.84 1.31 Sepia officinalis Embryos pCO2 0.4 kPa 34.90 0.05 14.62 0.45 7.28 0.03 0.39 0.02 371.35 28.52 2415.16 43.00 0.1619 0.0048 6328 167 -0.7702 0.3894 -2.1368 0.1533 0.0670 0.1345 -1.2362 0.2277 -0.2165 0.1171 -0.3318 0.3055 0.9054 0.4672 -0.1448 0.3472 26 7.17 140.26 3712.69 3699.39 2352.88 25.36 2518.50 0.39 0.60 Sepia officinalis hatchling Control 34.94 0.10 14.60 0.47 7.96 0.02 1.69 0.06 70.97 4.13 2413.81 49.10 0.2212 0.0077 7378 196 26 7.85 26.80 709.20 706.65 2143.08 110.12 2280.00 1.68 2.62 Sepia officinalis hatchling pCO2 0.14 kPa 34.92 0.07 14.62 0.47 7.63 0.02 0.84 0.01 164.04 24.48 2431.89 56.12 0.2263 0.0084 7366 110 -0.0001 0.4245 -0.5671 0.4429 0.0646 0.1562 -0.1860 0.3805 0.0412 0.1066 -0.1564 0.6162 0.2872 0.2994 -0.7522 0.3664 26 7.51 61.92 1639.08 1633.21 2297.40 54.78 2414.10 0.84 1.31 Sepia officinalis hatchling pCO2 0.4 kPa 34.90 0.05 14.62 0.45 7.28 0.03 0.39 0.02 371.35 28.52 2415.16 43.00 0.1915 0.0059 6775 650 -0.9710 0.4958 -2.9585 0.1603 0.0357 0.1576 -1.5332 0.2074 -0.2620 0.0886 -0.8383 0.3169 -1.2530 0.2661 -0.0849 0.2957 26 7.17 140.26 3712.69 3699.39 2352.88 25.36 2518.50 0.39 0.60 Sepia officinalis juvenile Control 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile pCO2 0.4 kPa CO2 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 -0.0364 -0.0647 -0.0234 -0.0287 -0.0755 -0.1326 -0.2038 -0.0797 -0.1620 -0.0621 -0.1245 -0.0880 -0.2017 -0.2067 -0.2826 -0.0872 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile Control 2 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 94.8336 3.8640 58.633 7.860 79.629 7.400 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 11 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 83.1815 3.5260 52.137 6.980 75.851 6.310 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 42 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 87.8809 4.6370 96.848 1.220 89.928 6.330 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile pCO2 0.4 kPa CO2 2 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 112.5289 4.2790 70.732 4.660 74.621 3.680 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile pCO2 0.4 kPa CO2 11 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 101.7908 4.4380 77.034 3.350 73.959 5.650 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile pCO2 0.4 kPa CO2 42 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 93.2566 3.6990 100.000 1.970 100.000 8.920 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis embryos final sage of development (stage 30) Control 34.94 0.10 14.60 0.47 7.96 0.02 1.69 0.06 70.97 4.13 2413.81 49.10 7.316 0.292 26 7.85 26.80 709.20 706.65 2143.08 110.12 2280.00 1.68 2.62 Sepia officinalis embryos final sage of development (stage 30) Control 34.94 0.10 14.60 0.47 7.96 0.02 1.69 0.06 70.97 4.13 2413.81 49.10 7.352 0.269 26 7.85 26.80 709.20 706.65 2143.08 110.12 2280.00 1.68 2.62 Sepia officinalis embryos final sage of development (stage 30) Control 34.94 0.10 14.60 0.47 7.96 0.02 1.69 0.06 70.97 4.13 2413.81 49.10 7.431 0.231 26 7.85 26.80 709.20 706.65 2143.08 110.12 2280.00 1.68 2.62 Sepia officinalis embryos final sage of development (stage 30) Control 34.94 0.10 14.60 0.47 7.96 0.02 1.69 0.06 70.97 4.13 2413.81 49.10 7.372 0.252 26 7.85 26.80 709.20 706.65 2143.08 110.12 2280.00 1.68 2.62 Sepia officinalis embryos final sage of development (stage 30) Control 34.94 0.10 14.60 0.47 7.96 0.02 1.69 0.06 70.97 4.13 2413.81 49.10 7.407 0.255 26 7.85 26.80 709.20 706.65 2143.08 110.12 2280.00 1.68 2.62 Sepia officinalis embryos final sage of development (stage 30) Control 34.94 0.10 14.60 0.47 7.96 0.02 1.69 0.06 70.97 4.13 2413.81 49.10 7.424 0.233 26 7.85 26.80 709.20 706.65 2143.08 110.12 2280.00 1.68 2.62 Sepia officinalis embryos final sage of development (stage 30) Control 34.94 0.10 14.60 0.47 7.96 0.02 1.69 0.06 70.97 4.13 2413.81 49.10 7.449 0.212 26 7.85 26.80 709.20 706.65 2143.08 110.12 2280.00 1.68 2.62 Sepia officinalis embryos final sage of development (stage 30) Control 34.94 0.10 14.60 0.47 7.96 0.02 1.69 0.06 70.97 4.13 2413.81 49.10 7.382 0.263 26 7.85 26.80 709.20 706.65 2143.08 110.12 2280.00 1.68 2.62 Sepia officinalis embryos final sage of development (stage 30) Control 34.94 0.10 14.60 0.47 7.96 0.02 1.69 0.06 70.97 4.13 2413.81 49.10 7.403 0.234 26 7.85 26.80 709.20 706.65 2143.08 110.12 2280.00 1.68 2.62 Sepia officinalis embryos final sage of development (stage 