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Langer, Gerald; Nehrke, Gernot; Probert, Ian; Ly, J; Ziveri, Patrizia (2009): Seawater carbonate chemistry and biological processes during experiments with four strains of Emiliania huxleyi [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.733946, Supplement to: Langer, G et al. (2009): Strain-specific responses of Emiliania huxleyi to changing seawater carbonate chemistry. Biogeosciences, 6(11), 2637-2646, https://doi.org/10.5194/bg-6-2637-2009

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Abstract:
Four strains of the coccolithophore E. huxleyi (RCC1212, RCC1216, RCC1238, RCC1256) were grown in dilute batch culture at four CO2 levels ranging from ~200 µatm to ~1200 µatm. Growth rate, particulate organic carbon content, and particulate inorganic carbon content were measured, and organic and inorganic carbon production calculated. The four strains did not show a uniform response to carbonate chemistry changes in any of the analysed parameters and none of the four strains displayed a response pattern previously described for this species. We conclude that the sensitivity of different strains of E. huxleyi to acidification differs substantially and that this likely has a genetic basis. We propose that this can explain apparently contradictory results reported in the literature.
Keyword(s):
Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species
Further details:
Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Coverage:
Median Latitude: 5.162500 * Median Longitude: 76.658333 * South-bound Latitude: -42.300000 * West-bound Longitude: -20.333330 * North-bound Latitude: 63.400000 * East-bound Longitude: 169.833330
Date/Time Start: 1998-09-01T00:00:00 * Date/Time End: 2005-11-30T00:00:00
Event(s):
North_Atlantic_OA * Latitude: 63.400000 * Longitude: -20.333330 * Date/Time Start: 1999-07-01T00:00:00 * Date/Time End: 1999-07-31T00:00:00 * Method/Device: Experiment (EXP) * Comment: Isolation date and location of culture strains
North_Pacific_OA * Latitude: 34.016670 * Longitude: 139.833330 * Date/Time Start: 2005-11-01T00:00:00 * Date/Time End: 2005-11-30T00:00:00 * Method/Device: Experiment (EXP) * Comment: Isolation date and location of culture strains
South_Atlantic_OA * Latitude: -34.466670 * Longitude: 17.300000 * Date/Time Start: 2000-09-01T00:00:00 * Date/Time End: 2000-09-30T00:00:00 * Method/Device: Experiment (EXP) * Comment: Isolation date and location of culture strains
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEvent
2IdentificationIDLanger, Gerald
3Carbonate system computation flagCSC flagLanger, Gerald
4SalinitySalLanger, GeraldConductivity meter (WTW, Weilheim, Gemany)
5Temperature, waterTemp°CLanger, Gerald
6Radiation, photosynthetically activePARµmol/m2/sLanger, Gerald
7Nitrate[NO3]-µmol/lLanger, Gerald
8Phosphate[PO4]3-µmol/lLanger, Gerald
9Alkalinity, totalATµmol/kgLanger, GeraldAlkalinity, Gran titration (Gran, 1950)
10Carbon, inorganic, dissolvedDICµmol/kgLanger, GeraldPhotometry
11Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmLanger, GeraldCalculated
12pHpHLanger, GeraldCalculated using CO2SYSNBS scale
13Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
14pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)total scale
15Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
16Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
17Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
18Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
19Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
20Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
21Growth rateµ#/dayLanger, Gerald
22Growth rate, standard deviationµ std dev±Langer, Gerald
23Particulate inorganic carbon per cellPIC/cellpmol/#Langer, GeraldElement analyser CNS, EURO EA
24Particulate inorganic carbon per cell, standard deviationPIC/cell std dev±Langer, GeraldElement analyser CNS, EURO EA
25Particulate organic carbon, per cellPOC/cellpmol/#Langer, GeraldElement analyser CNS, EURO EA
26Particulate organic carbon content per cell, standard deviationPOC cont/cell std dev±Langer, GeraldElement analyser CNS, EURO EA
27Particulate inorganic carbon production per cellPIC prod/cellpmol/#/dayLanger, GeraldElement analyser CNS, EURO EA
28Particulate inorganic carbon, production, standard deviationPIC prod std dev±Langer, GeraldElement analyser CNS, EURO EA
29Particulate organic carbon production per cellPOC prod/cellpmol/#/dayLanger, GeraldElement analyser CNS, EURO EA
30Particulate organic carbon, production, standard deviationPOC prod std dev±Langer, GeraldElement analyser CNS, EURO EA
31Particulate inorganic carbon/particulate organic carbon ratioPIC/POCLanger, Gerald
32Particulate inorganic carbon/particulate organic carbon ratio, standard deviationPIC/POC ratio std dev±Langer, Gerald
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
496 data points

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