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Kranz, Sven A; Sültemeyer, Dieter; Richter, Klaus-Uwe; Rost, Björn (2009): Seawater carbonate chemistry and processes during experiments with cyanobacterium Trichodesmium (IMS101), 2009. PANGAEA, https://doi.org/10.1594/PANGAEA.736024, Supplement to: Kranz, SA et al. (2009): Carbon acquisition by Trichodesmium: the effect of pCO2 and diurnal changes. Limnology and Oceanography, 54(2), 548-559, https://doi.org/10.4319/lo.2009.54.2.0548

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Abstract:
We investigated carbon acquisition by the N2-fixing cyanobacterium Trichodesmium IMS101 in response to CO2 levels of 15.1, 37.5, and 101.3 Pa (equivalent to 150, 370, and 1000 ppm). In these acclimations, growth rates as well as cellular C and N contents were measured. In vivo activities of carbonic anhydrase (CA), photosynthetic O2 evolution, and CO2 and HCO3- fluxes were measured using membrane inlet mass spectrometry and the 14C disequilibrium technique. While no differences in growth rates were observed, elevated CO2 levels caused higher C and N quotas and stimulated photosynthesis and N2 fixation. Minimal extracellular CA (eCA) activity was observed, indicating a minor role in carbon acquisition. Rates of CO2 uptake were small relative to total inorganic carbon (Ci) fixation, whereas HCO{3 contributed more than 90% and varied only slightly over the light period and between CO2 treatments. The low eCA activity and preference for HCO3- were verified by the 14C disequilibrium technique. Regarding apparent affinities, half-saturation concentrations (K1/2) for photosynthetic O2 evolution and HCO3- uptake changed markedly over the day and with CO2 concentration. Leakage (CO2 efflux : Ci uptake) showed pronounced diurnal changes. Our findings do not support a direct CO2 effect on the carboxylation efficiency of ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) but point to a shift in resource allocation among photosynthesis, carbon acquisition, and N2 fixation under elevated CO2 levels. The observed increase in photosynthesis and N2fixation could have potential biogeochemical implications, as it may stimulate productivity in N-limited oligotrophic regions and thus provide a negative feedback in rising atmospheric CO2 levels.
Keyword(s):
Bacteria; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Cyanobacteria; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Single species; Trichodesmium sp.
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Experimental treatmentExp treatKranz, Sven A
2SalinitySalKranz, Sven AConductivity meter (WTW, Weilheim, Gemany)
3Temperature, waterTemp°CKranz, Sven A
4Radiation, photosynthetically activePARµmol/m2/sKranz, Sven AWalz 4pi sensor
5Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
6Phosphate[PO4]3-µmol/lKranz, Sven A
7Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmKranz, Sven A
8Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmKranz, Sven A
9pHpHKranz, Sven ApH, ElectrodeNBS scale
10pH, standard deviationpH std dev±Kranz, Sven ANBS scale
11pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
12Carbon, inorganic, dissolvedDICµmol/lKranz, Sven ACalculated using CO2SYS
13Carbon, inorganic, dissolved, standard deviationDIC std dev±Kranz, Sven A
14Carbon, inorganic, dissolvedDICµmol/kgKranz, Sven AOriginal unit in µmol/l, calculated using density
15Alkalinity, totalATmmol(eq)/lKranz, Sven AAlkalinity, Gran titration (Gran, 1950)
16Alkalinity, total, standard deviationAT std dev±Kranz, Sven A
17Alkalinity, totalATµmol/kgKranz, Sven ACalculatedOriginal unit in µmol/l, calculated using density
18Carbon dioxide, totalTCO2µmol/lKranz, Sven ACalculated using CO2SYS
19Carbon dioxide, standard deviationCO2 std dev±Kranz, Sven A
20Carbon dioxideCO2µmol/kgKranz, Sven ACalculated
21Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
22Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
23Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
24Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
25Growth rateµ#/dayKranz, Sven ABased on changes in chla/cells/POC/PON
26Growth rate, standard deviationµ std dev±Kranz, Sven A
27Particulate organic nitrogen per cellPON/cellpmol/#Kranz, Sven AMass spectrometer ANCA-SL 20-20 Europa Scientific
28Particulate organic nitrogen per cell, standard deviationPON/cell std dev±Kranz, Sven A
29Particulate organic carbon, per cellPOC/cellpmol/#Kranz, Sven AMass spectrometer ANCA-SL 20-20 Europa Scientific
30Particulate organic carbon content per cell, standard deviationPOC cont/cell std dev±Kranz, Sven A
31Carbon/Nitrogen ratioC/NKranz, Sven A
32Carbon/Nitrogen ratio, standard deviationC/N std dev±Kranz, Sven A
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
96 data points

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