Range, P; ChÌcharo, M A; Ben-Hamadou, R; Pilò, D; Matias, D; Joaquim, S; Oliveira, A P; ChÌcharo, L (2011): Seawater carbonate chemistry and Ruditapes decussatus biological processes during experiments, 2011 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.758702, Supplement to: Range, P et al. (2011): Calcification, growth and mortality of juvenile clams Ruditapes decussatus under increased pCO2 and reduced pH: Variable responses to ocean acidification at local scales? Journal of Experimental Marine Biology and Ecology, 396(2), 177-184, https://doi.org/10.1016/j.jembe.2010.10.020
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Abstract:
We investigated the effects of ocean acidification on juvenile clams Ruditapes decussatus (average shell length 10.24 mm) in a controlled CO2 perturbation experiment. The carbonate chemistry of seawater was manipulated by diffusing pure CO2, to attain two reduced pH levels (by -0.4 and -0.7 pH units), which were compared to unmanipulated seawater. After 75 days we found no differences among pH treatments in terms of net calcification, size or weight of the clams. The naturally elevated total alkalinity of local seawater probably contributed to buffer the effects of increased pCO2 and reduced pH. Marine organisms may, therefore, show diverse responses to ocean acidification at local scales, particularly in coastal, estuarine and transitional waters, where the physical-chemical characteristics of seawater are most variable. Mortality was significantly reduced in the acidified treatments. This trend was probably related to the occurrence of spontaneous spawning events in the control and intermediate acidification treatments. Spawning, which was unexpected due to the small size of the clams, was not observed for the pH -0.7 treatment, suggesting that the increased survival under acidified conditions may have been associated with a delay in the reproductive cycle of the clams. Future research about the impacts of ocean acidification on marine biodiversity should be extended to other types of biological and ecological processes, apart from biological calcification.
Keyword(s):
Project(s):
Funding:
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
750 data points
Data
