Data Description

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Citation:
Hu, MY et al. (2011): Seawater carbonate chemistry, mass, length and gene expression of Sepia officinalis during experiments, 2011. doi:10.1594/PANGAEA.774787,
Supplement to: Hu, Marian Y; Tseng, Yung-Che; Stumpp, Meike; Gutowska, Magdalena A; Kiko, Rainer; Lucassen, Magnus; Melzner, Frank (2011): Elevated seawater pCO2 differentially affects branchial acid-base transporters over the course of development in the cephalopod Sepia officinalis. American Journal of Physiology, 300(5), R1100-R1114, doi:10.1152/ajpregu.00653.2010
Abstract:
The specific transporters involved in maintenance of blood pH homeostasis in cephalopod molluscs have not been identified to date. Using in situ hybridization and immuno histochemical methods, we demonstrate that Na+/K+-ATPase (soNKA), a V-type H+-ATPase (soV-HA), and Na+/HCO3- cotransporter (soNBC) are co-localized in NKA-rich cells in the gills of Sepia officinalis. mRNA expression patterns of these transporters and selected metabolic genes were examined in response to moderately elevated seawater pCO2 (0.16 and 0.35 kPa) over a time-course of six weeks in different ontogenetic stages. The applied CO2 concentrations are relevant for ocean acidification scenarios projected for the coming decades. We determined strong expression changes in late stage embryos and hatchlings, with one to three log2-fold reductions in soNKA, soNBCe, socCAII and COX. In contrast, no hypercapnia induced changes in mRNA expression were observed in juveniles during both short- and long-term exposure. However a transiently increased demand of ion regulatory demand was evident during the initial acclimation reaction to elevated seawater pCO2. Gill Na+/K+-ATPase activity and protein concentration were increased by approximately 15% in during short (2-11 day), but not long term (42 day) exposure. Our findings support the hypothesis that the energy budget of adult cephalopods is not significantly compromised during long-term exposure to moderate environmental hypercapnia. However, the down regulation of ion-regulatory and metabolic genes in late stage embryos, taken together with a significant reduction in somatic growth, indicates that cephalopod early life stages are challenged by elevated seawater pCO2.
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Species *SpeciesHu, Marian Y *
2Comment *CommentHu, Marian Y *
3Experimental treatment *Exp trtmHu, Marian Y *
4Experiment day *Exp daydayHu, Marian Y *
5Salinity *SalHu, Marian Y *
6Salinity, standard deviation *Sal std dev±Hu, Marian Y *
7Temperature, water *Temp°CHu, Marian Y *
8Temperature, standard deviation *T std dev±Hu, Marian Y *
9pH *pHHu, Marian Y *WTW 340i pH-analyzer and WTW SenTix 81-electrode *NBS scale
10pH, standard deviation *pH std dev±Hu, Marian Y *
11Aragonite saturation state *Omega ArgHu, Marian Y *Calculated using CO2SYS *
12Aragonite saturation state, standard deviation *Omega Arg std dev±Hu, Marian Y *
13Carbon dioxide, partial pressure *pCO2PaHu, Marian Y *Calculated using CO2SYS *
14Carbon dioxide, partial pressure, standard deviation *pCO2 std dev±Hu, Marian Y *
15Alkalinity, total *ATµmol/kgHu, Marian Y *Potentiometric open-cell titration *
16Alkalinity, total, standard deviation *AT std dev±Hu, Marian Y *
17Sepia officinalis, mass *S. officinalis mgHu, Marian Y *Precision scale (Sartorius TE64, Sartorius AG, Germany) *
18Sepia officinalis, mass, standard deviation *S. officinalis m std dev±Hu, Marian Y *
19Sepia officinalis, length, mantle *S. officinalis lmmHu, Marian Y *Stereomicroscopy (Leica) *
20Sepia officinalis, length, mantle, standard deviation *S. officinalis l std dev±Hu, Marian Y *
