Data Description

Citation:
Roleda, Michael Y; Morris, Jaz N; McGraw, Christina M; Hurd, Catriona L (2011): Seawater carbonate chemistry and giant kelp Macrocystis pyrifera reproduction processes during experiments, 2011. doi:10.1594/PANGAEA.772712
Reference(s):
Roleda, Michael Y; Morris, Jaz N; McGraw, Christina M; Hurd, Catriona L (2011): Ocean acidification and seaweed reproduction: increased CO2 ameliorates the negative effect of lowered pH on meiospore germination in the giant kelp Macrocystis pyrifera (Laminariales, Phaeophyceae). Global Change Biology, in press, in press, doi:10.1111/j.1365-2486.2011.02594.x *
Abstract:
The worldwide effects of ocean acidification (OA) on marine species are a growing concern. In temperate coastal seas, seaweeds are dominant primary producers that create complex habitats and supply energy to higher trophic levels. Studies on OA and macroalgae have focused on calcifying species and adult stages but, critically, they have overlooked the microscopic stages of the reproductive life cycle, which, for other anthropogenic stress e.g. UV-B radiation, are the most susceptible life-history phase. Also, environmental cues and stressors can cause changes in the sex ratio which has implications for the mating system and recruitment success. Here, we report the effects of pH (7.59-8.50) on meiospore germination and sex determination for the giant kelp, Macrocystis pyrifera (Laminariales), in the presence and absence of additional dissolved inorganic carbon (DIC). Lowered pH (7.59-7.60, using HCl-only) caused a significant reduction in germination, while added DIC had the opposite effect, indicating that increased CO2 at lower pH ameliorates physiological stress. This finding also highlights the importance of appropriate manipulation of seawater carbonate chemistry when testing the effects of ocean acidification on photosynthetic organisms. The proportion of male to female gametophytes did not vary significantly between treatments suggesting that pH was not a primary environmental modulator of sex. Relative to the baseline (pH 8.19), gametophytes were 32% larger under moderate OA (pH 7.86) compared to their size (10% increase) under extreme OA (pH 7.61). This study suggests that metabolically-active cells can compensate for the acidification of seawater. This homeostatic function minimises the negative effects of lower pH (high H+ ions) on cellular activity. The 6-9% reduction in germination success under extreme OA suggests that meiospores of M.pyrifera may be resistant to future ocean acidification.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Identification *IDRoleda, Michael Y *
2Experimental treatment *Exp trtmRoleda, Michael Y *
3Salinity *SalRoleda, Michael Y *
4Temperature, water *Temp°CRoleda, Michael Y *
5Total alkalinity *TAµmol/kgRoleda, Michael Y *Closed cell titration *
6Alkalinity, total, standard deviation *Alk std dev±Roleda, Michael Y *
7pH *pHRoleda, Michael Y *pH meter (Orion 720A) *Total scale
8pH, standard deviation *pH std dev±Roleda, Michael Y *
9Total carbon *TCµmol/kgRoleda, Michael Y *calculated using SWCO2 (Hunter, 2007) *
10Carbon, inorganic, total, standard deviation *TIC std dev±Roleda, Michael Y *
11Dihydrogen carbonate *H2CO3µmol/kgRoleda, Michael Y *calculated using SWCO2 (Hunter, 2007) *
12Dihydrogen carbonate, standard deviation *H2CO3 std dev±Roleda, Michael Y *
13Bicarbonate ion concentration *HCO3-µmol/kgRoleda, Michael Y *calculated using SWCO2 (Hunter, 2007) *
14Bicarbonate, standard deviation *HCO3 std dev±Roleda, Michael Y *
15Carbonate ion concentration *CO3**2-µmol/kgRoleda, Michael Y *calculated using SWCO2 (Hunter, 2007) *
16Carbonate ion concentration, standard deviation *CO3**2- std dev±Roleda, Michael Y *
17Partial pressure of Carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmRoleda, Michael Y *calculated using SWCO2 (Hunter, 2007) *
18Carbon dioxyde partial pressure, standard deviation *pCO2 std dev±Roleda, Michael Y *
19Macrocystis pyrifera, germination rate *M. pyrifera germ rate%Roleda, Michael Y *
20Macrocystis pyrifera, germination rate, standard deviation *M. pyrifera germ rate std dev±Roleda, Michael Y *
21Macrocystis pyrifera, sex ratio *M. pyrifera sex ratioRoleda, Michael Y *
22Macrocystis pyrifera, sex ratio, standard deviation *M. pyrifera sex ratio std dev±Roleda, Michael Y *
23Macrocystis pyrifera, gametophyte size *M. pyrifer gametophyterelative unitRoleda, Michael Y *
24Macrocystis pyrifera, gametophyte size, standard deviation *M. pyrifer gametophyte std dev±Roleda, Michael Y *
25Carbonate system computation flag *CSC flagRoleda, Michael Y *calculated using seacarb by Anne-Marin Nisumaa *
26Carbon dioxide *CO2µmol/kgRoleda, Michael Y *calculated using seacarb by Anne-Marin Nisumaa *
27Partial pressure of Carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmRoleda, Michael Y *calculated using seacarb by Anne-Marin Nisumaa *
28Fugacity of Carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmRoleda, Michael Y *calculated using seacarb by Anne-Marin Nisumaa *
29Bicarbonate ion concentration *HCO3-µmol/kgRoleda, Michael Y *calculated using seacarb by Anne-Marin Nisumaa *
30Carbonate ion concentration *CO3**2-µmol/kgRoleda, Michael Y *calculated using seacarb by Anne-Marin Nisumaa *
31Total carbon *TCµmol/kgRoleda, Michael Y *calculated using seacarb by Anne-Marin Nisumaa *
32Aragonite saturation state *SI AragRoleda, Michael Y *calculated using seacarb by Anne-Marin Nisumaa *
33Calcite saturation state *SI CalRoleda, Michael Y *calculated using seacarb by Anne-Marin Nisumaa *
License:
CC-BY Creative Commons Attribution 3.0 Unported
Size:
447 data points

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