Citation:

bg-7-2915-2010

Name: Seawater carbonate chemistry and diatom Phaeodactylum tricornutum (CCMA 106) biological processes during experiments, 2010
Published: 2010
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Ochrophyta; Pelagos; Phaeodactylum tricornutum; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species

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Abstract:

CO2/pH perturbation experiments were carried out under two different pCO2 levels (39.3 and 101.3 Pa) to evaluate effects of CO2-induced ocean acidification on the marine diatom Phaeodactylum tricornutum. After acclimation (>20 generations) to ambient and elevated CO2 conditions (with corresponding pH values of 8.15 and 7.80, respectively), growth and photosynthetic carbon fixation rates of high CO2 grown cells were enhanced by 5% and 12%, respectively, and dark respiration stimulated by 34% compared to cells grown at ambient CO2. The half saturation constant (Km) for carbon fixation (dissolved inorganic carbon, DIC) increased by 20% under the low pH and high CO2 condition, reflecting a decreased affinity for HCO3- or/and CO2 and down-regulated carbon concentrating mechanism (CCM). In the high CO2 grown cells, the electron transport rate from photosystem II (PSII) was photoinhibited to a greater extent at high levels of photosynthetically active radiation, while non-photochemical quenching was reduced compared to low CO2 grown cells. This was probably due to the down-regulation of CCM, which serves as a sink for excessive energy. The balance between these positive and negative effects on diatom productivity will be a key factor in determining the net effect of rising atmospheric CO2 on ocean primary production.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Ochrophyta; Pelagos; Phaeodactylum tricornutum; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species

Project(s):

European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)

European Project on Ocean Acidification (EPOCA)

Ocean Acidification International Coordination Centre (OA-ICC)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Sixth Framework Programme (FP6), grant/award no. 511106 : European network of excellence for Ocean Ecosystems Analysis

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Experimental treatment	Exp treat		Wu, YaPing
2	Comment	Comment		Wu, YaPing
3	Salinity	Sal		Wu, YaPing	Measured
4	Temperature, water	Temp	°C	Wu, YaPing	Measured
5	Carbon dioxide, partial pressure	pCO2	Pa	Wu, YaPing	Infrared CO2/H2O gas analyzer, non-dispersive, LI-COR type LI-7000
6	Carbon dioxide, partial pressure, standard deviation	pCO2 std dev	±	Wu, YaPing
7	pH	pH		Wu, YaPing	pH meter (Benchtop pH510, OAKTON)	NBS scale
8	pH, standard deviation	pH std dev	±	Wu, YaPing
9	Carbon, inorganic, dissolved	DIC	µmol/kg	Wu, YaPing	DIC analyzer (AS-C3, Apollo Scitech)
10	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Wu, YaPing
11	Bicarbonate ion	[HCO3]-	µmol/kg	Wu, YaPing	Calculated using CO2SYS
12	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Wu, YaPing
13	Carbonate ion	[CO3]2-	µmol/kg	Wu, YaPing	Calculated using CO2SYS
14	Carbonate ion, standard deviation	[CO3]2- std dev	±	Wu, YaPing
15	Carbon dioxide	CO2	µmol/kg	Wu, YaPing	Calculated using CO2SYS
16	Carbon dioxide, standard deviation	CO2 std dev	±	Wu, YaPing
17	Alkalinity, total	AT	µmol/kg	Wu, YaPing	Calculated using CO2SYS
18	Alkalinity, total, standard deviation	AT std dev	±	Wu, YaPing
19	Photochemical efficiency	delta F/Fm	arbitrary units	Wu, YaPing	Calculated, see reference(s)
20	Photochemical efficiency, standard deviation	delta F/Fm std dev	±	Wu, YaPing
21	Maximum potential photosynthesis	Pmax		Wu, YaPing	see reference(s)
22	Maximum potential photosynthesis, standard deviation	Pmax std dev	±	Wu, YaPing
23	Light saturation point	Ik		Wu, YaPing	see reference(s)
24	Light saturation point, standard deviation	Ik std dev	±	Wu, YaPing
25	Chlorophyll a per cell	Chl a/cell	pg/#	Wu, YaPing	Spectrophotometry
26	Chlorophyll a, standard deviation	Chl a std dev	±	Wu, YaPing
27	Growth rate	µ	#/day	Wu, YaPing	Calculated
28	Growth rate, standard deviation	µ std dev	±	Wu, YaPing
29	Photosynthetic carbon fixation rate	C fix	µmol/µg/h	Wu, YaPing	Liquid scintillation counter (Tri-Carb 2800TR, Perkin-Elmer)
30	Photosynthetic carbon fixation rate, standard deviation	C fix std dev	±	Wu, YaPing
31	Respiration rate, oxygen	Resp O2	µmol/mg/h	Wu, YaPing	Clark type oxygen electrode (5300A, YSI)	Dark
32	Respiration rate, oxygen, standard deviation	Resp O2 std dev	±	Wu, YaPing	Calculated
33	Carbonate system computation flag	CSC flag		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
34	pH	pH		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Total scale
35	Carbon dioxide	CO2	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
36	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
37	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
38	Bicarbonate ion	[HCO3]-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
39	Carbonate ion	[CO3]2-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
40	Aragonite saturation state	Omega Arg		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
41	Calcite saturation state	Omega Cal		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

152 data points

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