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Hypercapnia induced shifts in gill energy budgets of Antarctic notothenioids

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Abstract

Mechanisms responsive to hypercapnia (elevated CO2 concentrations) and shaping branchial energy turnover were investigated in isolated perfused gills of two Antarctic Notothenioids (Gobionotothen gibberifrons, Notothenia coriiceps). Branchial oxygen consumption was measured under normo- versus hypercapnic conditions (10,000 ppm CO2) at high extracellular pH values. The fractional costs of ion regulation, protein and RNA synthesis in the energy budgets were determined using specific inhibitors. Overall gill energy turnover was maintained under pH compensated hypercapnia in both Antarctic species as well as in a temperate zoarcid (Zoarces viviparus). However, fractional energy consumption by the examined processes rose drastically in G. gibberifrons (100–180%), and to a lesser extent in N. coriiceps gills (7–56%). In conclusion, high CO2 concentrations under conditions of compensated acidosis induce cost increments in epithelial processes, however, at maintained overall rates of branchial energy turnover.

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Acknowledgments

The authors would like to thank Zora Zittier, Olaf Heilmeyer, Karl-Hermann Kock and his group and the Crew of Jubany Station and RV ‘Polarstern’ during cruise ANTXXIII/8 for their unfailing and excellent help in fish catching, maintenance and preparation. Furthermore, we want to thank Erich Dunker for constructing the sophisticated respiration chambers and Gijs de Rue for expert support with the technical drawings. This work is a contribution to the “European Project on Ocean Acidification” (EPOCA) which received funding from the European Community’s Seventh Framework Programme (FP7/2007-2013) under grant agreement no. 211384. It is also a contribution to the PACES research program of the Alfred Wegener Institute and the BIOACD program funded by the Federal Ministry of Research, Germany. The study was supported by a student grant of the University of Bremen.

All animal experiments were conducted following German legislation. An approval of the work was issued by competent German authority (Freie Hansestadt Bremen, reference number 522-27-11/2-0; 28 November 2002).

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  1. Alfred Wegener Institute for Polar and Marine Research, Integrative Ecophysiology, Am Handelshafen 12, 27570, Bremerhaven, Germany

    Katrin Deigweiher, Timo Hirse, Christian Bock, Magnus Lucassen & Hans O. Pörtner

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  1. Katrin Deigweiher
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Deigweiher, K., Hirse, T., Bock, C. et al. Hypercapnia induced shifts in gill energy budgets of Antarctic notothenioids. J Comp Physiol B 180, 347–359 (2010). https://doi.org/10.1007/s00360-009-0413-x

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