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Witt, Verena; Wild, Christian; Anthony, Kenneth R N; Diaz-Pulido, Guillermo; Uthicke, Sven (2011): Seawater carbonate chemistry, nitrogen concentration and macro community analyse, 2011 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.770491, Supplement to: Witt, V et al. (2011): Effects of ocean acidification on microbial community composition of, and oxygen fluxes through, biofilms from the Great Barrier Reef. Environmental Microbiology, 13(11), 2976-2989, https://doi.org/10.1111/j.1462-2920.2011.02571.x

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Abstract:
Rising anthropogenic CO2 emissions acidify the oceans, and cause changes to seawater carbon chemistry. Bacterial biofilm communities reflect environmental disturbances and may rapidly respond to ocean acidification. This study investigates community composition and activity responses to experimental ocean acidification in biofilms from the Australian Great Barrier Reef. Natural biofilms grown on glass slides were exposed for 11 d to four controlled pCO2 concentrations representing the following scenarios: A) pre-industrial (~300 ppm), B) present-day (~400 ppm), C) mid century (~560 ppm) and D) late century (~1140 ppm). Terminal restriction fragment length polymorphism and clone library analyses of 16S rRNA genes revealed CO2-correlated bacterial community shifts between treatments A, B and D. Observed bacterial community shifts were driven by decreases in the relative abundance of Alphaproteobacteria and increases of Flavobacteriales (Bacteroidetes) at increased CO2 concentrations, indicating pH sensitivity of specific bacterial groups. Elevated pCO2 (C + D) shifted biofilm algal communities and significantly increased C and N contents, yet O2 fluxes, measured using in light and dark incubations, remained unchanged. Our findings suggest that bacterial biofilm communities rapidly adapt and reorganize in response to high pCO2 to maintain activity such as oxygen production.
Keyword(s):
Benthos; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Community composition and diversity; Entire community; Laboratory experiment; Rocky-shore community; South Pacific; Temperate
Project(s):
European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)
European Project on Ocean Acidification (EPOCA)
Ocean Acidification International Coordination Centre (OA-ICC)
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 Experimental treatmentExp treatWitt, Verena
2 SalinitySalWitt, Verena
3 Temperature, waterTemp°CWitt, VerenaTemperature logger, Onset, UA-001
4 pHpHWitt, VerenapH meter (HQ10-HQ20 Meters, Hach, USA)NBS scale
5 pH, standard errorpH std e±Witt, Verena
6 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmWitt, VerenaCalculated using CO2SYS
7 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard errorpCO2water_SST_wet std e±Witt, Verena
8 Alkalinity, totalATµmol/kgWitt, VerenaPotentiometric open-cell titration
9 Alkalinity, total, standard errorAT std e±Witt, Verena
10 Carbon, inorganic, dissolvedDICµmol/kgWitt, VerenaCalculated using CO2SYS
11 Carbon, inorganic, dissolved, standard errorDIC std e±Witt, Verena
12 Aragonite saturation stateOmega ArgWitt, VerenaCalculated using CO2SYS
13 Aragonite saturation state, standard deviationOmega Arg std dev±Witt, Verena
14 Carbon, inorganic, totalTICg/m2Witt, VerenaElement analyser, THERMO NA 2500
15 Carbon, inorganic, total, standard deviationTIC std dev±Witt, Verena
16 Carbon, organic, totalTOCg/m2Witt, VerenaTOC analyzer (Shimadzu)
17 Carbon, organic, total, standard deviationTOC std dev±Witt, Verena
18 Nitrogen, totalTNg/m2Witt, VerenaElement analyser, THERMO NA 2500
19 Nitrogen, total, standard deviationTN std dev±Witt, Verena
20 Nitrogen, inorganicN inorg%Witt, Verena
21 Carbon/Nitrogen ratio, standard deviationC/N std dev±Witt, Verena
22 ReplicatesRepl#Witt, Verena
23 Experiment dayExp daydayWitt, Verena
24 DiatomsDiatoms#Witt, Verena
25 AlgaeAlg#Witt, VerenaFilamentous green
26 AlgaeAlg#Witt, VerenaGreen
27 AlgaeAlg#Witt, VerenaFilamentous red
28 AlgaeAlg#Witt, VerenaCalcareous red
29 AlgaeAlg#Witt, VerenaFleshy red
30 Carbon, inorganic, dissolvedDICµmol/kgWitt, VerenaCalculated using CO2SYS
31 Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
32 pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
33 Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
34 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
35 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
36 Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
37 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
38 Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
39 Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
156 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Exp treat		2 
Sal		3 
Temp [°C]
(Temperature logger, Onset, UA...)		4 
pH
(NBS scale, pH meter (HQ10-HQ2...)		5 
pH std e [±]		6 
pCO2water_SST_wet [µatm]
(Calculated using CO2SYS)		7 
pCO2water_SST_wet std e [±]		8 
AT [µmol/kg]
(Potentiometric open-cell titr...)		9 
AT std e [±]		10 
DIC [µmol/kg]
(Calculated using CO2SYS)		11 
DIC std e [±]		12 
Omega Arg
(Calculated using CO2SYS)		13 
Omega Arg std dev [±]		14 
TIC [g/m2]
(Element analyser, THERMO NA 2500)		15 
TIC std dev [±]		16 
TOC [g/m2]
(TOC analyzer (Shimadzu))		17 
TOC std dev [±]		18 
TN [g/m2]
(Element analyser, THERMO NA 2500)		19 
TN std dev [±]		20 
N inorg [%]		21 
C/N std dev [±]		22 
Repl [#]		23 
Exp day [day]		24 
Diatoms [#]		25 
Alg [#]
(Filamentous green)		26 
Alg [#]
(Green)		27 
Alg [#]
(Filamentous red)		28 
Alg [#]
(Calcareous red)		29 
Alg [#]
(Fleshy red)		30 
DIC [µmol/kg]
(Calculated using CO2SYS)		31 
CSC flag
(Calculated using seacarb afte...)		32 
pH
(Total scale, Calculated using...)		33 
CO2 [µmol/kg]
(Calculated using seacarb afte...)		34 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		35 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		36 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)		37 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)		38 
Omega Arg
(Calculated using seacarb afte...)		39 
Omega Cal
(Calculated using seacarb afte...)
Pre-industrial33248.120.02305112193131882163.370.060.840.261.370.190.180.037.7673.5886112315186661954.6267.9813.31459.39457.941769.11172.192.764.21
Today33248.020.02402202170271918292.780.071.282.381.320.090.230.657.1721.8166112415186661980.8267.8817.26595.83593.941822.63140.912.263.44
Projected mid century under A1Fi scenario33247.850.01564122208222012172.260.071.572.951.570.500.200.967.9872.225611241592112086.6267.7127.41946.18943.181956.90102.291.642.50
Projected late century under A1Fi scenario33247.630.021140522212202123241.320.041.894.761.750.220.250.868.2450.556611221520002160.4267.5145.771580.151575.152047.5767.061.071.64