1 Date | 2 Sample ID | 3 Exp treat | 4 pH (Total scale, Aqua Medic elect...) | 5 Sal (Aqua Medic electrodes and the...) | 6 Temp [°C] (Aqua Medic electrodes and the...) | 7 AT [µmol/kg] | 8 DIC [µmol/kg] (see reference(s)) | 9 CO2 [µmol/kg] (see reference(s)) | 10 pCO2water_SST_wet [µatm] (see reference(s)) | 11 Omega Cal (see reference(s)) | 12 Omega Arg (see reference(s)) | 13 R. decussatus Wshell [g] (Measured) | 14 R. decussatus Wdry flesh [g] (Measured) | 15 CI | 16 Mortality (Mortality based on Taylor (1958)) | 17 R. decussatus sl increase [%] | 18 R. decussatus width increase [%] | 19 R. decussatus Wlive increase [%] | 20 CSC flag (Calculated using seacarb afte...) | 21 CO2 [µmol/kg] (Calculated using seacarb afte...) | 22 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 23 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 24 [HCO3]- [µmol/kg] (Calculated using seacarb afte...) | 25 [CO3]2- [µmol/kg] (Calculated using seacarb afte...) | 26 DIC [µmol/kg] (Calculated using seacarb afte...) | 27 Omega Arg (Calculated using seacarb afte...) | 28 Omega Cal (Calculated using seacarb afte...) |
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2009-07-20 | Tank 1 Clam | Low pH | 7.84 | 30.04 | 22.90 | 3889.6 | 3727.3 | 50.2 | 1698.4 | 4.3 | 2.8 | 8 | 37.95 | 1237.53 | 1233.53 | 3418.81 | 209.97 | 3666.7 | 3.39 | 5.23 | |||||||
2009-07-20 | Tank 2 Clam | Very low pH | 7.57 | 30.43 | 22.57 | ||||||||||||||||||||||
2009-07-20 | Tank 3 Clam | Control | 8.13 | 30.42 | 22.50 | 3917.4 | 3551.1 | 23.3 | 804.8 | 7.9 | 5.1 | 8 | 17.67 | 571.35 | 569.50 | 3091.55 | 368.30 | 3477.5 | 5.92 | 9.14 | |||||||
2009-07-20 | Tank 4 Algae | Low pH | 7.83 | 30.05 | 22.86 | 3916.4 | 3750.3 | 50.6 | 1768.6 | 4.4 | 2.9 | 8 | 39.25 | 1278.51 | 1274.37 | 3452.66 | 206.97 | 3698.9 | 3.34 | 5.15 | |||||||
2009-07-20 | Tank 5 Clam | Very low pH | 7.57 | 30.02 | 22.87 | ||||||||||||||||||||||
2009-07-20 | Tank 6 Algae | Control | 8.21 | 30.06 | 22.83 | ||||||||||||||||||||||
2009-07-20 | Tank 7 Clam | Low pH | 7.79 | 30.03 | 22.87 | ||||||||||||||||||||||
2009-07-20 | Tank 8 Algae | Very low pH | 7.44 | 30.43 | 22.52 | ||||||||||||||||||||||
2009-07-20 | Tank 9 Clam | Control | 8.17 | 30.10 | 22.85 | ||||||||||||||||||||||
2009-07-20 | Tank 10 Clam | Low pH | 7.83 | 30.05 | 21.91 | ||||||||||||||||||||||
2009-07-20 | Tank 11 Algae | Very low pH | 7.51 | 29.70 | 23.10 | ||||||||||||||||||||||
2009-07-20 | Tank 12 Clam | Control | 8.19 | 29.36 | 23.09 | ||||||||||||||||||||||
2009-07-20 | Tank 13 blank | Low pH | 7.85 | 30.13 | 22.80 | 3909.3 | 3733.7 | 48.2 | 1675.4 | 4.5 | 3.0 | 8 | 37.22 | 1210.98 | 1207.07 | 3427.19 | 215.08 | 3679.5 | 3.46 | 5.35 | |||||||
2009-07-20 | Tank 14 blank | Very low pH | 7.67 | 30.04 | 22.80 | 3907.4 | 3830.3 | 75.8 | 2624.8 | 3.1 | 2.0 | 8 | 58.83 | 1913.31 | 1907.12 | 3575.82 | 147.93 | 3782.6 | 2.38 | 3.68 | |||||||
