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Witt, Verena; Wild, Christian; Anthony, Kenneth R N; Diaz-Pulido, Guillermo; Uthicke, Sven (2011): Seawater carbonate chemistry, nitrogen concentration and macro community analyse, 2011 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.770491, Supplement to: Witt, V et al. (2011): Effects of ocean acidification on microbial community composition of, and oxygen fluxes through, biofilms from the Great Barrier Reef. Environmental Microbiology, 13(11), 2976-2989, https://doi.org/10.1111/j.1462-2920.2011.02571.x

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Abstract:
Rising anthropogenic CO2 emissions acidify the oceans, and cause changes to seawater carbon chemistry. Bacterial biofilm communities reflect environmental disturbances and may rapidly respond to ocean acidification. This study investigates community composition and activity responses to experimental ocean acidification in biofilms from the Australian Great Barrier Reef. Natural biofilms grown on glass slides were exposed for 11 d to four controlled pCO2 concentrations representing the following scenarios: A) pre-industrial (~300 ppm), B) present-day (~400 ppm), C) mid century (~560 ppm) and D) late century (~1140 ppm). Terminal restriction fragment length polymorphism and clone library analyses of 16S rRNA genes revealed CO2-correlated bacterial community shifts between treatments A, B and D. Observed bacterial community shifts were driven by decreases in the relative abundance of Alphaproteobacteria and increases of Flavobacteriales (Bacteroidetes) at increased CO2 concentrations, indicating pH sensitivity of specific bacterial groups. Elevated pCO2 (C + D) shifted biofilm algal communities and significantly increased C and N contents, yet O2 fluxes, measured using in light and dark incubations, remained unchanged. Our findings suggest that bacterial biofilm communities rapidly adapt and reorganize in response to high pCO2 to maintain activity such as oxygen production.
Keyword(s):
Benthos; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Community composition and diversity; Entire community; Laboratory experiment; Rocky-shore community; South Pacific; Temperate
Project(s):
European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)
European Project on Ocean Acidification (EPOCA)
Ocean Acidification International Coordination Centre (OA-ICC)
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Experimental treatmentExp treatWitt, Verena
2SalinitySalWitt, Verena
3Temperature, waterTemp°CWitt, VerenaTemperature logger, Onset, UA-001
4pHpHWitt, VerenapH meter (HQ10-HQ20 Meters, Hach, USA)NBS scale
5pH, standard errorpH std e±Witt, Verena
6Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmWitt, VerenaCalculated using CO2SYS
7Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard errorpCO2water_SST_wet std e±Witt, Verena
8Alkalinity, totalATµmol/kgWitt, VerenaPotentiometric open-cell titration
9Alkalinity, total, standard errorAT std e±Witt, Verena
10Carbon, inorganic, dissolvedDICµmol/kgWitt, VerenaCalculated using CO2SYS
11Carbon, inorganic, dissolved, standard errorDIC std e±Witt, Verena
12Aragonite saturation stateOmega ArgWitt, VerenaCalculated using CO2SYS
13Aragonite saturation state, standard deviationOmega Arg std dev±Witt, Verena
14Carbon, inorganic, totalTICg/m2Witt, VerenaElement analyser, THERMO NA 2500
15Carbon, inorganic, total, standard deviationTIC std dev±Witt, Verena
16Carbon, organic, totalTOCg/m2Witt, VerenaTOC analyzer (Shimadzu)
17Carbon, organic, total, standard deviationTOC std dev±Witt, Verena
18Nitrogen, totalTNg/m2Witt, VerenaElement analyser, THERMO NA 2500
19Nitrogen, total, standard deviationTN std dev±Witt, Verena
20Nitrogen, inorganicN inorg%Witt, Verena
21Carbon/Nitrogen ratio, standard deviationC/N std dev±Witt, Verena
22ReplicatesRepl#Witt, Verena
23Experiment dayExp daydayWitt, Verena
24DiatomsDiatoms#Witt, Verena
25AlgaeAlg#Witt, VerenaFilamentous green
26AlgaeAlg#Witt, VerenaGreen
27AlgaeAlg#Witt, VerenaFilamentous red
28AlgaeAlg#Witt, VerenaCalcareous red
29AlgaeAlg#Witt, VerenaFleshy red
30Carbon, inorganic, dissolvedDICµmol/kgWitt, VerenaCalculated using CO2SYS
31Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
32pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
33Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
34Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
35Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
36Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
37Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
38Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
39Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
156 data points

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