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Gazeau, Frédéric; Taillandier, V; Alliouane, Samir; Gobert, Sylvie; Giani, Michele; Sallon, Amèlie; Celussi, Mauro; Rees, Andrew; Pitta, Paraskevi; Maugendre, Laure; Guieu, Cecile; Louis, Justine (2015): Stareso MedSeA mesocosm experiment 2012: perturbation studies [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.810331, In: Gazeau, F et al. (2012): Stareso MedSeA mesocosm experiment: field and perturbation studies 2012 [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.811018

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Keyword(s):
Coast and continental shelf; Community composition and diversity; Entire community; Field experiment; Mediterranean Sea; Mesocosm or benthocosm; Other metabolic rates; Pelagos; Primary production/Photosynthesis; Respiration; Temperate
Related to:
Maugendre, Laure; Gattuso, Jean-Pierre; Poulton, Alex J; Dellisanti, Walter; Gaubert, M; Guieu, Cecile; Gazeau, Frédéric (2015): No detectable effect of ocean acidification on plankton metabolism in the NW oligotrophic Mediterranean Sea: results from two mesocosm studies. Estuarine, Coastal and Shelf Science, https://doi.org/10.1016/j.ecss.2015.03.009
Further details:
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb
Funding:
Seventh Framework Programme (FP7), grant/award no. 265103: Mediterranean Sea Acidification in a Changing Climate
Coverage:
Latitude: 42.580000 * Longitude: 8.726000
Date/Time Start: 2012-06-21T07:30:00 * Date/Time End: 2012-07-14T12:32:00
Minimum DEPTH, water, experiment: 0.75 m * Maximum DEPTH, water, experiment: 12.00 m
Event(s):
Stareso-2012-CTD-perturbation * Latitude: 42.580000 * Longitude: 8.726000 * Date/Time Start: 2012-06-20T00:00:00 * Date/Time End: 2012-07-14T00:00:00 * Location: Bay of Calvi, Mediterranean Sea * Method/Device: Mesocosm experiment (MESO)
Stareso-2012-IWS-perturbation * Latitude: 42.580000 * Longitude: 8.726000 * Date/Time Start: 2012-06-20T00:00:00 * Date/Time End: 2012-07-14T00:00:00 * Location: Bay of Calvi, Mediterranean Sea * Method/Device: Mesocosm experiment (MESO)
Comment:
<0= below detection limits. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-07-30.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEvent
2Other eventOther eventGazeau, Frédéric
3TreatmentTreatGazeau, Frédéric
4Day of experimentDOEdayGuieu, Cecile
5DATE/TIMEDate/TimeGeocode
6DEPTH, water, experimentDepth water expmGeocode
7Temperature, waterTemp°CTaillandier, VCTD
8SalinitySalTaillandier, VCTD
9Density, sigma, in situSigma in situkg/m3Taillandier, VCTD
10FluorometerFluorometerVTaillandier, VCTD
11OxygenO2µmol/lTaillandier, VCTD with attached oxygen sensor
12IrradianceEµmol/m2/sTaillandier, VCTD
13pHpHTaillandier, VCTDTotal scale; based on a empiric relationship between pH calculated from TA and TCO2 and integrated pH from the CTD (originally on the NBS
14Carbonate system computation flagCSC flagAlliouane, SamirCalculated using seacarb
15pHpHAlliouane, SamirCalculated using seacarbTotal scale
16Carbon dioxideCO2µmol/kgAlliouane, SamirCalculated using seacarb
17Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmAlliouane, SamirCalculated using seacarb
18Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmAlliouane, SamirCalculated using seacarb
19Bicarbonate ion[HCO3]-µmol/kgAlliouane, SamirCalculated using seacarb
20Carbonate ion[CO3]2-µmol/kgAlliouane, SamirCalculated using seacarb
21Carbon, inorganic, dissolvedDICµmol/kgAlliouane, SamirAIRICA analyzer (Miranda)
22Alkalinity, totalATµmol/kgAlliouane, SamirTitration potentiometric, Metrohm
23Aragonite saturation stateOmega ArgAlliouane, SamirCalculated using seacarb
24Calcite saturation stateOmega CalAlliouane, SamirCalculated using seacarb
25Nitrate[NO3]-µmol/lGobert, SylvieSkalar AutoAnalyser
26Ammonium[NH4]+µmol/lGobert, SylvieSkalar AutoAnalyser
27Phosphate[PO4]3-µmol/lGobert, SylvieSkalar AutoAnalyser
28SiliconSiµmol/lGobert, SylvieSkalar AutoAnalyser
29Nitrogen, total, particulateTPNµmol/lGiani, MicheleElement analyser CHN, Costech
30Carbon, total, particulateTPCµmol/lGiani, MicheleElement analyser CHN, Costech
31Nitrogen, organic, particulatePONµmol/lGiani, MicheleElement analyser CHN, Costech
32Carbon, organic, particulatePOCµmol/lGiani, MicheleElement analyser CHN, Costech
33Phosphorus, particulatePPµmol/lGiani, MicheleColorimetric, GF/F filters, autoanalyzer (Bran Luebbe)Total
34Chlorophyll c3Chl c3µg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
35Chlorophyll c1+c2Chl c1+c2µg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
36Chlorophyllide aChlide aµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)Sum
37PeridininPeridµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
38Phaeophorbide aPhaeopho aµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
3919-ButanoyloxyfucoxanthinBut-fucoµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
40FucoxanthinFucoµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
41NeoxanthinNeoµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
42PrasinoxanthinPrasµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
43ViolaxanthinViolaµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
4419-HexanoyloxyfucoxanthinHex-fucoµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
45DiadinoxanthinDiadinoµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
46AlloxanthinAlloµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
47DiatoxanthinDiatoµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
48ZeaxanthinZeaµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
49LuteinLutµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
50Bacteriochlorophyll aBChl aµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
51Divinyl chlorophyll bDV chl bµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
52Chlorophyll bChl bµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)Total
53Chlorophyll bChl bµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
54Divinyl chlorophyll aDV chl aµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
55Chlorophyll aChl aµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
56Chlorophyll aChl aµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)Total
57Phaeophytin aPhaeophytin aµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)Sum
58CaroteneCaroteneµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)Sum
59beta-glucosidase activityMUF-bgluµmol/l/hCelussi, Maurosee commentMUF-beta-D-glucose fluorogenic substrate
60Lipase activityLipase activitynmol/l/hCelussi, Maurosee commentMUF-oleate fluorogenic substrate
61Chitinase activityChitinase activitynmol/l/hCelussi, Maurosee commentMUF-N-acetyl-beta-D-glucosaminide fluorogenic substrate
62Phosphatase activityMUF-Phosµmol/l/hCelussi, Maurosee commentMUF-phosphate fluorogenic substrate
63Leucine aminopeptidase activityLeu aminopepnmol/l/hCelussi, Maurosee commentMCA-leucine fluorogenic substrate
64ProkaryotesProkaryotes#/mlCelussi, MauroEpifluorescence microscopyHighly active
65ProkaryotesProkaryotes#/mlCelussi, MauroEpifluorescence microscopyTotal
66Net community production of oxygenNCP O2µmol/l/dayGazeau, FrédéricIncubation (INCUB)Community; Incubated in the laboratory in the dark at in situ temperature in 5 x biological oxygen demand (BOD) 60ml bottles over 24h. Rate estimated using a linear regression between initial and final oxygen concentration
67Net community production of oxygen, standard errorNCP O2 std e±Gazeau, FrédéricCalculated from linear regression
68Respiration rate, oxygenResp O2µmol/l/dayGazeau, FrédéricIncubation (INCUB)Community; Incubated in the laboratory in the dark at in situ temperature in 5 x biological oxygen demand (BOD) 60ml bottles over 24h. Rate estimated using a linear regression between initial and final oxygen concentration
69Respiration rate, oxygen, standard deviationResp O2 std dev±Gazeau, FrédéricCalculated from linear regressionCommunity
70Gross community production of oxygenGCP O2µmol/l/dayGazeau, FrédéricCalculatedCalculated as the difference between net community production and respiration
71Gross community production of oxygen, standard errorGCP O2 std e±Gazeau, FrédéricCalculated using error propagation
72Bacterial productionBPng/l/hGiannakourou, AntoniaIncubation (INCUB)3H-Leucine incorporation in triplicated bottles incubated in the laboratory in the dark at in situ temperature over 2h
73Bacterial production, standard errorBP std e±Giannakourou, AntoniaIncubation (INCUB)3H-Leucine incorporation in triplicated bottles incubated in the laboratory in the dark at in situ temperature over 2h
74Nitrogen fixation rateN2 fixnmol/l/dayRees, AndrewIncubation (INCUB)15N-N2 addition to 2 litre samples in clear polycarbonate bottles. 24 hour incubation (dawn to dawn) in situ at 6m depth. Filtration onto 25mm GF/F, 15N and PN analysis by continuous flow stable-isotope ratio mass spectrometry
75Viral abundanceVirus#/mlPitta, ParaskeviFlow cytometryTotal
76Virus, low DNA fluorescenceVirus LDNA#/mlPitta, ParaskeviFlow cytometry
77Virus, high DNA fluorescenceVirus HDNA#/mlPitta, ParaskeviFlow cytometry
78Virus, high DNA fluorescenceVirus HDNA#/mlPitta, ParaskeviFlow cytometry
79SynechococcusSynechococcus#/mlPitta, ParaskeviFlow cytometry
80Picoeukaryotes, autotrophicPEuk auto#/mlPitta, ParaskeviFlow cytometry
81BacteriaBact#/mlPitta, ParaskeviFlow cytometry
82Bacteria, low DNA fluorescenceBact LDNA#/mlPitta, ParaskeviFlow cytometry
83Bacteria, high DNA fluorescenceBact HDNA#/mlPitta, ParaskeviFlow cytometry
84Gross primary production, 18OGPP 18Oµmol/l/dayMaugendre, LaureIncubation (INCUB)Incubated in situ at 6m depth in 5 x 60ml glass bottles bottles over sunlight duration
85Gross primary production, 18O, standard errorGPP 18O std e±Maugendre, LaureCalculated using error propagation
86Nitrate[NO3]-µmol/lLouis, JustineSpectrophotometric method with Liquid Waveguide Capillary Cell (LWCC)
87Phosphate[PO4]3-µmol/lLouis, JustineSpectrophotometric method with Liquid Waveguide Capillary Cell (LWCC)
88Nitrogen, organic, dissolvedDONµmol/lLouis, JustineUV light photo oxidation then spectrophotometric method with LWCCcalculated as the difference between total dissolved nitrogen measured (TDN) and nitrate concentration
89Phosphate, organic, dissolvedDOPµmol/lLouis, JustineUV light photo oxidation then spectrophotometric method with LWCCcalculated as the difference between total dissolved phosphorus measured (TDP) and phosphate concentration
90Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
91pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)Total scale
92Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
93Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
94Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
95Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
96Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
97Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
98Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
105103 data points

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