Data Description

  RIS BibTeX
Citation:
Lannig, G et al. (2011): Seawater carbonate chemistry and biological processes of mussel Crassostrea gigas during experiments, 2011. doi:10.1594/PANGAEA.761915,
Supplement to: Lannig, Gisela; Eilers, Silke; Pörtner, Hans-Otto; Sokolova, Inna A; Bock, Christian (2010): Impact of ocean acidification on energy metabolism of oyster, Crassostrea gigas-Changes in metabolic pathways and thermal response. Marine Drugs, 8(8), 2318-2339, doi:10.3390/md8082318
Abstract:
Climate change with increasing temperature and ocean acidification (OA) poses risks for marine ecosystems. According to Pörtner and Farrell [1], synergistic effects of elevated temperature and CO2-induced OA on energy metabolism will narrow the thermal tolerance window of marine ectothermal animals. To test this hypothesis, we investigated the effect of an acute temperature rise on energy metabolism of the oyster, Crassostrea gigas chronically exposed to elevated CO2 levels (partial pressure of CO2 in the seawater ~0.15 kPa, seawater pH ~ 7.7). Within one month of incubation at elevated PCO2 and 15 °C hemolymph pH fell (pHe = 7.1 ± 0.2 (CO2-group) vs. 7.6 ± 0.1 (control)) and PeCO2 values in hemolymph increased (0.5 ± 0.2 kPa (CO2-group) vs. 0.2 ± 0.04 kPa (control)). Slightly but significantly elevated bicarbonate concentrations in the hemolymph of CO2-incubated oysters ([HCO-3]e = 1.8 ± 0.3 mM (CO2-group) vs. 1.3 ± 0.1 mM (control)) indicate only minimal regulation of extracellular acid-base status. At the acclimation temperature of 15 °C the OA-induced decrease in pHe did not lead to metabolic depression in oysters as standard metabolism rates (SMR) of CO2-exposed oysters were similar to controls. Upon acute warming SMR rose in both groups, but displayed a stronger increase in the CO2-incubated group. Investigation in isolated gill cells revealed a similar temperature-dependence of respiration between groups. Furthermore, the fraction of cellular energy demand for ion regulation via Na+/K+-ATPase was not affected by chronic hypercapnia or temperature. Metabolic profiling using 1H-NMR spectroscopy revealed substantial changes in some tissues following OA exposure at 15 °C. In mantle tissue alanine and ATP levels decreased significantly whereas an increase in succinate levels was observed in gill tissue. These findings suggest shifts in metabolic pathways following OA-exposure. Our study confirms that OA affects energy metabolism in oysters and suggests that climate change may affect populations of sessile coastal invertebrates such as mollusks
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Experimental treatment *Exp trtmLannig, Gisela *
2Salinity *SalLannig, Gisela *
3Salinity, standard deviation *Sal std devLannig, Gisela *
4Replicates *N#Lannig, Gisela *
5Temperature, water *Temp░CLannig, Gisela *
6pH *pHLannig, Gisela *Measured *NBS scale
7pH, standard deviation *pH std devLannig, Gisela *NBS scale
8Carbon dioxide, partial pressure *pCO2PaLannig, Gisela *Calculated using CO2SYS *
9Carbon dioxide, partial pressure, standard deviation *pCO2 std devLannig, Gisela *
10Bicarbonate ion *[HCO3]-Ámol/kgLannig, Gisela *Calculated using CO2SYS *
11Bicarbonate ion, standard deviation *[HCO3]- std devLannig, Gisela *
12Calcite saturation state *Omega CalLannig, Gisela *Calculated using CO2SYS *
13Calcite saturation state, standard deviation *Omega Cal std devLannig, Gisela *
14Aragonite saturation state *Omega ArgLannig, Gisela *Calculated using CO2SYS *
15Aragonite saturation state, standard deviation *Omega Arg std devLannig, Gisela *
16Crassostrea gigas, haemolymph, pH *C. gigas pH (ha)Lannig, Gisela *
17Crassostrea gigas, haemolymph, partial pressure of carbon dioxide *C. gigas pCO2 (ha)kPaLannig, Gisela *Gas chromatography *