2009-07-20 | Tank 15 Algae | Control | 8.22 | 30.06 | 22.85 | 3901.5 | 3460.9 | 18.1 | 625.6 | 9.2 | 6.0 | 8 | 13.59 | 442.57 | 441.14 | 2934.15 | 431.70 | 3379.4 | 6.96 | 10.75 | |||||||
2009-07-20 | Tank 17 blank | Very low pH | 7.53 | 30.01 | 22.85 | ||||||||||||||||||||||
2009-07-20 | Tank 18 blank | Control | 8.25 | 30.01 | 23.13 | 3910.1 | 3443.5 | 16.7 | 576.5 | 9.7 | 6.4 | 8 | 12.40 | 406.63 | 405.32 | 2882.87 | 458.64 | 3353.9 | 7.40 | 11.43 | |||||||
2009-07-20 | Tank 19 Algae | Low pH | 8.00 | 29.95 | 22.82 | ||||||||||||||||||||||
2009-07-20 | Tank 20 Clam | Very low pH | 7.46 | 30.08 | 22.82 | 3901.6 | 3934.2 | 127.5 | 4345.2 | 1.9 | 1.2 | 8 | 98.38 | 3201.97 | 3191.62 | 3690.17 | 94.29 | 3882.8 | 1.52 | 2.35 | |||||||
2009-07-20 | Tank 21 blank | Control | 8.28 | 29.96 | 22.76 | ||||||||||||||||||||||
2009-07-20 | Tank 22 blank | Low pH | 7.96 | 30.34 | 22.47 | ||||||||||||||||||||||
2009-09-23 | Tank 1 Clam | Low pH | 7.84 | 30.04 | 22.90 | 3925.3 | 3745.8 | 48.7 | 1717.6 | 4.6 | 3.0 | 0.2680 | 0.0306 | 0.1177 | 28 | 16.03 | 14.65 | 55.94 | 8 | 38.30 | 1249.04 | 1245.00 | 3450.61 | 211.92 | 3700.8 | 3.42 | 5.28 |
2009-09-23 | Tank 2 Clam | Very low pH | 7.57 | 30.43 | 22.57 | 3906.5 | 3868.5 | 93.3 | 3372.9 | 2.6 | 1.7 | 0.3189 | 0.0382 | 0.1107 | 29 | 19.10 | 24.39 | 51.84 | 8 | 75.39 | 2441.98 | 2434.06 | 3637.87 | 119.70 | 3833.0 | 1.92 | 2.97 |
2009-09-23 | Tank 3 Clam | Control | 8.13 | 30.42 | 22.50 | 3910.5 | 3525.8 | 22.4 | 798.3 | 8.1 | 5.3 | 0.2537 | 0.0201 | 0.0931 | 44 | 9.95 | 8.42 | 64.09 | 8 | 17.64 | 570.32 | 568.47 | 3085.98 | 367.64 | 3471.2 | 5.91 | 9.12 |
2009-09-23 | Tank 4 Algae | Low pH | 7.83 | 30.05 | 22.86 | 3919.2 | 3742.7 | 49.4 | 1764.2 | 4.5 | 3.0 | 8 | 39.28 | 1279.43 | 1275.30 | 3455.16 | 207.12 | 3701.6 | 3.34 | 5.16 | |||||||
2009-09-23 | Tank 5 Clam | Very low pH | 7.57 | 30.02 | 22.87 | 3905.2 | 3872.2 | 94.9 | 3341.4 | 2.6 | 1.7 | 0.2367 | 0.0269 | 0.1214 | 37 | 20.85 | 24.33 | 71.99 | 8 | 75.24 | 2451.10 | 2443.18 | 3636.77 | 119.75 | 3831.8 | 1.93 | 2.98 |
2009-09-23 | Tank 6 Algae | Control | 8.21 | 30.06 | 22.83 | 3933.8 | 3491.3 | 18.5 | 641.9 | 9.2 | 6.0 | 8 | 14.11 | 459.34 | 457.86 | 2976.42 | 427.63 | 3418.2 | 6.89 | 10.65 | |||||||
2009-09-23 | Tank 7 Clam | Low pH | 7.79 | 30.03 | 22.87 | 3917.9 | 3764.9 | 54.7 | 1951.6 | 4.2 | 2.7 | 0.3202 | 0.0433 | 0.1287 | 25 | 30.77 | 33.89 | 71.52 | 8 | 43.51 | 1417.43 | 1412.85 | 3490.43 | 190.79 | 3724.7 | 3.08 | 4.75 |
2009-09-23 | Tank 8 Algae | Very low pH | 7.44 | 30.43 | 22.52 | 3927.9 | 3962.7 | 131.0 | 4600.7 | 1.9 | 1.3 | 8 | 104.23 | 3371.54 | 3360.59 | 3724.51 | 90.68 | 3919.4 | 1.46 | 2.25 | |||||||
