Citation:

epic.19376.d001

Name: Table 1: Densities of nitrate-dissimilating and nitrite-dissimilating bacteria in surface sediments in the German Bight, North Sea
Published: 1979
License: https://creativecommons.org/licenses/by/3.0/

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Abstract:

At 24 stations in the Weser Estuary and the German Bight the Most Probable Numbers (MPN/g dry wt. sediment) of nitrate-dissimilating (= denitrifying) and of nitrate plus nitrite-dissimilating bacteria were recorded. The numbers of nitrite-dissimilating bacteria, i. e. denitrifiers not capable of reducing nitrate to nitrite, were calculated by subtraction of the MPN for nitrate-dissimilating from the MPN of nitrate plus nitrite-dissimilating bacteria. By determining the percentages of these bacteria in relation to the number of the heterotrophs, the ecological importance of denitrification, especially the nitrite dissimilation, was estimated. The results showed the MPN of nitrate-dissimilating bacteria to be in the range of 0-156 (up to 0.8 % of heterotrophic bacteria). An exception was the sediment of one station with a MPN of 1849, or 5.2 % of the heterotrophs. The amounts of nitrite-dissimilating bacteria were between 0 and 2352 (up to 13 % of heterotrophic bacteria). In the estuary the numbers of nitrate-dissimilating and of nitrite-dissimilating bacteria showed a decreasing tendency with distance from Bremerhaven. The highest numbers were found in the Weser off Bremerhaven and also at 3 stations in the German Bight, south of the Isle of Helgoland.

Coverage:

Median Latitude: 53.742046 * Median Longitude: 8.289608 * South-bound Latitude: 53.527000 * West-bound Longitude: 7.905000 * North-bound Latitude: 54.161800 * East-bound Longitude: 8.557900

Date/Time Start: 1977-09-14T12:00:00 * Date/Time End: 1977-11-22T12:00:00

Minimum DEPTH, sediment/rock: 0 m * Maximum DEPTH, sediment/rock: 0 m

Event(s):

VH_09_77_1 (Buoy 54) * Latitude: 53.527000 * Longitude: 8.557900 * Date/Time: 1977-09-14T12:00:00 * Elevation: -8.0 m * Location: North Sea * Campaign: * Basis: * Method/Device: van Veen Grab (VGRAB)

VH_09_77_2 (Buoy 54) * Latitude: 53.553900 * Longitude: 8.557900 * Date/Time: 1977-09-14T12:00:00 * Elevation: -15.0 m * Location: North Sea * Campaign: * Basis: * Method/Device: van Veen Grab (VGRAB)

VH_09_77_3 * Latitude: 53.583300 * Longitude: 8.516700 * Date/Time: 1977-09-14T12:00:00 * Elevation: -14.0 m * Location: North Sea * Campaign: * Basis: * Method/Device: van Veen Grab (VGRAB)

Comment:

The basal medium consisted of Bacto Nutrient Broth (DIFCO) with the addition of 17.6 g/l NaCl. This medium, abbreviated as NB in the parameter comment, was used to determine the Most Probable Numbers (MPN) of heterotrophic bacteria, whereas the addition of 0.5 % KN03 (NB +KN03 ) or 0.2 % KN02 (NB + KN02 ) made possible the records of nitrate-dissimilating and of nitrate plus nitrite-dissimilating bacteria, respectively. The MPN obtained with a fourth medium containing NB, 0.5 % KN03 and 0.2 % KN02 (NB + KN03 + KN02 ) were compared with the MPN from NB + KN02. The media were filled in 9 ml portions in test tubes and in each test tube a Durham tube was inserted for the detection of gas formation. The media were sterilized at 121 °C for 20 minutes in an autoclave.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event
2	Sample code/label	Sample label		Tan, Tjhing Lok
3	Optional event label	Event 2
4	Date/Time of event	Date/Time
5	Latitude of event	Latitude
6	Longitude of event	Longitude
7	Elevation of event	Elevation	m
8	DEPTH, sediment/rock	Depth sed	m			Geocode
9	Sediment type	Sediment		Tan, Tjhing Lok
10	Bacteria, heterotrophic	Bact het	#/g	Tan, Tjhing Lok	Most probable number method (MPN), based on serial dilutions	NB: turbidity
11	Bacteria, nitrate-reducing	Bact NO3-red	#/g	Tan, Tjhing Lok	Most probable number method (MPN), based on serial dilutions	NB+KNO3: nitrite
12	Bacteria, nitrate-dissimilating	Bact NO3-dis	#/g	Tan, Tjhing Lok	Most probable number method (MPN), based on serial dilutions	NB+KNO3: gas
13	Bacteria, nitrate + nitrite dissimilating	Bact NO3- + NO2-dis	#/g	Tan, Tjhing Lok	Most probable number method (MPN), based on serial dilutions	NB+KNO2: gas
14	Bacteria, nitrate + nitrite dissimilating	Bact NO3- + NO2-dis	#/g	Tan, Tjhing Lok	Most probable number method (MPN), based on serial dilutions	NB+KNO3+KNO2: gas
15	Bacteria, nitrite-dissimilating	Bact NO2-dis	#/g	Tan, Tjhing Lok	Most probable number method (MPN), based on serial dilutions

