Ma, H et al. (2011): Data files of figures 1, 2 and 3. doi:10.1594/PANGAEA.769888, Supplement to:Ma, Haiyan; Krock, Bernd; Tillmann, Urban; Cembella, Allan (2010): Towards characterization of lytic compound(s) produced by Alexandrium tamarense. Proceedings of the 13th International Conference on Harmful Algae, Ed. Ho, K. et al., International Society for the Study of Harmful Algae and Intergovernmental Oceanographic Commission of UNESCO, Hong Kong, 2008, China, 142-146, hdl:10013/epic.36232.d001
We investigated optimal conditions for characterization of bioactivity of lytic compound(s) excreted by Alexandrium tamarense based on a cell-bioassay system. Allelochemical response of the cryptophyte Rhodomonas salina indicated the presence oflytic compound(s) in a reliable and reproducible way and allows for quantification of this lytic effect. The parameters tested were the incubation time of putatively lytic extracts or fractions with the target organism R. salina, different techniques for cell harvest from A. tamarense cultures and the optimal harvest time. A three hour incubation time was found to be optimal to yield a rapid response while accurately estimating effective concentration (ECso) values. Harvest of A. tamarense cultures by filtration resulted in loss of lytic activity in most cases and centrifugation was most efficient in terms of recovery of lytic activity. Maximum yield of extracellular lytic activity of A. tamarense cultures was achieved in the stationary phase. Such optimized bioassay guided fractionation techniques are a valuable asset in the isolation and eventual stmctural elucidation of the unknown lytic substances.