2009-09-23 | Tank 9 Clam | Control | 8.17 | 30.10 | 22.85 | 3915.6 | 3501.1 | 20.3 | 715.6 | 8.7 | 5.7 | 0.2616 | 0.0256 | 0.1139 | 67 | 35.93 | 36.60 | 85.12 | 8 | 15.72 | 511.98 | 510.32 | 3025.84 | 397.18 | 3438.7 | 6.40 | 9.89 |
2009-09-23 | Tank 10 Clam | Low pH | 7.83 | 30.05 | 21.91 | 3915.6 | 3736.2 | 48.9 | 1768.4 | 4.6 | 3.0 | 0.3031 | 0.0345 | 0.1124 | 19 | 26.88 | 33.52 | 79.73 | 8 | 40.17 | 1275.80 | 1271.63 | 3465.95 | 200.56 | 3706.7 | 3.22 | 4.98 |
2009-09-23 | Tank 11 Algae | Very low pH | 7.51 | 29.70 | 23.10 | 3922.2 | 3919.4 | 110.2 | 3886.6 | 2.3 | 1.5 | 8 | 87.44 | 2861.06 | 2851.84 | 3685.51 | 105.73 | 3878.7 | 1.71 | 2.64 | |||||||
2009-09-23 | Tank 12 Clam | Control | 8.19 | 29.36 | 23.09 | 3904.6 | 3473.4 | 19.1 | 675.3 | 9.0 | 5.9 | 0.2712 | 0.0329 | 0.1346 | 38 | 20.35 | 19.20 | 77.23 | 8 | 14.90 | 486.45 | 484.88 | 2993.63 | 407.34 | 3415.9 | 6.59 | 10.20 |
2009-09-23 | Tank 13 Algae | Low pH | 7.85 | 30.13 | 22.80 | 3926.1 | 3742.1 | 47.6 | 1672.4 | 4.6 | 3.0 | 8 | 37.38 | 1216.26 | 1212.32 | 3442.11 | 216.02 | 3695.5 | 3.48 | 5.38 | |||||||
2009-09-23 | Tank 14 blank | Very low pH | 7.67 | 30.04 | 22.80 | 3942.1 | 3862.2 | 76.1 | 2626.0 | 3.1 | 2.0 | 8 | 59.36 | 1930.46 | 1924.21 | 3607.87 | 149.25 | 3816.5 | 2.41 | 3.72 | |||||||
2009-09-23 | Tank 15 Algae | Control | 8.22 | 30.06 | 22.85 | 3911.5 | 3462.4 | 17.9 | 620.6 | 9.3 | 6.1 | 8 | 13.62 | 443.73 | 442.30 | 2941.88 | 432.83 | 3388.3 | 6.98 | 10.78 | |||||||
2009-09-23 | Tank 17 blank | Very low pH | 7.53 | 30.01 | 22.85 | 3909.6 | 3903.5 | 106.6 | 3714.3 | 2.3 | 1.5 | 8 | 83.14 | 2706.98 | 2698.23 | 3663.28 | 109.90 | 3856.3 | 1.77 | 2.74 | |||||||
2009-09-23 | Tank 18 blank | Control | 8.25 | 30.01 | 23.13 | 3920.2 | 3433.6 | 16.2 | 575.2 | 10.1 | 6.6 | 8 | 12.43 | 407.71 | 406.40 | 2890.53 | 459.86 | 3362.8 | 7.42 | 11.46 | |||||||
2009-09-23 | Tank 19 blank | Low pH | 8.00 | 29.95 | 22.82 | 3935.9 | 3656.3 | 32.4 | 1150.2 | 6.3 | 4.1 | 8 | 25.30 | 822.96 | 820.30 | 3286.28 | 290.20 | 3601.8 | 4.68 | 7.23 | |||||||
2009-09-23 | Tank 20 Clam | Very low pH | 7.46 | 30.08 | 22.82 | 3922.5 | 3944.2 | 123.9 | 4392.6 | 2.0 | 1.3 | 0.2906 | 0.0302 | 0.1190 | 28 | 23.58 | 27.70 | 73.16 | 8 | 98.91 | 3219.22 | 3208.82 | 3710.06 | 94.80 | 3903.8 | 1.53 | 2.36 |
2009-09-23 | Tank 21 blank | Control | 8.28 | 29.96 | 22.76 | 3928.1 | 3409.8 | 14.9 | 527.1 | 10.7 | 7.0 | 8 | 11.55 | 375.04 | 373.82 | 2854.87 | 479.43 | 3345.8 | 7.73 | 11.94 | |||||||
2009-09-23 | Tank 22 blank | Low pH | 7.96 | 30.34 | 22.47 | 3932.5 | 3675.9 | 35.7 | 1263.9 | 5.8 | 3.8 | 8 | 28.22 | 911.23 | 908.27 | 3332.73 | 267.59 | 3628.5 | 4.30 | 6.64 |