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de Bodt, Caroline; Van Oostende, Nicolas; Harlay, Jérôme; Sabbe, Koen; Chou, Lei (2010): Seawater carbonate chemistry, growth rate and Emiliania huxleyi (strain AC481) biological processes during experiments, 2010 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.772696, Supplement to: de Bodt, C et al. (2010): Individual and interacting effects of pCO2 and temperature on Emiliania huxleyi calcification: study of the calcite production, the coccolith morphology and the coccosphere size. Biogeosciences, 7(5), 1401-1412, https://doi.org/10.5194/bg-7-1401-2010

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Abstract:
The impact of ocean acidification and increased water temperature on marine ecosystems, in particular those involving calcifying organisms, has been gradually recognised. We examined the individual and combined effects of increased pCO2 (180 ppmV CO2, 380 ppmV CO2 and 750 ppmV CO2 corresponding to past, present and future CO2 conditions, respectively) and temperature (13 °C and 18 °C) during the exponential growth phase of the coccolithophore E. huxleyi using batch culture experiments. We showed that cellular production rate of Particulate Organic Carbon (POC) increased from the present to the future CO2 treatments at 13 °C. A significant effect of pCO2 and of temperature on calcification was found, manifesting itself in a lower cellular production rate of Particulate Inorganic Carbon (PIC) as well as a lower PIC:POC ratio at future CO2 levels and at 18 °C. Coccosphere-sized particles showed a size reduction with both increasing temperature and CO2concentration. The influence of the different treatments on coccolith morphology was studied by categorizing SEM coccolith micrographs. The number of well-formed coccoliths decreased with increasing pCO2 while temperature did not have a significant impact on coccolith morphology. No interacting effects of pCO2 and temperature were observed on calcite production, coccolith morphology or on coccosphere size. Finally, our results suggest that ocean acidification might have a larger adverse impact on coccolithophorid calcification than surface water warming.
Keyword(s):
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Temperature
Project(s):
European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)
European Project on Ocean Acidification (EPOCA)
Ocean Acidification International Coordination Centre (OA-ICC)
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 Experimental treatmentExp treatde Bodt, Caroline
2 IdentificationIDde Bodt, Caroline
3 SalinitySalde Bodt, Caroline
4 Temperature, waterTemp°Cde Bodt, Caroline
5 Nitrate[NO3]-µmol/lde Bodt, Caroline
6 Phosphate[PO4]3-µmol/lde Bodt, Caroline
7 Radiation, photosynthetically activePARµmol/m2/sde Bodt, Caroline
8 Light:Dark cycleL:Dhh:hhde Bodt, Caroline
9 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmde Bodt, CarolineCalculated using CO2SYST0
10 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmde Bodt, CarolineCalculated using CO2SYSTfin
11 Alkalinity, totalATµmol/kgde Bodt, CarolineAlkalinity, Gran titration (Gran, 1950)T0
12 Alkalinity, totalATµmol/kgde Bodt, CarolineAlkalinity, Gran titration (Gran, 1950)Tfin
13 pHpHde Bodt, CarolinepH meter (Metrohm electrodes)T0; Total scale
14 pHpHde Bodt, CarolinepH meter (Metrohm electrodes)Tfin; Total scale
15 Carbon, inorganic, dissolvedDICµmol/kgde Bodt, CarolineCalculated using CO2SYST0
16 Carbon, inorganic, dissolvedDICµmol/kgde Bodt, CarolineCalculated using CO2SYSTfin
17 Carbon dioxideCO2µmol/kgde Bodt, CarolineCalculated using CO2SYST0
18 Carbon dioxideCO2µmol/kgde Bodt, CarolineCalculated using CO2SYSTfin
19 Bicarbonate ion[HCO3]-µmol/kgde Bodt, CarolineCalculated using CO2SYST0
20 Bicarbonate ion[HCO3]-µmol/kgde Bodt, CarolineCalculated using CO2SYSTfin
21 Calcite saturation stateOmega Calde Bodt, CarolineCalculated using CO2SYST0
22 Calcite saturation stateOmega Calde Bodt, CarolineCalculated using CO2SYSTfin