18Crassostrea gigas, haemolymph, partial pressure of oxygen *C. gigas pO2 (ha)kPaLannig, Gisela *Gas chromatography *
19Crassostrea gigas, haemolymph, dissolved inorganic carbon *C. gigas DIC (ha)mmol/lLannig, Gisela *Ion chromatography *
20Crassostrea gigas, haemolymph, bicarbonate ion *C. gigas [HCO3]- (ha)mmol/lLannig, Gisela *Ion chromatography *
21Crassostrea gigas, haemolymph, calcium ion *C. gigas Ca2+ (ha)mmol/lLannig, Gisela *Ion chromatography *
22Crassostrea gigas, haemolymph, sodium ion *C. gigas Na+ (ha)mmol/lLannig, Gisela *Ion chromatography *
23Crassostrea gigas, haemolymph, potassium ion *C. gigas K+ (ha)mmol/lLannig, Gisela *Ion chromatography *
24Crassostrea gigas, alanine, wet tissue mass *C. gigas alanineÁmol/gLannig, Gisela *TopSpin 2.5 (Bruker Biospin GmbH, Germany) *Muscle
25Crassostrea gigas, succinate, wet tissue mass *C. gigas succinateÁmol/gLannig, Gisela *TopSpin 2.5 (Bruker Biospin GmbH, Germany) *Muscle
26Crassostrea gigas, glycogen, wet tissue mass *C. gigas glycogenÁmol/gLannig, Gisela *TopSpin 2.5 (Bruker Biospin GmbH, Germany) *Muscle
27Crassostrea gigas, ATP, wet tissue mass *C. gigas ATPÁmol/gLannig, Gisela *TopSpin 2.5 (Bruker Biospin GmbH, Germany) *Muscle
28Crassostrea gigas, alanine, wet tissue mass *C. gigas alanineÁmol/gLannig, Gisela *TopSpin 2.5 (Bruker Biospin GmbH, Germany) *Mantle
29Crassostrea gigas, succinate, wet tissue mass *C. gigas succinateÁmol/gLannig, Gisela *TopSpin 2.5 (Bruker Biospin GmbH, Germany) *Mantle
30Crassostrea gigas, glycogen, wet tissue mass *C. gigas glycogenÁmol/gLannig, Gisela *TopSpin 2.5 (Bruker Biospin GmbH, Germany) *Mantle
31Crassostrea gigas, ATP, wet tissue mass *C. gigas ATPÁmol/gLannig, Gisela *TopSpin 2.5 (Bruker Biospin GmbH, Germany) *Mantle
32Crassostrea gigas, alanine, wet tissue mass *C. gigas alanineÁmol/gLannig, Gisela *TopSpin 2.5 (Bruker Biospin GmbH, Germany) *Gills
33Crassostrea gigas, succinate, wet tissue mass *C. gigas succinateÁmol/gLannig, Gisela *TopSpin 2.5 (Bruker Biospin GmbH, Germany) *Gills
34Crassostrea gigas, glycogen, wet tissue mass *C. gigas glycogenÁmol/gLannig, Gisela *TopSpin 2.5 (Bruker Biospin GmbH, Germany) *Gills
35Crassostrea gigas, ATP, wet tissue mass *C. gigas ATPÁmol/gLannig, Gisela *TopSpin 2.5 (Bruker Biospin GmbH, Germany) *Gills
36Condition index *CILannig, Gisela *
37Metabolic rate of oxygen, standard, normalized *Met rate O2 normÁmol/h/gLannig, Gisela *
38Crassostrea gigas, respiration rate, oxygen, per cell *C. gigas resp O2nmol/min/106#Lannig, Gisela *
39Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetÁatmLannig, Gisela *
40Carbonate system computation flag *CSC flagNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
41pH *pHNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *Total scale
42Carbon dioxide *CO2Ámol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
43Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetÁatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
44Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetÁatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
45Bicarbonate ion *[HCO3]-Ámol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
46Carbonate ion *[CO3]2-Ámol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
47Total carbon *TCÁmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
48Alkalinity, total *ATÁmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
49Aragonite saturation state *Omega ArgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
50Calcite saturation state *Omega CalNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
License:
Size:
2179 data points

Download Data

Download dataset as tab-delimited text (use the following character encoding: )

View dataset as HTML (shows only first 2000 rows)