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Size:

192 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Event	2 Sample label	3 Event 2	4 Date/Time	5 Latitude	6 Longitude	7 Elevation [m]	9 Sediment	10 Bact het [#/g] (NB: turbidity)	11 Bact NO3-red [#/g] (NB+KNO3: nitrite)	12 Bact NO3-dis [#/g] (NB+KNO3: gas)	13 Bact NO3- + NO2-dis [#/g] (NB+KNO2: gas)	14 Bact NO3- + NO2-dis [#/g] (NB+KNO3+KNO2: gas)	15 Bact NO2-dis [#/g]
VH_09_77_1	1	Buoy 54	1977-09-14	53.5270	8.5579	-8	silt	>20397	>20397	78	445	445	367
VH_11_77_1	1*	Buoy 54	1977-11-22	53.5270	8.5579	-9	gravel	35564	21812	1849	356	356	-1493
VH_09_77_2	2	Buoy 54	1977-09-14	53.5539	8.5579	-15	fine sand	>6620	>6620	5	144	277	272
VH_09_77_3	3		1977-09-14	53.5833	8.5167	-14	silt	>18395	>18395	156	2508	38	2352
VH_11_77_3	3*		1977-11-22	53.5833	8.5167	-17	coarse and fine sand	493	1272	0	48	19	48
VH_09_77_4	4	Buoy 49	1977-09-14	53.6029	8.5000	-13	fine sand	7646	7646	0	10	3	10
VH_09_77_5	5	Buoy 48	1977-09-14	53.6225	8.4752	-11	fine sand	5976	5976	12	51	2	39
VH_11_77_5	5*	Buoy 48	1977-11-22	53.6225	8.4752	-13	fine sand	8080	2655	54	139	12	85
VH_09_77_6	6	Buoy 44	1977-09-14	53.6422	8.4339	-14	fine sand	6986	2921	15	27	2	12
VH_09_77_7	7	Buoy 38	1977-09-14	53.6642	8.3884	-13	fine sand	7279	7279	0	0	20	20
VH_09_77_8	8	Buoy 34	1977-09-14	53.6814	8.3595	-13	sand	2408	>5759	2	8	5	6
VH_09_77_9	9	Buoy 30	1977-09-14	53.6936	8.3347	-18	sand	2579	2579	2	13	0	11
VH_11_77_9	9*	Buoy 30	1977-11-22	53.6936	8.3347	-18	fine sand	1376	1376	0	21	0	21
VH_09_77_10	10	Buoy 27	1977-09-14	53.7034	8.3017	-15	sand	894	6557	0	0	2	2
VH_09_77_11	11	Buoy 23	1977-09-14	53.7255	8.2479	-15	sand	621	3070	0	0	0	0
VH_09_77_12	12	Buoy 19	1977-09-14	53.7475	8.1942	-7	silt	>12108	>12108	4	4	0	0
VH_09_77_13	13	Buoy 15	1977-09-14	53.7794	8.1529	-23	fine sand	>4065	4065	0	1	0	1
VH_09_77_14	14	Buoy 9	1977-09-14	53.8186	8.0868	-19	coarse sand	1571	>7201	0	0	0	0
VH_11_77_15	15	Buoy A/7	1977-11-22	53.8725	8.0868	-19	fine sand	4512	2232	0	2	0	2
VH_11_77_16	16	Buoy A/2	1977-11-22	53.9069	7.9959	-13	fine sand	1208	1260	0	0	0	0
VH_11_77_17	17	Nordergründe	1977-11-22	53.9461	8.0041	-24	sandy silt	138114	264718	2	2	0	0
VH_11_77_18	18		1977-11-22	54.0500	7.9833	-29	silt	48087	48087	10	78	10	68
VH_11_77_19	19		1977-11-22	54.1000	7.9833	-30	silt	602094	31414	5	30	122	117
VH_11_77_20	20	Buoy 3	1977-11-22	54.1618	7.9050	-10	fine sand	5732	14792	6	148	573	567