30) Control 34.94 0.10 14.60 0.47 7.96 0.02 1.69 0.06 70.97 4.13 2413.81 49.10 7.322 0.300 26 7.85 26.80 709.20 706.65 2143.08 110.12 2280.00 1.68 2.62 Sepia officinalis embryos final sage of development (stage 30) Control 34.94 0.10 14.60 0.47 7.96 0.02 1.69 0.06 70.97 4.13 2413.81 49.10 7.329 0.283 26 7.85 26.80 709.20 706.65 2143.08 110.12 2280.00 1.68 2.62 Sepia officinalis embryos final sage of development (stage 30) Control 34.94 0.10 14.60 0.47 7.96 0.02 1.69 0.06 70.97 4.13 2413.81 49.10 7.394 0.266 26 7.85 26.80 709.20 706.65 2143.08 110.12 2280.00 1.68 2.62 Sepia officinalis embryos final sage of development (stage 30) Control 34.94 0.10 14.60 0.47 7.96 0.02 1.69 0.06 70.97 4.13 2413.81 49.10 7.444 0.224 26 7.85 26.80 709.20 706.65 2143.08 110.12 2280.00 1.68 2.62 Sepia officinalis embryos final sage of development (stage 30) Control 34.94 0.10 14.60 0.47 7.96 0.02 1.69 0.06 70.97 4.13 2413.81 49.10 7.260 0.330 26 7.85 26.80 709.20 706.65 2143.08 110.12 2280.00 1.68 2.62 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.14 kPa 34.92 0.07 14.62 0.47 7.63 0.02 0.84 0.01 164.04 24.48 2431.89 56.12 7.210 0.391 26 7.51 61.92 1639.08 1633.21 2297.40 54.78 2414.10 0.84 1.31 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.14 kPa 34.92 0.07 14.62 0.47 7.63 0.02 0.84 0.01 164.04 24.48 2431.89 56.12 7.228 0.419 26 7.51 61.92 1639.08 1633.21 2297.40 54.78 2414.10 0.84 1.31 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.14 kPa 34.92 0.07 14.62 0.47 7.63 0.02 0.84 0.01 164.04 24.48 2431.89 56.12 7.006 0.649 26 7.51 61.92 1639.08 1633.21 2297.40 54.78 2414.10 0.84 1.31 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.14 kPa 34.92 0.07 14.62 0.47 7.63 0.02 0.84 0.01 164.04 24.48 2431.89 56.12 7.213 0.438 26 7.51 61.92 1639.08 1633.21 2297.40 54.78 2414.10 0.84 1.31 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.14 kPa 34.92 0.07 14.62 0.47 7.63 0.02 0.84 0.01 164.04 24.48 2431.89 56.12 7.333 0.323 26 7.51 61.92 1639.08 1633.21 2297.40 54.78 2414.10 0.84 1.31 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.14 kPa 34.92 0.07 14.62 0.47 7.63 0.02 0.84 0.01 164.04 24.48 2431.89 56.12 7.294 0.339 26 7.51 61.92 1639.08 1633.21 2297.40 54.78 2414.10 0.84 1.31 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.14 kPa 34.92 0.07 14.62 0.47 7.63 0.02 0.84 0.01 164.04 24.48 2431.89 56.12 7.134 0.507 26 7.51 61.92 1639.08 1633.21 2297.40 54.78 2414.10 0.84 1.31 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.14 kPa 34.92 0.07 14.62 0.47 7.63 0.02 0.84 0.01 164.04 24.48 2431.89 56.12 7.213 0.428 26 7.51 61.92 1639.08 1633.21 2297.40 54.78 2414.10 0.84 1.31 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.14 kPa 34.92 0.07 14.62 0.47 7.63 0.02 0.84 0.01 164.04 24.48 2431.89 56.12 7.334 0.328 26 7.51 61.92 1639.08 1633.21 2297.40 54.78 2414.10 0.84 1.31 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.14 kPa 34.92 0.07 14.62 0.47 7.63 0.02 0.84 0.01 164.04 24.48 2431.89 56.12 7.280 0.369 26 7.51 61.92 1639.08 1633.21 2297.40 54.78 2414.10 0.84 1.31 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.14 kPa 34.92 0.07 14.62 0.47 7.63 0.02 0.84 0.01 164.04 24.48 2431.89 56.12 7.106 0.538 26 7.51 61.92 1639.08 1633.21 2297.40 54.78 2414.10 0.84 1.31 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.14 kPa 34.92 0.07 14.62 0.47 7.63 0.02 0.84 0.01 164.04 24.48 2431.89 56.12 7.111 0.533 26 7.51 61.92 1639.08 1633.21 2297.40 54.78 2414.10 0.84 1.31 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.14 kPa 34.92 0.07 14.62 0.47 7.63 0.02 0.84 0.01 164.04 24.48 2431.89 56.12 7.239 0.433 26 7.51 61.92 1639.08 1633.21 2297.40 54.78 2414.10 0.84 1.31 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.14 kPa 34.92 0.07 14.62 0.47 7.63 0.02 0.84 0.01 164.04 24.48 2431.89 56.12 7.060 0.615 26 7.51 61.92 1639.08 1633.21 2297.40 54.78 2414.10 0.84 1.31 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.4 kPa 34.90 0.05 14.62 0.45 7.28 0.03 0.39 0.02 371.35 28.52 2415.16 43.00 7.057 0.808 26 7.17 140.26 3712.69 3699.39 2352.88 25.36 2518.50 0.39 0.60 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.4 kPa 34.90 0.05 14.62 0.45 7.28 0.03 0.39 0.02 371.35 28.52 2415.16 43.00 7.047 1.217 26 7.17 140.26 3712.69 3699.39 2352.88 25.36 2518.50 0.39 0.60 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.4 kPa 34.90 0.05 14.62 0.45 7.28 0.03 0.39 0.02 371.35 28.52 2415.16 43.00 7.027 0.714 26 7.17 140.26 