23 SpeciesSpeciesde Bodt, Caroline
24 Emiliania huxleyiE. huxleyi#/lde Bodt, CarolineHaemocytometer counting
25 Chlorophyll a per cellChl a/cellpg/#de Bodt, CarolineMeasured
26 Carbon, organic, particulatePOCµmol/lde Bodt, CarolineElement analyser, Fisons NA 1500 N
27 Carbon, inorganic, particulatePICµmol/lde Bodt, CarolineCalculated
28 Growth rateµ#/dayde Bodt, CarolineCalculated
29 Particulate inorganic carbon production per cellPIC prod/cellµmol/#/dayde Bodt, CarolineCalculated
30 Particulate organic carbon production per cellPOC prod/cellµmol/#/dayde Bodt, CarolineCalculated
31 Particulate inorganic carbon/particulate organic carbon ratioPIC/POCde Bodt, Caroline
32 Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
33 Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)T0
34 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)T0
35 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)T0
36 Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)T0
37 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)T0
38 Carbon, inorganic, dissolvedDICµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)T0
39 Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)T0
40 Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)T0
41 Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Tfin
42 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Tfin
43 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Tfin
44 Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Tfin
45 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Tfin
46 Carbon, inorganic, dissolvedDICµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Tfin
47 Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Tfin
48 Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Tfin
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
480 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Exp treat		2 
ID		3 
Sal		4 
Temp [°C]		5 
[NO3]- [µmol/l]		6 
[PO4]3- [µmol/l]		7 
PAR [µmol/m2/s]		8 
L:D [hh:hh]		9 
pCO2water_SST_wet [µatm]
(T0, Calculated using CO2SYS)		10 
pCO2water_SST_wet [µatm]
(Tfin, Calculated using CO2SYS)		11 
AT [µmol/kg]
(T0, Alkalinity, Gran titratio...)		12 
AT [µmol/kg]
(Tfin, Alkalinity, Gran titrat...)		13 
pH
(T0; Total scale, pH meter (Me...)		14 
pH
(Tfin; Total scale, pH meter (...)		15 
DIC [µmol/kg]
(T0, Calculated using CO2SYS)		16 
DIC [µmol/kg]
(Tfin, Calculated using CO2SYS)		17 
CO2 [µmol/kg]
(T0, Calculated using CO2SYS)		18 
CO2 [µmol/kg]
(Tfin, Calculated using CO2SYS)		19 
[HCO3]- [µmol/kg]
(T0, Calculated using CO2SYS)		20 
[HCO3]- [µmol/kg]
(Tfin, Calculated using CO2SYS)		21 
Omega Cal
(T0, Calculated using CO2SYS)		22 
Omega Cal
(Tfin, Calculated using CO2SYS)		23 
Species		24 
E. huxleyi [#/l]
(Haemocytometer counting)		25 
Chl a/cell [pg/#]
(Measured)		26 
POC [µmol/l]
(Element analyser, Fisons NA 1...)		27 
PIC [µmol/l]
(Calculated)		28 
µ [#/day]
(Calculated)		29 
PIC prod/cell [µmol/#/day]
(Calculated)		30 
POC prod/cell [µmol/#/day]
(Calculated)		31 
PIC/POC		32 
CSC flag
(Calculated using seacarb afte...)		33 
CO2 [µmol/kg]
(T0, Calculated using seacarb ...)		34 
pCO2water_SST_wet [µatm]
(T0, Calculated using seacarb ...)		35 
fCO2water_SST_wet [µatm]
(T0, Calculated using seacarb ...)		36 
[HCO3]- [µmol/kg]
(T0, Calculated using seacarb ...)		37 
[CO3]2- [µmol/kg]
(T0, Calculated using seacarb ...)		38 
DIC [µmol/kg]
(T0, Calculated using seacarb ...)		39 
Omega Arg
(T0, Calculated using seacarb ...)		40 
Omega Cal
(T0, Calculated using seacarb ...)		41 
CO2 [µmol/kg]
(Tfin, Calculated using seacar...)		42 
pCO2water_SST_wet [µatm]
(Tfin, Calculated using seacar...)		43 
fCO2water_SST_wet [µatm]
(Tfin, Calculated using seacar...)		44 
[HCO3]- [µmol/kg]
(Tfin, Calculated using seacar...)		45 
[CO3]2- [µmol/kg]
(Tfin, Calculated using seacar...)		46 
DIC [µmol/kg]
(Tfin, Calculated using seacar...)		47 
Omega Arg
(Tfin, Calculated using seacar...)		48 
Omega Cal
(Tfin, Calculated using seacar...)
