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Impacts of temperature and acidification on larval calcium incorporation of the spider crab Hyas araneus from different latitudes (54° vs. 79°N)

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The combined effects of ocean warming and acidification were compared in larvae from two populations of the cold-eurythermal spider crab Hyas araneus, from one of its southernmost populations (around Helgoland, southern North Sea, 54°N, habitat temperature 3–18°C; collection: January 2008, hatch: January–February 2008) and from one of its northernmost populations (Svalbard, North Atlantic, 79°N, habitat temperature 0–6°C; collection: July 2008, hatch: February–April 2009). Larvae were exposed to temperatures of 3, 9 and 15°C combined with present-day normocapnic (380 ppm CO2) and projected future CO2 concentrations (710 and 3,000 ppm CO2). Calcium content of whole larvae was measured in freshly hatched Zoea I and after 3, 7 and 14 days during the Megalopa stage. Significant differences between Helgoland and Svalbard Megalopae were observed at all investigated temperatures and CO2 conditions. Under 380 ppm CO2, the calcium content increased with rising temperature and age of the larvae. At 3 and 9°C, Helgoland Megalopae accumulated more calcium than Svalbard Megalopae. Elevated CO2 levels, especially 3,000 ppm, caused a reduction in larval calcium contents at 3 and 9°C in both populations. This effect set in early, at 710 ppm CO2 only in Svalbard Megalopae at 9°C. Furthermore, at 3 and 9°C Megalopae from Helgoland replenished their calcium content to normocapnic levels and more rapidly than Svalbard Megalopae. However, Svalbard Megalopae displayed higher calcium contents under 3,000 ppm CO2 at 15°C. The findings of a lower capacity for calcium incorporation in crab larvae living at the cold end of their distribution range suggests that they might be more sensitive to ocean acidification than those in temperate regions.

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Acknowledgments

This work is a contribution to the “European Project on Ocean Adification” (EPOCA) which received funding from the European Community’s Seventh Framework programme (FP7/2007–2013) under grant agreement n° 211384. EPOCA is endorsed by the International Programme LOICZ. Supported by the Mar Co Pol I research programme of the Alfred-Wegener-Institute and the BIOACID programme of the Federal Ministry of Research, Germany. We would like to thank Karin Boos, Pauline Dierichsweiler, Katharina Michael, Stefanie Schnell, Karoline Schreiber and Lena Weber for excellent technical assistance.

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  1. Alfred-Wegener-Institute for Polar and Marine Research, Am Handelshafen 12, 27570, Bremerhaven, Germany

    Kathleen Walther, Franz J. Sartoris & Hans O. Pörtner

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  1. Kathleen Walther
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Correspondence to Kathleen Walther.

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Communicated by J. P. Grassle.

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Walther, K., Sartoris, F.J. & Pörtner, H.O. Impacts of temperature and acidification on larval calcium incorporation of the spider crab Hyas araneus from different latitudes (54° vs. 79°N). Mar Biol 158, 2043–2053 (2011). https://doi.org/10.1007/s00227-011-1711-x

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