21Sodium/Potassium adenosine triphosphatase gene expression *Na/K-ATPaseHu, Marian Y *see reference(s) *
22Sodium/Potassium adenosine triphosphatase gene expression, standard deviation *Na/K-ATPase std dev±Hu, Marian Y *
23Sodium/bicarbonate cotransporter gene expression *Na/HCO3 cotranspHu, Marian Y *see reference(s) *
24Sodium/bicarbonate cotransporter gene expression, standard deviation *Na/HCO3 cotransp std dev±Hu, Marian Y *
25Sodium driven chlorine/bicarbonate exchanger gene expression *Na-driven Cl/HCO3 exchangerHu, Marian Y *see reference(s) *
26Sodium driven chlorine/bicarbonate exchanger gene expression, standard deviation *Na-driven Cl/HCO3 exchanger std dev±Hu, Marian Y *
27Carbonic anhydrase gene expression *CAHu, Marian Y *see reference(s) *
28Carbonic anhydrase gene expression, standard deviation *CA std dev±Hu, Marian Y *
29Adenosine triphosphate synthase gene expression *ATP-synthHu, Marian Y *see reference(s) *
30Adenosine triphosphate synthase gene expression, standard deviation *ATP-synth std dev±Hu, Marian Y *
31Cytochrome P-450 gene expression *CYP 450Hu, Marian Y *see reference(s) *
32Cytochrome P-450 gene expression, standard deviation *CYP 450 std dev±Hu, Marian Y *
33Cytochrome-c-oxydase gene expression *COXHu, Marian Y *see reference(s) *
34Cytochrome-c-oxydase gene expression, standard deviation *COX std dev±Hu, Marian Y *
35Octopine dehydrogenase gene expression *ODHHu, Marian Y *see reference(s) *
36Octopine dehydrogenase gene expression, standard deviation *ODH std dev±Hu, Marian Y *
37Sodium/Potassium adenosine triphosphatase maximum *Na/K-ATPase maxHu, Marian Y *see reference(s) *
38Sodium/Potassium adenosine triphosphatase maximum, standard deviation *Na/K-ATPase max std dev±Hu, Marian Y *
39Sodium/Potassium adenosine triphosphatase relative protein concentration *Na/K-ATPase protein%Hu, Marian Y *see reference(s) *
40Sodium/Potassium adenosine triphosphatase relative protein concentration, standard deviation *Na/K-ATPase protein std dev±Hu, Marian Y *
41Sodium/Potassium adenosine triphosphatase relative mRNA expression *Na/K-ATPase mRNA%Hu, Marian Y *see reference(s) *
42Sodium/Potassium adenosine triphosphatase relative mRNA expression, standard deviation *Na/K-ATPase mRNA std dev±Hu, Marian Y *
43Sepia officinalis, perivitelline fluid, pH *S. officinalis PVF pHHu, Marian Y *WTW 340i pH-analyzer and WTW SenTix 81-electrode *
44Sepia officinalis, perivitelline fluid, pCO2 *S. officinalis PVF pCO2kPaHu, Marian Y *Calculated using CO2SYS *
45Gene name *GeneHu, Marian Y *
46Sepia officinalis, mRNA gene expression *S. officinalis mRNA expressHu, Marian Y *see reference(s) *
47Sepia officinalis, mRNA gene expression, standard deviation *S. officinalis mRNA express std dev±Hu, Marian Y *
48Sepia officinalis, mRNA gene expression change *S. officinalis mRNA express change%Hu, Marian Y *see reference(s) *
49Carbonate system computation flag *CSC flagNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
50pH *pHNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *Total scale
51Carbon dioxide *CO2µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
52Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
53Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
54Bicarbonate ion *[HCO3]-µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
55Carbonate ion *[CO3]2-µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
56Total carbon *TCµmol/kgHu, Marian Y *Calculated using CO2SYS *
57Aragonite saturation state *Omega ArgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
58Calcite saturation state *Omega CalNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
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