Low CO2Replicate 135.61332115014:10179.8246.92232.71568.38.38.11817.71355.5287.1132.71524.51214.46.83.2Emiliania huxleyi (strain AC481)2340000000.22154.2383.30.130.000000150.000000220.68087.54190.65189.951655.15230.281892.973.505.469.13230.87230.021264.65111.011384.801.692.63
Low CO2Replicate 235.61332115014:10221.4232.22230.51567.28.28.11862.51345.4256.3137.61598.61199.96.13.3Emiliania huxleyi (strain AC481)2340000000.12135.8331.70.120.000000180.000000092.130810.02253.24252.311746.35192.991949.372.934.589.13230.70229.851263.71110.931383.771.682.63
Present CO2Replicate 135.61332115014:10387.7377.82252.41652.08.17.91996.11486.8185.1109.51797.71364.54.42.6Emiliania huxleyi (strain AC481)2220000000.07142.5311.70.100.000000140.000000062.190813.34337.10335.871846.58162.102022.012.463.8416.45415.93414.411437.5679.621533.641.211.89
Present CO2Replicate 235.61332115014:10388.5493.92246.01347.38.17.81991.11254.4184.062.11793.91175.54.41.5Emiliania huxleyi (strain AC481)2560000000.08174.2479.20.100.000000150.000000072.250813.30336.11334.881841.14161.622016.052.453.8317.25436.00434.401197.0052.661266.910.801.25
Future CO2Replicate 135.61332115014:10768.4837.92323.01343.17.87.62177.61300.8119.340.22032.01232.02.81.0Emiliania huxleyi (strain AC481)3090000000.14257.5377.50.110.000000090.000000120.770830.17762.65759.862093.7992.122216.071.402.1828.42718.46715.831244.5434.551307.520.520.82
Future CO2Replicate 235.61332115014:10715.8821.22326.71339.87.87.62169.31296.0126.440.72018.51227.33.01.0Emiliania huxleyi (strain AC481)2880000000.12240.0453.30.110.000000120.000000130.940830.22763.89761.092097.1992.272219.671.402.1928.35716.66714.041241.4234.461304.230.520.82
Present CO2Replicate 135.61832115014:10490.6473.82069.2991.97.97.71883.1935.1135.636.51730.8882.53.20.9Emiliania huxleyi (strain AC481)6840000000.05430.0474.20.150.000000100.000000160.630818.02529.25527.431768.74118.661905.431.822.8214.15415.33413.91875.8037.07927.010.570.88
Present CO2Replicate 235.61832115014:10437.6473.22045.01090.98.07.61843.4887.1144.033.01684.6838.03.40.8Emiliania huxleyi (strain AC481)5200000000.05441.7620.80.120.000000100.000000110.860813.63400.21398.841683.81142.211839.652.193.3820.14591.26589.23990.3433.301043.780.510.79
Future CO2Replicate 135.61832115014:10784.1745.32101.31255.87.87.61982.81210.297.839.11858.31145.72.30.9Emiliania huxleyi (strain AC481)5350000000.03375.0486.70.110.000000080.000000090.880823.76697.75695.351852.2898.701974.751.522.3523.28683.51681.161144.8538.491206.620.590.91
Future CO2Replicate 235.61832115014:10795.6822.82152.91265.47.87.52031.21227.4101.136.51903.01162.92.40.9Emiliania huxleyi (strain AC481)4000000000.03460.0576.70.090.000000060.000000130.470824.36715.32712.861898.91101.192024.461.562.4030.08883.32880.291175.2431.391236.710.480.75