Data Description

  RIS BibTeX
Citation:
Trimborn, S et al. (2010): Seawater carbonate chemistry and biological processes during experiments with four species of marine diatoms. doi:10.1594/PANGAEA.733948,
Supplement to: Trimborn, Scarlett; Wolf-Gladrow, Dieter; Richter, Klaus-Uwe; Rost, Björn (2009): The effect of pCO2 on carbon acquisition and intracellular assimilation in four marine diatoms. Journal of Experimental Marine Biology and Ecology, 376(1), 26-36, doi:10.1016/j.jembe.2009.05.017
Abstract:
The effect of pCO2 on carbon acquisition and intracellular assimilation was investigated in the three bloom-forming diatom species, Eucampia zodiacus (Ehrenberg), Skeletonema costatum (Greville) Cleve, Thalassionema nitzschioides (Grunow) Mereschkowsky and the non-bloom-forming Thalassiosira pseudonana (Hust.) Hasle and Heimdal. In vivo activities of carbonic anhydrase (CA), photosynthetic O2 evolution, CO2 and HCO3? uptake rates were measured by membrane-inlet mass spectrometry (MIMS) in cells acclimated to pCO2 levels of 370 and 800 ?atm. To investigate whether the cells operate a C4-like pathway, activities of ribulose-1,5-bisphosphate carboxylase (RubisCO) and phosphoenolpyruvate carboxylase (PEPC) were measured at the mentioned pCO2 levels and a lower pCO2 level of 50 ?atm. In the bloom-forming species, extracellular CA activities strongly increased with decreasing CO2 supply while constantly low activities were obtained for T. pseudonana. Half-saturation concentrations (K1/2) for photosynthetic O2 evolution decreased with decreasing CO2 supply in the two bloom-forming species S. costatum and T. nitzschioides, but not in T. pseudonana and E. zodiacus. With the exception of S. costatum, maximum rates (Vmax) of photosynthesis remained constant in all investigated diatom species. Independent of the pCO2 level, PEPC activities were significantly lower than those for RubisCO, averaging generally less than 3%. All examined diatom species operate highly efficient CCMs under ambient and high pCO2, but differ strongly in the degree of regulation of individual components of the CCM such as Ci uptake kinetics and extracellular CA activities. The present data do not suggest C4 metabolism in the investigated species.
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Experimental treatment *Exp trtmTrimborn, Scarlett *
2Species *SpeciesTrimborn, Scarlett *
3Salinity *SalTrimborn, Scarlett *
4Temperature, water *Temp°CTrimborn, Scarlett *
5Light:Dark cycle *L:Dhh:hhTrimborn, Scarlett *
6Radiation, photosynthetically active *PARµE/m2/sTrimborn, Scarlett *
7pH *pHTrimborn, Scarlett *pH meter (WTW pH 3000) *NBS scale
8pH *pHNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *Total scale
9Alkalinity, total *ATµmol/kgTrimborn, Scarlett *Alkalinity, Gran titration (Gran, 1950) *
10Total carbon *TCµmol/kgTrimborn, Scarlett *Calculated using CO2SYS *
11Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmTrimborn, Scarlett *Calculated using CO2SYS *
12Carbon dioxide *CO2µmol/kgTrimborn, Scarlett *Calculated using CO2SYS *
13Carbonate system computation flag *CSC flagNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
14Carbon dioxide *CO2µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
15Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
16Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
17Bicarbonate ion *[HCO3]-µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
18Carbonate ion *[CO3]2-µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
19Aragonite saturation state *Omega ArgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
20Calcite saturation state *Omega CalNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
21Extracellular carbonic anhydrase activity *eCA activityU/µg Chl aTrimborn, Scarlett *Measured by loss of 18O (Silverman, 1982) *
22Intracellular carbonic anhydrase activity *iCA activitydelta/µg Chl aTrimborn, Scarlett *Measured by loss of 18O (Silverman, 1982) *
23Bicarbonate uptake *[HCO3]- upt%Trimborn, Scarlett *Calculated *
24Isotope fraction *epsilon pper milTrimborn, Scarlett *Calculated after Freeman & Hayes (1992) *
25Bicarbonate uptake *[HCO3]- upt%Trimborn, Scarlett *see reference(s) *
License:
Size:
1263 data points

Download Data

Download dataset as tab-delimited text (use the following character encoding: )

View dataset as HTML