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Hansen, Per Juel; Lundholm, Nina; Rost, Björn (2007): Seawater carbonate chemistry and growth rate during experiments with dinoflagellates, 2007. PANGAEA, https://doi.org/10.1594/PANGAEA.718182, In supplement to: Hansen, PJ et al. (2007): Growth limitation in marine red-tide dinoflagellates: effects of pH versus inorganic carbon availability. Marine Ecology Progress Series, 334, 63-71, https://doi.org/10.3354/meps334063

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Abstract:
The effects of dissolved inorganic carbon (DIC) on the growth of 3 red-tide dinoflagellates (Ceratium lineatum, Heterocapsa triquetra and Prorocentrum minimum) were studied at pH 8.0 and at higher pH levels, depending upon the pH tolerance of the individual species. The higher pH levels chosen for experiments were 8.55 for C. lineatum and 9.2 for the other 2 species. At pH 8.0, which approximates the pH found in the open sea, the maximum growth in all species was maintained until the total DIC concentration was reduced below ~0.4 and 0.2 mM for C. lineatum and the other 2 species, respectively. Growth compensation points (concentration of inorganic carbon needed for maintenance of cells) were reached at ~0.18 and 0.05 mM DIC for C. lineatum and the other 2 species, respectively. At higher pH levels, maximum growth rates were lower compared to growth at pH 8, even at very high DIC concentrations, indicating a direct pH effect on growth. Moreover, the concentration of bio-available inorganic carbon (CO2 + HCO3-) required for maintenance as well as the half-saturation constants were increased considerably at high pH compared to pH 8.0. Experiments with pH-drift were carried out at initial concentrations of 2.4 and 1.2 mM DIC to test whether pH or DIC was the main limiting factor at a natural range of DIC. Independent of the initial DIC concentrations, growth rates were similar in both incubations until pH had increased considerably. The results of this study demonstrated that growth of the 3 species was mainly limited by pH, while inorganic carbon limitation played a minor role only at very high pH levels and low initial DIC concentrations.
Keyword(s):
Bottles or small containers/Aquaria (<20 L); Caratium lineatum; Chromista; Growth/Morphology; Heterocapsa triquetra; Laboratory experiment; Laboratory strains; Myzozoa; Pelagos; Phytoplankton; Prorocentrum minimum; Single species
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Event(s):
Hansen_etal_07/F1 * Method/Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesHansen, Per Juel
2Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
3Temperature, waterTemp°CHansen, Per Juel
4SalinitySalHansen, Per Juel
5Density, mass densityDensitykg/m3Hansen, Per JuelCalculated
6Radiation, photosynthetically activePARµmol/m2/sHansen, Per JuelPAR sensor LI-1000, LI-COR Inc.
7Light:Dark cycleL:Dhh:hhHansen, Per JuelMeasured
8pHpHHansen, Per JuelpH meter (Sentron, Argus X)NBS scale
9pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
10Carbon, inorganic, dissolvedDICµmol/kgHansen, Per JuelInfrared gas analyser (ADC, MK3)
11Alkalinity, totalATµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
12Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
13Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
14Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
15Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
16Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
17Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
18Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
19Growth rateµ#/dayHansen, Per JuelCalculated
Size:
1349 data points

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