3712.69 3699.39 2352.88 25.36 2518.50 0.39 0.60 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.4 kPa 34.90 0.05 14.62 0.45 7.28 0.03 0.39 0.02 371.35 28.52 2415.16 43.00 7.118 0.713 26 7.17 140.26 3712.69 3699.39 2352.88 25.36 2518.50 0.39 0.60 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.4 kPa 34.90 0.05 14.62 0.45 7.28 0.03 0.39 0.02 371.35 28.52 2415.16 43.00 6.996 0.666 26 7.17 140.26 3712.69 3699.39 2352.88 25.36 2518.50 0.39 0.60 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.4 kPa 34.90 0.05 14.62 0.45 7.28 0.03 0.39 0.02 371.35 28.52 2415.16 43.00 6.957 0.771 26 7.17 140.26 3712.69 3699.39 2352.88 25.36 2518.50 0.39 0.60 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.4 kPa 34.90 0.05 14.62 0.45 7.28 0.03 0.39 0.02 371.35 28.52 2415.16 43.00 7.167 0.767 26 7.17 140.26 3712.69 3699.39 2352.88 25.36 2518.50 0.39 0.60 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.4 kPa 34.90 0.05 14.62 0.45 7.28 0.03 0.39 0.02 371.35 28.52 2415.16 43.00 6.786 0.620 26 7.17 140.26 3712.69 3699.39 2352.88 25.36 2518.50 0.39 0.60 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.4 kPa 34.90 0.05 14.62 0.45 7.28 0.03 0.39 0.02 371.35 28.52 2415.16 43.00 7.054 0.686 26 7.17 140.26 3712.69 3699.39 2352.88 25.36 2518.50 0.39 0.60 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.4 kPa 34.90 0.05 14.62 0.45 7.28 0.03 0.39 0.02 371.35 28.52 2415.16 43.00 7.016 26 7.17 140.26 3712.69 3699.39 2352.88 25.36 2518.50 0.39 0.60 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.4 kPa 34.90 0.05 14.62 0.45 7.28 0.03 0.39 0.02 371.35 28.52 2415.16 43.00 7.069 26 7.17 140.26 3712.69 3699.39 2352.88 25.36 2518.50 0.39 0.60 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.4 kPa 34.90 0.05 14.62 0.45 7.28 0.03 0.39 0.02 371.35 28.52 2415.16 43.00 7.007 26 7.17 140.26 3712.69 3699.39 2352.88 25.36 2518.50 0.39 0.60 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.4 kPa 34.90 0.05 14.62 0.45 7.28 0.03 0.39 0.02 371.35 28.52 2415.16 43.00 7.040 26 7.17 140.26 3712.69 3699.39 2352.88 25.36 2518.50 0.39 0.60 Sepia officinalis embryos final sage of development (stage 30) pCO2 0.4 kPa 34.90 0.05 14.62 0.45 7.28 0.03 0.39 0.02 371.35 28.52 2415.16 43.00 7.100 26 7.17 140.26 3712.69 3699.39 2352.88 25.36 2518.50 0.39 0.60 Sepia officinalis juvenile Control 2 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 NKA 7.807 0.725 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 2 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 NBC 2.982 0.298 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 2 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 NDCBE 0.261 0.038 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 2 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 CA 3.327 0.368 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 2 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 ATP-Synthase 8.054 0.637 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 2 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 CYP 450 0.430 0.032 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 2 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 COX 8.388 2.409 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 2 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 ODH 0.997 0.074 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile 0.3 kPa 2 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 NKA 7.316 0.361 -6.289 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 2 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 NBC 2.804 0.253 -5.970 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 2 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 NDCBE 0.207 0.010 -20.770 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 2 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 CA 3.245 0.222 -2.471 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 2 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 ATP-Synthase 8.324 0.222 3.345 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 2 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 CYP 450 0.438 0.034 1.957 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 2 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 COX 4.158 0.613 -50.432 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 2 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 ODH 1.009 0.065 1.205 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile Control 10 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 NKA 7.437 0.619 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 10 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 NBC 2.367 0.118 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 10 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 NDCBE 0.260 0.039 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 10 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 CA 3.472 0.339 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 10 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 ATP-Synthase 8.452 0.477 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 10 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 CYP 450 0.382 0.036 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 10 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 COX 7.724 2.089 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 10 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 ODH 0.984 0.101 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile 0.3 kPa 10 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 NKA 7.251 0.554 -2.494 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 10 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 NBC 2.329 0.130 -1.609 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 10 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 NDCBE 0.247 0.014 -5.096 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 10 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 CA 3.014 0.325 -13.174 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 10 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 ATP-Synthase 7.555 0.548 -10.618 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 10 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 CYP 450 0.332 0.030 -13.050 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 10 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 COX 6.350 2.742 -17.792 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 10 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 ODH 0.902 0.099 -8.269 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile Control 42 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 NKA 8.820 0.620 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 42 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 NBC 3.860 0.190 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 42 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 NDCBE 0.280 0.050 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 42 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 CA 4.450 0.170 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 42 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 ATP-Synthase 7.390 0.400 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 42 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 CYP 450 0.360 0.040 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 42 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 COX 6.960 1.600 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile Control 42 33.08 0.20 14.81 0.20 8.21 0.08 3.53 0.53 50.26 8.76 2827.00 129.08 ODH 1.250 0.040 26 8.09 16.77 441.88 440.30 2340.56 204.87 2562.20 3.16 4.94 Sepia officinalis juvenile 0.3 kPa 42 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 NKA 9.804 0.880 11.201 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 42 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 NBC 4.172 0.300 8.012 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 42 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 NDCBE 0.311 0.010 11.049 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 42 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 CA 4.896 0.260 10.120 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 42 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 ATP-Synthase 8.592 0.650 16.229 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 42 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 CYP 450 0.467 0.050 29.561 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 42 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 COX 12.293 3.580 76.681 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97 Sepia officinalis juvenile 0.3 kPa 42 33.09 0.21 14.83 0.21 7.39 0.09 0.75 0.11 308.94 37.40 3127.53 141.91 ODH 1.263 0.120 1.044 26 7.27 143.71 3789.74 3776.21 3033.51 40.17 3217.40 0.62 0.97